ARHGDIA antibody (pSer174)

Catalog No. ABIN1531733

Product Details anti-ARHGDIA Antibody

Target: ARHGDIA (rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA))

Binding Specificity: AA 140-189, pSer174

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGDIA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity:

ARHGDIA (Phospho-Ser174) Antibody detects endogenous levels of ARHGDIA only when phosphorylated at Ser174.

PhosphorylationH:S174 M:S174 R:S174

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ARHGDIA around the phosphorylation site of Ser174.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.159161 (NCBI Gene Symbol: ARHGDIA)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-ARHGDIA antibody (ABIN1531733)

Xiao, Lin, Ke, Lin, Lin, Lin: "14-3-3τ promotes breast cancer invasion and metastasis by inhibiting RhoGDIα." in: Molecular and cellular biology, Vol. 34, Issue 14, pp. 2635-49, (2014) (PubMed).

Target Details for ARHGDIA

Target: ARHGDIA (rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA))

Alternative Name: ARHGDIA (ARHGDIA Products)

Synonyms: GDIA1 antibody, NPHS8 antibody, RHOGDI antibody, RHOGDI-1 antibody, Bles01 antibody, zgc:55554 antibody, zgc:77681 antibody, wu:fa96g11 antibody, ARHGDIA antibody, NV15502 antibody, 5330430M07Rik antibody, Gdi-1 antibody, Rho-GDI antibody, RhoDGI antibody, RhoGDI-1 antibody, arhgdia antibody, MGC84535 antibody, gdia1 antibody, rhogdi antibody, rhogdi-1 antibody, rhogdi1 antibody, Rho GDP dissociation inhibitor alpha antibody, Rho GDP dissociation inhibitor (GDI) alpha antibody, rho GDP-dissociation inhibitor 2 antibody, Rho GDP dissociation inhibitor (GDI) alpha L homeolog antibody, ARHGDIA antibody, Arhgdia antibody, arhgdia antibody, LOC100120432 antibody, arhgdia.L antibody

Background:

Synonyms: GDIR, Rho GDI 1, Rho GDP-dissociation inhibitor 1, Rho-GDI alpha

NCBI Gene Symbol: ARHGDIA

Molecular Weight: 23 kDa

Gene ID: 396

OMIM: 601925

UniProt: P52565

Pathways: Neurotrophin Signaling Pathway