PR Domain Containing 1, with ZNF Domain (PRDM1) antibody

Antigen: PR Domain Containing 1, with ZNF Domain (PRDM1)

Synonyms: BLIMP1, PRDI-BF1, MGC118922, MGC118923, MGC118924, MGC118925, GB11750, blimp-1, CG5249, dBlimp-1, dBlimp1, DmelCG5249, nrd, ubo, prdm1, Blimp1, Blimp-1, MGC91885, zgc:91885, wu:fb99c12, ZNFPR1A1, MGC52536, PRDM1, blimp1, xblimp1, prdi-bf1

Clonality: Monoclonal (3H2-E8)

Host: Mouse

Reactivity: Human, Mouse (Murine), Pig (Porcine)

Conjugate: Un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN153174

Additional Information

Alternative name: PRDM1 / BLIMP1

Gene ID: 639, 12142

Immunogen: A fragment of the mouse Blimp-1 (residues 199-409).

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 3H2-E8

Description: Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers inits carboxy terminus. It is a transcriptional repressor that is both required and sufficientto trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1is expressed in, and may be required for terminally differentiated epithelial cells, retinalneurons and other lineage cells. It has also been implicated in the repression ofbeta-interferon (IFN-beta) and c-myc gene expression. Alternate Names: anti-BLIMP 1 antibody, anti-B Lymphocyte Induced Maturation Protein 1 antibody,anti-BLIMP1 antibody, anti-Positive Regulatory Domain I Binding Factor 1 antibody,anti-PR Domain Containing 1 antibody, anti-PRDI BF1 antibody, anti-PRDM1 antibody.

Specificity: This antibody is specific for the Blimp-1. Species Reactivity: Mouse and human (see Specific Reference). Other species have not been tested. Localization: Nuclear.

Synonyms: BLIMP1, PRDI-BF1, MGC118922, MGC118923, MGC118924, MGC118925, GB11750, blimp-1, CG5249, dBlimp-1, dBlimp1, DmelCG5249, nrd, ubo, prdm1, Blimp1, Blimp-1, MGC91885, zgc:91885, wu:fb99c12, ZNFPR1A1, MGC52536, PRDM1, blimp1, xblimp1, prdi-bf1

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 1:200 with tyramide amplification,immunofluorescence 1:200 with tyramide amplification,Western Blot 1:1,000-1:5,000,Immunocytochemistry 1:50-1:100. Positive Controls: P3X (murine plasmocytoma) cell lysates

Concentration: 1.2 mg/ml

Purification: Purified

Buffer: Preservative: 0.1% sodium azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Images

Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using ABIN153174. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) antibody (Image 2)

Publications

Lin, Wong, Calame: "Repression of c-myc transcription by Blimp-1, an inducer of terminal B cell differentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5312, pp. 596-9, 1997 (PubMed).

Angelin-Duclos, Cattoretti, Lin et al.: "Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 10, pp. 5462-71, 2000 (PubMed).

Lin, Angelin-Duclos, Kuo et al.: "Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells." in: Molecular and cellular biology, Vol. 22, Issue 13, pp. 4771-80, 2002 (PubMed).

Chang, Cattoretti, Calame: "The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development." in: Mechanisms of development, Vol. 117, Issue 1-2, pp. 305-9, 2002 (PubMed).

Cattoretti, Angelin-Duclos, Shaknovich et al.: "PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage." in: The Journal of pathology, Vol. 206, Issue 1, pp. 76-86, 2005 (PubMed).

Ohinata, Payer, OCarroll et al.: "Blimp1 is a critical determinant of the germ cell lineage in mice." in: Nature, Vol. 436, Issue 7048, pp. 207-13, 2005 (PubMed).

Severa, Coccia, Fitzgerald: "Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1." in: The Journal of biological chemistry, Vol. 281, Issue 36, pp. 26188-95, 2006 (PubMed).

Gong, Malek: "Cytokine-dependent Blimp-1 expression in activated T cells inhibits IL-2 production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 1, pp. 242-52, 2006 (PubMed).

Liu, Leboeuf, Shi et al.: "Rituximab plus CHOP (R-CHOP) overcomes PRDM1-associated resistance to chemotherapy in patients with diffuse large B-cell lymphoma." in: Blood, Vol. 110, Issue 1, pp. 339-44, 2007 (PubMed).

John, Clements, Russell et al.: "Ets-1 regulates plasma cell differentiation by interfering with the activity of the transcription factor Blimp-1." in: The Journal of biological chemistry, Vol. 283, Issue 2, pp. 951-62, 2008 (PubMed).

Robertson, Charatsi, Joyner et al.: "Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice." in: Development (Cambridge, England), Vol. 134, Issue 24, pp. 4335-45, 2007 (PubMed).