PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody

Details for Product No. ABIN153175
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Antigen
Synonyms GB11750, CG5249, Dmel\\CG5249, blimp-1, dBlimp-1, dBlimp1, Blimp-1, Blimp1, nrd, prdm1, ubo, wu:fb99c12, zgc:91885, MGC52536, PRDM1, blimp1, xblimp1, prdi-bf1, BLIMP1, PRDI-BF1, ZNFPR1A1
Epitope
AA 199-409
(21), (19), (8), (7), (7), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Pig (Porcine)
(119), (64), (44), (20), (16), (13), (5), (1)
Host
Mouse
(77), (37), (9), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(112), (77), (24), (23), (19), (14), (10), (7), (5), (4), (4), (2), (1)
Pubmed 8 references available
Quantity 0.025 mL
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Catalog No. ABIN153175
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Immunogen A fragment of mouse Blimp-1 corresponding to residues 199-409.
Clone 3H2-E8
Isotype IgG1
Purification Protein G purified
Alternative Name PRDM1 / BLIMP1
Background Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers inits carboxy terminus. It is a transcriptional repressor that is both required and sufficientto trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1is expressed in, and may be required for terminally differentiated epithelial cells, retinalneurons and other lineage cells. It has also been implicated in the repression ofbeta-interferon (IFN-beta) and c-myc gene expression. Alternate Names: anti-BLIMP 1 antibody, anti-B Lymphocyte Induced Maturation Protein 1 antibody,anti-BLIMP1 antibody, anti-Positive Regulatory Domain I Binding Factor 1 antibody,anti-PR Domain Containing 1 antibody, anti-PRDI BF1 antibody, anti-PRDM1 antibody.
Gene Symbol: PRDM1
Gene ID 639
UniProt Q60636
Application Notes This Blimp-1 (3H2-E8) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry on paraffin-embedded sections and Flow Cytometry. Immunohistochemistry-Frozen was reported in scientific literature. By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. This antibody does not immunoprecipitate. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.
Recommended dilutions: ELISA 1:100-1:2000, Flow Cytometry 8 µg/mL where cells are used at 2*10^6/mL, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry 1:200, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1:200, Immunoprecipitation, Western Blot 1:1000
Protocol Protocol specific for Blimp-1 Antibody Western Blot
1. Run ~20 micrograms of protein on a 10 % SDS-PAGE.
. Block nitrocellulose membrane with 5 % blotto in TBS-0.2 % Tween-20, overnight at 4 degrees Celcius.
. Incubate membrane with diluted anti-Blimp-1 (primary antibody, cat# NB 600-235) for 1 hour at RT, in 2 % blotto in TBS-0.2 % Tween-20.
. Wash blot 4 times for 10 minutes, each, at RT with TBS-0.2 % Tween-20.
. Incubate membrane with anti-mouse IgG1 conjugated to HRP (secondary antibody), diluted in 2 % blotto in TBS-0.2 % Tween-20, for 45 minutes at RT.
. Wash 4 times for 10 minutes, each, at RT with TBS-0.2 % Tween-20.
. Incubate membrane with chemiluminescence reagent as per vendor specification. Dry blot and cover with saran wrap.
. Expose to film.9.Develop image.Immunohistochemistry / ImmunofluorescencePlease refer to the following refernence:Cattoretti, G, et al. PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage.J Pathol. 206(1):76-86 (2005)
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using ABIN153174. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.
anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody (2) Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10µm). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.
General Lin, Wong, Calame: "Repression of c-myc transcription by Blimp-1, an inducer of terminal B cell differentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5312, pp. 596-9, 1997 (PubMed).

Angelin-Duclos, Cattoretti, Lin et al.: "Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 10, pp. 5462-71, 2000 (PubMed).

Lin, Angelin-Duclos, Kuo et al.: "Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells." in: Molecular and cellular biology, Vol. 22, Issue 13, pp. 4771-80, 2002 (PubMed).

Chang, Cattoretti, Calame: "The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development." in: Mechanisms of development, Vol. 117, Issue 1-2, pp. 305-9, 2002 (PubMed).

Ohinata, Payer, OCarroll et al.: "Blimp1 is a critical determinant of the germ cell lineage in mice." in: Nature, Vol. 436, Issue 7048, pp. 207-13, 2005 (PubMed).

Severa, Coccia, Fitzgerald: "Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1." in: The Journal of biological chemistry, Vol. 281, Issue 36, pp. 26188-95, 2006 (PubMed).

Liu, Leboeuf, Shi et al.: "Rituximab plus CHOP (R-CHOP) overcomes PRDM1-associated resistance to chemotherapy in patients with diffuse large B-cell lymphoma." in: Blood, Vol. 110, Issue 1, pp. 339-44, 2007 (PubMed).

John, Clements, Russell et al.: "Ets-1 regulates plasma cell differentiation by interfering with the activity of the transcription factor Blimp-1." in: The Journal of biological chemistry, Vol. 283, Issue 2, pp. 951-62, 2008 (PubMed).

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