Vimentin antibody (pSer56)

Catalog No. ABIN1531760

Product Details anti-Vimentin Antibody

Target: Vimentin (VIM)

Binding Specificity: AA 31-80, pSer56

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity:

Vimentin (Phospho-Ser56) Antibody detects endogenous levels of Vimentin only when phosphorylated at Ser56.

PhosphorylationH:S56

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Vimentin around the phosphorylation site of Ser56.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.642813 (NCBI Gene Symbol: VIM)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin (VIM Products)

Background:

Synonyms: VIME, vimentin

NCBI Gene Symbol: VIM

Molecular Weight: 53 kDa

Gene ID: 7431

OMIM: 193060

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis