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Nucleolin antibody (NCL)

Details for Product anti-NCL Antibody No. ABIN153181, Supplier: Log in to see
Antigen
  • ATNUC-L1
  • ATNUC-L2
  • ATRANGAP1
  • B530004O11Rik
  • C23
  • D0Nds28
  • D1Nds28
  • eif3
  • eIF3-p170
  • eif3-theta
  • eif3s10
  • F27K7.6
  • F27K7_6
  • LOC100282923
  • LOC100283058
  • ncl
  • Nucl
  • nucl
  • Nucleolin
  • nucleolin like 1
  • nucleolin like 2
  • PARALLEL 1
  • PARALLEL1-LIKE 1
  • PARL1
  • PARLL1
  • RAN GTPASE-ACTIVATING PROTEIN 1
  • tif32
  • wu:fa12d03
  • zgc:152810
Reactivity
Human, Mouse (Murine), Plant, Rat (Rattus)
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40
24
5
5
3
3
3
3
3
3
2
1
Host
Rabbit
113
104
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
14
13
9
8
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunomicroscopy (IM), Simple Western (SimWes), Western Blotting (WB)
181
106
88
69
56
48
19
13
11
4
2
1
Supplier
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Immunogen A fusion protein containing amino acids 284-709 of human Nucleolin. [UniProt# P19338]
Purification Unpurified
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Gene Symbol: NCL
Molecular Weight Theoretical MW: 106 kDa
Gene ID 4691, 17975
UniProt P19338
Research Area Chromatin and Nuclear Signaling, DNA/RNA, Transcription Factors
Application Notes Western Blot 1:4000, Simple Western 1:400, Chromatin Immunoprecipitation, Immunohistochemistry 1:50-1:100, Immunocytochemistry/Immunofluorescence 1:50-1:100, Immunohistochemistry-Paraffin 1:50-1:100, Immunomicroscopy, Electron Microscopy 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Nucleolin Antibody IHC-FFPE sections I. Deparaffinization: A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunofluorescence (IF) image for anti-Nucleolin antibody (NCL) (ABIN153181) In-situ immunofluorescent staining of PD31 murine pre-B cells using ABIN153181.
Western Blotting (WB) image for anti-Nucleolin antibody (NCL) (ABIN153181) anti-Nucleolin (NCL) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Nucleolin antibody (NCL) (ABIN153181) Detection of Nucleolin in human tonsil germinal centers using ABIN153181.
Simple Western (SimWes) image for anti-Nucleolin antibody (NCL) (ABIN153181) Simple Western: Nucleolin Antibody [ABIN153181] - Simple Western lane view shows a sp...
Immunofluorescence (IF) image for anti-Nucleolin antibody (NCL) (ABIN153181) Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [ABIN153181] - IF analysis...
Product cited in: Robijns, Molenberghs, Sieprath, Corne, Verschuuren, De Vos: "In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells." in: Scientific reports, Vol. 6, pp. 30325, 2016 (PubMed). Further details: Western Blotting

Corne, Sieprath, Vandenbussche, Mohammed, Te Lindert, Gevaert, Gabriele, Wolf, De Vos: "Deregulation of focal adhesion formation and cytoskeletal tension due to loss of A-type lamins." in: Cell adhesion & migration, pp. 1-17, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Park, Park, Yu, An, Ko, Kim: "Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay." in: Genes & development, Vol. 30, Issue 18, pp. 2093-2105, 2016 (PubMed). (Sample species: Human).

Leaderer, Cashman, Kumar-Singh: "Topical application of a G-Quartet aptamer targeting nucleolin attenuates choroidal neovascularization in a model of age-related macular degeneration." in: Experimental eye research, Vol. 140, pp. 171-8, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Vermezovic, Adamowicz, Santarpia, Rustighi, Forcato, Lucano, Massimiliano, Costanzo, Bicciato, Del Sal, dAdda di Fagagna: "Notch is a direct negative regulator of the DNA-damage response." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 417-24, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Sieprath, Corne, Nooteboom, Grootaert, Rajkovic, Buysschaert, Robijns, Broers, Ramaekers, Koopman, Willems, De Vos: "Sustained accumulation of prelamin A and depletion of lamin A/C both cause oxidative stress and mitochondrial dysfunction but induce different cell fates." in: Nucleus (Austin, Tex.), Vol. 6, Issue 3, pp. 236-46, 2015 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Stępiński: "Nucleolin level in plant root meristematic cells under chilling stress and recovery." in: Micron (Oxford, England : 1993), Vol. 43, Issue 8, pp. 870-5, 2012 (PubMed). (Sample species: Plant). Further details: Immunomicroscopy

Litchfield, Riggs, Hockenberry, Oliver, Barnhart, Cai, Pierce, Ivanova, Bates, Appana, Datta, Kulesza, McBryan, Young, Klinge: "Identification and characterization of nucleolin as a COUP-TFII coactivator of retinoic acid receptor β transcription in breast cancer cells." in: PLoS ONE, Vol. 7, Issue 5, pp. e38278, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Kume, Iizumi, Shimada, Ito, Kishi, Yamaguchi, Handa: "Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 15, Issue 4, pp. 339-49, 2010 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Levitt, Clark, Jenkins, Martens, Traynor: "Differential effect of membrane cholesterol removal on mu- and delta-opioid receptors: a parallel comparison of acute and chronic signaling to adenylyl cyclase." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22108-22, 2009 (PubMed).

González, Guo, Hurley, Sun: "Identification and characterization of nucleolin as a c-myc G-quadruplex-binding protein." in: The Journal of biological chemistry, Vol. 284, Issue 35, pp. 23622-35, 2009 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Drygin, Siddiqui-Jain, OBrien, Schwaebe, Lin, Bliesath, Ho, Proffitt, Trent, Whitten, Lim, Von Hoff, Anderes, Rice: "Anticancer activity of CX-3543: a direct inhibitor of rRNA biogenesis." in: Cancer research, Vol. 69, Issue 19, pp. 7653-61, 2009 (PubMed).

Jin, Li, Davis, Zhu, Wang, Pan, Zhang: "Identification of novel proteins associated with both alpha-synuclein and DJ-1." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 5, pp. 845-59, 2007 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Fatemi, Folsom, Reutiman, Sidwell: "Viral regulation of aquaporin 4, connexin 43, microcephalin and nucleolin." in: Schizophrenia research, Vol. 98, Issue 1-3, pp. 163-77, 2007 (PubMed).

Novoselov, Kryukov, Xu, Carlson, Hatfield, Gladyshev: "Selenoprotein H is a nucleolar thioredoxin-like protein with a unique expression pattern." in: The Journal of biological chemistry, Vol. 282, Issue 16, pp. 11960-8, 2007 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Huddleson, Ahmad, Lingrel: "Up-regulation of the KLF2 transcription factor by fluid shear stress requires nucleolin." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15121-8, 2006 (PubMed). Further details: Chromatin Immunoprecipitation

Hanakahi, Dempsey, Li, Maizels: "Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3605-10, 1997 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence