Nucleolin (NCL) antibody

Antigen: Nucleolin (NCL)

Synonyms: C23, FLJ45706, FLJ59041, Nucl, D0Nds28, D1Nds28, B530004O11Rik, MGC93572, MGC68848, ncl, Nucleolin, LOC397919, NCL, MGC152810, wu:fa12d03, zgc:152810, ATNUC-L2, ATRANGAP1, NUC-L2, nucleolin like 2, PARALLEL1-LIKE 1, PARLL1, RAN GTPASE-ACTIVATING PROTEIN 1, ATNUC-L1, F27K7.6, F27K7_6, NUC-L1, nucleolin like 1, PARALLEL 1, PARL1, eif3, tif32, eif3s10, MGC121602, eIF3-p170, eif3-theta, DKFZp469M0132, nucl, LOC100282923, LOC100283058

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Plant

Conjugate: Un-conjugated

Application: Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN153181

Additional Information

Alternative name: Nucleolin

Gene ID: 4691, 17975

UniProt: P19338

Immunogen: A fusion protein containing amino acids 284-709 of human nucleolin.

Cross-Reactivity: Human, Mouse (Murine)

Format: Serum

Description: Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein inactively dividing cells, whereas the degraded forms are relatively abundant innon-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. Itplays a role in rDNA transcription, organization, and rRNA processing. It may alter DNAorganization in response to cell cycle controls. It is also a good mammalian nucleolusmarker. Alternate Names: anti-nucl antibody, anti-protein C23 antibody, anti-NCL antibody, anti-Nucleolin antibody,anti-C23 antibody.

Specificity: This antibody is specific for nucleolin protein. Species Reactivity: Human and mouse. Other species have not been tested. Localization: Nucleoli.

Synonyms: C23, FLJ45706, FLJ59041, Nucl, D0Nds28, D1Nds28, B530004O11Rik, MGC93572, MGC68848, ncl, Nucleolin, LOC397919, NCL, MGC152810, wu:fa12d03, zgc:152810, ATNUC-L2, ATRANGAP1, NUC-L2, nucleolin like 2, PARALLEL1-LIKE 1, PARLL1, RAN GTPASE-ACTIVATING PROTEIN 1, ATNUC-L1, F27K7.6, F27K7_6, NUC-L1, nucleolin like 1, PARALLEL 1, PARL1, eif3, tif32, eif3s10, MGC121602, eIF3-p170, eif3-theta, DKFZp469M0132, nucl, LOC100282923, LOC100283058

Application Details

Application Notes: Positive Controls: PD31 murine pre-B cells for immunofluorescence and crude PD31 nuclear extracts forWestern Blot.

Purification: Serum

Buffer: Preservative: 0.1% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA, Transcription Factors

Restrictions: For Research Use only

Images

In-situ immunofluorescent staining of PD31 murine pre-B cells using ABIN153181.

anti-Nucleolin (NCL) antibody (Image 2)

Detection of Nucleolin in human tonsil germinal centers using ABIN153181.

Publications

Hanakahi, Dempsey, Li et al.: "Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3605-10, 1997 (PubMed).

Huddleson, Ahmad, Lingrel: "Up-regulation of the KLF2 transcription factor by fluid shear stress requires nucleolin." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15121-8, 2006 (PubMed).

Jin, Li, Davis et al.: "Identification of novel proteins associated with both alpha-synuclein and DJ-1." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 5, pp. 845-59, 2007 (PubMed).