FOXP3 antibody (Forkhead Box P3) (C-Term)

Details for Product anti-FOXP3 Antibody No. ABIN153185, Supplier: Log in to see
Antigen
  • foxp3
  • FOXP3
  • AIID
  • DIETER
  • IPEX
  • PIDX
  • XPID
  • JM2
  • scurfin
  • sf
  • RGD1562112
  • forkhead box P3
  • forkhead box P3a
  • transcription factor foxp3
  • FOXP3
  • foxp3a
  • LOC100348270
  • foxp3
  • Foxp3
Alternatives
anti-Mouse (Murine) FOXP3 antibody for Confocal Microscopy
Epitope
C-Term, AA 400-431
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Reactivity
Human, Mouse (Murine)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This FOXP3 antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
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Immunogen A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400-431). [UniProt# Q9BZS1]
Purification Immunogen affinity purified
Alternative Name FoxP3 (FOXP3 Antibody Abstract)
Background Gene Symbol: FOXP3
Molecular Weight Theoretical MW: 47.2 kDa
Gene ID 50943
UniProt Q9BZS1
Pathways Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Application Notes Western Blot 1:1000-1:5000, Immunohistochemistry 1:50-1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:200 - 1:1000, Immunohistochemistry-Frozen 1:50This FOXP3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western Blot. By Western blot, this antibody recognizes bands at ~47.2 kDa representing FOXP3. For IHC-Fr it has been used in mouse thymus from an influenza-infected mouse or thymus from healthy mice (C57BL/6). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for FOXP3 Antibody Protocol for Foxp3 staining.- Cut 5 mm sections in a cryostat and fix them in cold acetone for 10 minutes- Dry the slides completely at room temperature (30 minutes)- Permeabilize tissues by immersing them in 0.1 % saponin, 3 times (5 minutes each time)-Block unspecific binding to the slides using 5 % normal donkey serum, 5 % normal rat serum and Fc block (1:500). Everything has to be dissolved in 0.1 % saponin-Incubate slides in a wet chamber at RT for 30 minutes-Dip off the blocking solution from slides and add streptavidin (0.1 mg/ml) for 15 minutes at RT-Wash one time with 0.1 % saponin-Add a similar volume of biotin (0.1 mg/ml) and incubate 15 min at RT- Wash again with 0.1 % saponin- Add rabbit anti-Foxp3 (1:150) and biotin-rat anti-mouse CD4 (1:100) and incubate ON at RT- Next day: Wash slides with PBS and add SA-594 or SA-488 (dissolved in PBS) to detect CD4 T cells (Dilution 1:500 from molecular probes). Incubate 1 h at RT-Wash one time with 0.1 % saponin and add biotin-donkey anti rabbit (Fab, Jackson Immunoresearch). Secondary antibody is diluted in PBS 1:500 and incubated by 3 hrs.- Wash with saponin again and add SA-488 or SA-594 (1:500 in PBS). Incubate 1 h at RT- Wash with PBS 3 times (10 minutes each wash)- Mount slides with prolong gold antifade (Molecular probes)Control: Incubated only with CD4 and no Foxp3Tissue: thymus from an Influenza-infected mouse or thymus from healthy mice (C57BL/6)
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-FOXP3 antibody (Forkhead Box P3) (C-Term) (ABIN153185) anti-Forkhead Box P3 (FOXP3) (AA 400-431), (C-Term) antibody
Immunofluorescence (IF) image for anti-FOXP3 antibody (Forkhead Box P3) (C-Term) (ABIN153185) Incubated only with CD4 and no Foxp3
Immunofluorescence (IF) image for anti-FOXP3 antibody (Forkhead Box P3) (C-Term) (ABIN153185) Sample: Healthy thymus from a C57BL/6 mouse
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-FOXP3 antibody (Forkhead Box P3) (C-Term) (ABIN153185) Immunohistochemistry-Paraffin: FoxP3 Antibody [ABIN153185] - Fox P3 was detected in i...
Product cited in: Rahman, Gudetta, Fink, Granath, Ashenafi, Aseffa, Derbew, Svensson, Andersson, Brighenti: "Compartmentalization of immune responses in human tuberculosis: few CD8+ effector T cells but elevated levels of FoxP3+ regulatory t cells in the granulomatous lesions." in: The American journal of pathology, Vol. 174, Issue 6, pp. 2211-24, 2009 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Ostroukhova, Qi, Oriss, Dixon-McCarthy, Ray, Ray: "Treg-mediated immunosuppression involves activation of the Notch-HES1 axis by membrane-bound TGF-beta." in: The Journal of clinical investigation, Vol. 116, Issue 4, pp. 996-1004, 2006 (PubMed).

Ochando, Yopp, Yang, Garin, Li, Boros, Llodra, Ding, Lira, Krieger, Bromberg: "Lymph node occupancy is required for the peripheral development of alloantigen-specific Foxp3+ regulatory T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 6993-7005, 2005 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Ochs, Ziegler, Torgerson: "FOXP3 acts as a rheostat of the immune response." in: Immunological reviews, Vol. 203, pp. 156-64, 2005 (PubMed).

Polanczyk, Carson, Subramanian, Afentoulis, Vandenbark, Ziegler, Offner: "Cutting edge: estrogen drives expansion of the CD4+CD25+ regulatory T cell compartment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 4, pp. 2227-30, 2004 (PubMed).