HSF1 antibody (pThr142)

Catalog No. ABIN1532015

Product Details anti-HSF1 Antibody

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Binding Specificity: AA 108-157, pThr142

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Specificity:

HSF1 (Phospho-Thr142) Antibody detects endogenous levels of HSF1 only when phosphorylated at Thr142.

PhosphorylationH:T142 M:T142

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Thr142.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.530227, Hs.571397 (NCBI Gene Symbol: HSF1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-HSF1 antibody (ABIN1532015)

Tamaru, Hattori, Ninomiya, Kawamura, Varès, Honda, Mishra, Wang, Benjamin, Sassone-Corsi, Ozawa, Takamatsu: "ROS Stress Resets Circadian Clocks to Coordinate Pro-Survival Signals." in: PLoS ONE, Vol. 8, Issue 12, pp. e82006, (2013) (PubMed).

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1 (HSF1 Products)

Synonyms: hsf1 antibody, Hsf antibody, xhsf1 antibody, AA960185 antibody, HSTF1 antibody, HSF1 antibody, HSF antibody, HSF1c antibody, hsf1a antibody, hsf1b antibody, zHSF1a antibody, zHSF1b antibody, hstf1 antibody, heat shock factor antibody, Heat Shock Factor antibody, heat shock factor 1 antibody, heat shock transcription factor 1 antibody, heat shock transcription factor 1 S homeolog antibody, hsf1 antibody, hsf-1 antibody, Hsf1 antibody, HSF1 antibody, hsf1.S antibody

Background:

Synonyms: Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, HSTF 1, HSTF1

NCBI Gene Symbol: HSF1

Molecular Weight: 57 kDa

Gene ID: 3297

OMIM: 140580

UniProt: Q00613