ITCH antibody (pTyr420)

Catalog No. ABIN1532110

Product Details anti-ITCH Antibody

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Binding Specificity: AA 386-435, pTyr420

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITCH antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Specificity:

ITCH (Phospho-Tyr420) Antibody detects endogenous levels of ITCH only when phosphorylated at Tyr420.

PhosphorylationH:Y420 M:Y381

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.632272 (NCBI Gene Symbol: ITCH)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH (ITCH Products)

Synonyms: AIF4 antibody, AIP4 antibody, NAPP1 antibody, dJ468O1.1 antibody, 6720481N21Rik antibody, 8030492O04Rik antibody, A130065M08 antibody, C230047C07Rik antibody, itchy E3 ubiquitin protein ligase antibody, itchy, E3 ubiquitin protein ligase antibody, ITCH antibody, Itch antibody

Background:

Synonyms: AIF4, AIP4, atrophin-1-interacting protein 4, Itchy homolog E3 ubiquitin protein ligase, NAPP1, NFE2-associated polypeptide 1, ubiquitin protein ligase ITCH

NCBI Gene Symbol: ITCH

Molecular Weight: 102 kDa

Gene ID: 83737

OMIM: 606409

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events