WNK4 antibody (WNK Lysine Deficient Protein Kinase 4) (AA 200-300)

Details for Product anti-WNK4 Antibody No. ABIN153223, Supplier: Log in to see
Antigen
  • WNK4
  • PHA2B
  • PRKWNK4
  • 2010002J11Rik
  • Pha2b
  • Prkwnk4
  • Ac2-059
  • WNK lysine deficient protein kinase 4
  • WNK4
  • Wnk4
Epitope
AA 200-300
18
12
12
7
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
72
40
38
5
1
Host
Rabbit
71
2
Clonality
Polyclonal
Conjugate
This WNK4 antibody is un-conjugated
6
6
6
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Electron Microscopy (EM), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
35
30
20
13
4
4
1
Options
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Immunogen A synthetic peptide made to a region of the human WNK4 protein between residues 200-300. [UniProt# Q96J92]
Purification Immunogen affinity purified
Alternative Name WNK4 (WNK4 Antibody Abstract)
Background Gene Symbol: WNK4
Gene ID 65266
UniProt Q96J92
Research Area Signaling
Application Notes Western Blot 2 μg/mL, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Electron MicroscopyThis WNK4 antibody is useful for Western blot where a band is seen at ~135-155 kDa. Immunohistochemistry-Frozen was reported in scientific literature. It is not useful for Immunocytochemistry/Immunofluorescence. Use in Electron Microscopy reported in scientific literature (PMID 24434184) Use in Immunohistochemistry-paraffin reported in scientific literature (PMID 24434184)

Not for use in: ICC/IF
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for WNK4 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 50 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-WNK4 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Pierce's ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-WNK4 antibody (WNK Lysine Deficient Protein Kinase 4) (AA 200-300) (ABIN153223) Detection of WNK4 in mouse kidney lysate using ABIN153223.
Western Blotting (WB) image for anti-WNK4 antibody (WNK Lysine Deficient Protein Kinase 4) (AA 200-300) (ABIN153223) Western Blot: WNK4 Antibody [ABIN153223] - Western Blot Image of anti-WNK4. Whole cel...
Product cited in: Andrukhova, Smorodchenko, Egerbacher, Streicher, Zeitz, Goetz, Shalhoub, Mohammadi, Pohl, Lanske, Erben: "FGF23 promotes renal calcium reabsorption through the TRPV5 channel." in: The EMBO journal, Vol. 33, Issue 3, pp. 229-46, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunoprecipitation,Immunoelectron Microscopy

Ohno, Uchida, Ohashi, Nitta, Ohta, Chiga, Sasaki, Uchida: "Immunolocalization of WNK4 in mouse kidney." in: Histochemistry and cell biology, Vol. 136, Issue 1, pp. 25-35, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Uchida: "Pathophysiological roles of WNK kinases in the kidney." in: Pflügers Archiv : European journal of physiology, Vol. 460, Issue 4, pp. 695-702, 2010 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Subramanya, Liu, Ellison, Wade, Welling: "WNK4 diverts the thiazide-sensitive NaCl cotransporter to the lysosome and stimulates AP-3 interaction." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18471-80, 2009 (PubMed). Further details: Western Blotting

Song, Hu, Riazi, Tiwari, Wade, Ecelbarger: "Regulation of blood pressure, the epithelial sodium channel (ENaC), and other key renal sodium transporters by chronic insulin infusion in rats." in: American journal of physiology. Renal physiology, Vol. 290, Issue 5, pp. F1055-64, 2006 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Kahle, Gimenez, Hassan, Wilson, Wong, Forbush, Aronson, Lifton: "WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 7, pp. 2064-9, 2004 (PubMed). Further details: Western Blotting,Immunohistochemistry (Frozen Sections)

Background publications Wilson, Disse-Nicodème, Choate, Ishikawa, Nelson-Williams, Desitter, Gunel, Milford, Lipkin, Achard, Feely, Dussol, Berland, Unwin, Mayan, Simon, Farfel, Jeunemaitre, Lifton: "Human hypertension caused by mutations in WNK kinases." in: Science (New York, N.Y.), Vol. 293, Issue 5532, pp. 1107-12, 2001 (PubMed).