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WNK Lysine Deficient Protein Kinase 4 (WNK4) (AA 200-300) antibody

Details for Product No. ABIN153223
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Antigen
Synonyms WNK4, PHA2B, PRKWNK4, 2010002J11Rik, Pha2b, Prkwnk4, Ac2-059
Epitope
AA 200-300
(6), (5), (4), (4), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(30), (19), (18)
Host
Rabbit
(32), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
(15), (12), (10), (5), (4)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN153223
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Immunogen A synthetic peptide made to a region of the human WNK4 protein between residues 200-300.
Purification affinity purified
Alternative Name WNK4
Background WNK kinases play an important role in hypertension. WNK4 localizes to both tightjunctions and to a segment of kidney involved in salt, potassium, and pH homeostasis,the distal nephron.
Gene Symbol: WNK4
Gene ID 65266
UniProt Q96J92
Research Area Signaling
Application Notes This WNK4 antibody is useful for Western blot where a band is seen at ~135-155 kDa. Immunohistochemistry-Frozen was reported in scientific literature. It is not useful for Immunocytochemistry/Immunofluorescence. Use in Electron Microscopy reported in scientific literature (PMID 24434184) Use in Immunohistochemistry-paraffin reported in scientific literature (PMID 24434184)
Protocol Protocol specific for WNK4 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 50 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-WNK4 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Pierce's ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.92 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-WNK Lysine Deficient Protein Kinase 4 (WNK4) (AA 200-300) antibody Detection of WNK4 in mouse kidney lysate using ABIN153223.
General Wilson, Disse-Nicodème, Choate et al.: "Human hypertension caused by mutations in WNK kinases." in: Science (New York, N.Y.), Vol. 293, Issue 5532, pp. 1107-12, 2001 (PubMed).

Kahle, Gimenez, Hassan et al.: "WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 7, pp. 2064-9, 2004 (PubMed).

Song, Hu, Riazi et al.: "Regulation of blood pressure, the epithelial sodium channel (ENaC), and other key renal sodium transporters by chronic insulin infusion in rats." in: American journal of physiology. Renal physiology, Vol. 290, Issue 5, pp. F1055-64, 2006 (PubMed).

Validation Images
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