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GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN153226, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
639
5
4
2
2
2
1
1
Host
Rabbit
320
169
87
43
16
8
8
2
Clonality
Polyclonal
Conjugate
This GFP antibody is un-conjugated
34
32
29
16
14
9
9
8
7
7
6
6
6
5
5
5
5
5
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
529
346
191
140
134
129
78
69
56
26
15
13
10
8
8
6
5
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
Options
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Immunogen Highly purified recombinant full length protein made in E. coli. The antibody is directed against the entire GFP molecule.
Specificity GFP from Aequorea victoria
Purification Immunogen affinity purified
Alternative Name GFP
Background Gene Symbol: GFP
Molecular Weight Theoretical MW: 27 kDa
Research Area Tags/Labels
Application Notes Western Blot 1:1000 - 1:3000, Flow Cytometry 1:10 - 1:1000, ELISA 1:10000 - 1:40000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Electron MicroscopyImmunoprecipitation reported successful by customer review. Immunohistochemistry-Paraffin (PMID: 21311774) Immunocytochemistry/Immunofluorescence was reported in scientific literature.Use in Flow Cytometry reported in scientific literature (PMID: 23903657). Use in Electron Microscopy reported in scientific literature (PMID 25538186). Use in Immunohistochemistry-Frozen reported in multiple pieces of scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 6.0 mg/mL
Buffer 20 mM Potassium Phosphate ( pH 7.2) and 0.15M NaCl
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Recombinant GFP detected using ABIN153226. 10 second exposure.
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Western Blot: GFP Antibody [ABIN153226] - Analysis using Biotin conjugate of ABIN1532...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Western Blot: GFP Antibody [ABIN153226] - Analysis of GFP HRP expression in HeLa/GFP ...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Western Blot: GFP Antibody [ABIN153226] - Analysis using the FITC conjugate of ABIN15...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Western Blot: GFP Antibody [ABIN153226] - Lane 1: 293FT cells transfected with CDK4 d...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Western Blot: GFP Antibody [ABIN153226] - 50ng of GFP HRP conjugate.
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Western Blot: GFP Antibody [ABIN153226] - WB analysis of GFP in various lysates. Imag...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Western Blot: GFP Antibody [ABIN153226] - Lane 1: Wild type GFP (0.1 ug ) was used to...
Immunoprecipitation (IP) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Immunoprecipitation: GFP Antibody [ABIN153226] - IP analysis of GFP in HeLa cell lysa...
Flow Cytometry (FACS) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Flow Cytometry: GFP Antibody [ABIN153226] - Analysis using the Biotin conjugate of AB...
Flow Cytometry (FACS) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153226) Flow Cytometry: GFP Antibody [ABIN153226] - Analysis of FITC conjugate of ABIN153226....
Product cited in: Brunk, Zhu, Bärenz, Kratz, Haselmann-Weiss, Antony, Hoffmann: "Cep78 is a new centriolar protein involved in Plk4-induced centriole overduplication." in: Journal of cell science, Vol. 129, Issue 14, pp. 2713-8, 2016 (PubMed).

Magarin, Pohl, Lill, Schulz, Blaschke, Heuser, Thierfelder, Donath, Drenckhahn: "Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stress." in: Journal of molecular and cellular cardiology, Vol. 97, pp. 1-14, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Jain, Beutel, Ebell, Korneev, Holthuis: "Diverting CERT-mediated ceramide transport to mitochondria triggers Bax-dependent apoptosis." in: Journal of cell science, Vol. 130, Issue 2, pp. 360-371, 2016 (PubMed).

Wang, Blumwald: "Stress-induced chloroplast degradation in Arabidopsis is regulated via a process independent of autophagy and senescence-associated vacuoles." in: The Plant cell, Vol. 26, Issue 12, pp. 4875-88, 2015 (PubMed). (Sample species: Plant). Further details: Immunoelectron Microscopy

Kratz, Bärenz, Richter, Hoffmann: "Plk4-dependent phosphorylation of STIL is required for centriole duplication." in: Biology open, Vol. 4, Issue 3, pp. 370-7, 2015 (PubMed).

Reguera, Bassil, Tajima, Wimmer, Chanoca, Otegui, Paris, Blumwald: "pH Regulation by NHX-Type Antiporters Is Required for Receptor-Mediated Protein Trafficking to the Vacuole in Arabidopsis." in: The Plant cell, Vol. 27, Issue 4, pp. 1200-17, 2015 (PubMed). Further details: Western Blotting

Hammarén, Oksanen, Nisula, Luukinen, Pesu, Rämet, Parikka: "Adequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish." in: PLoS pathogens, Vol. 10, Issue 6, pp. e1004190, 2014 (PubMed). Further details: Western Blotting

Baumann, Fernández-Sáiz, Rudelius, Lemeer, Rad, Knorn, Slawska, Engel, Jeremias, Li, Tomiatti, Illert, Targosz, Braun, Perner, Leitges, Klapper, Dreyling, Miething, Lenz, Rosenwald, Peschel, Keller et al.: "Disruption of the PRKCD-FBXO25-HAX-1 axis attenuates the apoptotic response and drives lymphomagenesis. ..." in: Nature medicine, Vol. 20, Issue 12, pp. 1401-9, 2014 (PubMed).

Macro, Jaiswal, Simon: "Dynamics of clathrin-mediated endocytosis and its requirement for organelle biogenesis in Dictyostelium." in: Journal of cell science, Vol. 125, Issue Pt 23, pp. 5721-32, 2013 (PubMed).

Zhu, Settele, Kotak, Sanchez-Pulido, Ehret, Ponting, Gönczy, Hoffmann: "MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 773-87, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Zhou, Geng, Spence, Geng: "Induction of intestinal stem cells by R-spondin 1 and Slit2 augments chemoradioprotection." in: Nature, Vol. 501, Issue 7465, pp. 107-11, 2013 (PubMed). (Sample species: Firefly). Further details: Flow Cytometry,Immunohistochemistry

Thalheimer, Korb, Bönicke, Wiegering, Mühling, Schneider, Koch, Riedel, Germer, Beilhack, Brändlein, Otto: "Noninvasive visualization of tumor growth in a human colorectal liver metastases xenograft model using bioluminescence in vivo imaging." in: The Journal of surgical research, Vol. 185, Issue 1, pp. 143-51, 2013 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Oksanen, Halfpenny, Sherwood, Harjula, Hammarén, Ahava, Pajula, Lahtinen, Parikka, Rämet: "An adult zebrafish model for preclinical tuberculosis vaccine development." in: Vaccine, Vol. 31, Issue 45, pp. 5202-9, 2013 (PubMed). (Sample species: Fish). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Patra, van Amerongen, Ghosh, Ricciardi, Sajjad, Novoyatleva, Mogha, Monk, Mühlfeld, Engel: "Organ-specific function of adhesion G protein-coupled receptor GPR126 is domain-dependent." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 42, pp. 16898-903, 2013 (PubMed). Further details: Western Blotting

Lund, Milev, Wong, Sanmuganantham, Woolaway, Chabot, Abou Elela, Mouland, Cochrane: "Differential effects of hnRNP D/AUF1 isoforms on HIV-1 gene expression." in: Nucleic acids research, Vol. 40, Issue 8, pp. 3663-75, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Bahtz, Seidler, Arnold, Haselmann-Weiss, Antony, Lehmann, Hoffmann: "GCP6 is a substrate of Plk4 and required for centriole duplication." in: Journal of cell science, Vol. 125, Issue Pt 2, pp. 486-96, 2012 (PubMed). (Sample species: Human).

Sparks, Perrien, Huppert, Peterson, Huppert: "Defects in hepatic Notch signaling result in disruption of the communicating intrahepatic bile duct network in mice." in: Disease models & mechanisms, Vol. 4, Issue 3, pp. 359-67, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Ray, Bell, Yan, Gu, Chung, Washington, Means: "Epithelial tissues have varying degrees of susceptibility to Kras(G12D)-initiated tumorigenesis in a mouse model." in: PLoS ONE, Vol. 6, Issue 2, pp. e16786, 2011 (PubMed).

Mazereeuw-Hautier, Leclerc, Simon, Serre, Jonca: "A novel mutation in CDSN causes peeling skin disease in a patient from Morocco." in: The British journal of dermatology, Vol. 165, Issue 5, pp. 1152-5, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Adler, Chen, Bystrup, Kaplan, Giovannone, Briley-Saebo, Young, Kattman, Mani, Laflamme, Zhu, Fayad, Keller: "The cardiomyocyte lineage is critical for optimization of stem cell therapy in a mouse model of myocardial infarction." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 4, pp. 1073-81, 2010 (PubMed).