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Details for Product No. ABIN153226

Green Fluorescent Protein (GFP) antibody

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Antigen
Reactivity
»Alternatives Aequorea victoria
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 6 references available
Catalog no. ABIN153226
Quantity 50 µL
Price
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Immunogen Highly purified recombinant full length protein made in E. coli. The antibody is directedagainst the entire GFP molecule.
Specificity Species Reactivity: Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP,YFP and EGFP.
Purification Serum
Alternative Name GFP
Background A highly versatile antibody that gives a stronger signal than other anti-GFP antibodiesavailable. On Western blot the antibody detects the GFP fraction from cell extractsexpressing recombinant GFP fusion proteins and has also been shown to be useful onmouse sections fixed with formalin. In immunocytochemistry, the antibody gives a verygood signal on recombinant YES-GFP chimeras expressed in COS cells. It is routinelyused in immunoprecipitation (IP) and IP-Western protocols and has been usedsuccessfully in HRP-immunohistochemistry (1:200) on whole-mount mouse embryos. Alternate Names: anti-Green Fluorescent Protein antibody, anti-EGFP antibody, anti-YFP antibody.
Research Area Tags/Labels
Application Notes Suggested working dilutions: immunoprecipitation **,Western Blot 1:2,500 - 1:10,000,Immunocytochemistry (Use in 4% normal serum) 1:200 - 1/1000,Immunohistochemistry-Paraffin 1:200 *,Immunogold Electron Microscopy 1:100. Positive Controls: Detects 5ng of recombinant GFP (using ECL or ECL Plus) in under one minute ofexposure to film.
Restrictions For Research Use only
Format Liquid
Concentration 6.2 mg/ml
Buffer 0.02M KPO4, 0.15M NaCl [pH 7.2]
Storage 4 °C
Day, Huang, McDuffie et al.: "Renal protection from ischemia mediated by A2A adenosine receptors on bone marrow-derived cells." in: The Journal of clinical investigation, Vol. 112, Issue 6, pp. 883-91, 2003 (PubMed).

Loewen, Fautsch, Teo et al.: "Long-term, targeted genetic modification of the aqueous humor outflow tract coupled with noninvasive imaging of gene expression in vivo." in: Investigative ophthalmology & visual science, Vol. 45, Issue 9, pp. 3091-8, 2004 (PubMed).

Day, Li, Rieger et al.: "A2A adenosine receptors on bone marrow-derived cells protect liver from ischemia-reperfusion injury." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 8, pp. 5040-6, 2005 (PubMed).

Jodele, Chantrain, Blavier et al.: "The contribution of bone marrow-derived cells to the tumor vasculature in neuroblastoma is matrix metalloproteinase-9 dependent." in: Cancer research, Vol. 65, Issue 8, pp. 3200-8, 2005 (PubMed).

Fabbri, Garzon, Cimmino et al.: "MicroRNA-29 family reverts aberrant methylation in lung cancer by targeting DNA methyltransferases 3A and 3B." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 40, pp. 15805-10, 2007 (PubMed).

Kim, Jung, Lee et al.: "Neuroligin-1 is required for normal expression of LTP and associative fear memory in the amygdala of adult animals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 26, pp. 9087-92, 2008 (PubMed).

Alternatives for antigen "Green Fluorescent Protein (GFP)", type "Antibodies"
Hosts (155), (152), (61), (27), (5), (2), (1)
Reactivities (370), (31), (11), (2), (1), (1)
Applications (319), (204), (111), (68), (58), (54), (40), (35), (29), (15), (13), (10), (9), (8), (4), (3), (2)
Conjugates (27), (23), (16), (10), (10), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (37), (27), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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