Green Fluorescent Protein (GFP) antibody

Details for Product No. ABIN153226
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Antigen
Reactivity
Aequorea victoria
(338), (31), (11), (8), (4), (2), (2), (1), (1), (1)
Host
Rabbit
(152), (149), (54), (26), (3), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(25), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(301), (156), (105), (67), (66), (38), (37), (31), (30), (29), (13), (11), (10), (10), (8), (4), (3), (2), (1)
Pubmed 6 references available
Quantity 0.05 mL
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Catalog No. ABIN153226
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Immunogen Highly purified recombinant full length protein made in E. coli. The antibody is directed against the entire GFP molecule.
Specificity GFP from Aequorea victoria
Cross-Reactivity (Details) Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP,EGFP.
Purification affinity purified
Alternative Name GFP
Background A highly versatile antibody that gives a stronger signal than other anti-GFP antibodiesavailable. On Western blot the antibody detects the GFP fraction from cell extractsexpressing recombinant GFP fusion proteins and has also been shown to be useful onmouse sections fixed with formalin. In immunocytochemistry, the antibody gives a verygood signal on recombinant YES-GFP chimeras expressed in COS cells. It is routinelyused in immunoprecipitation (IP) and IP-Western protocols and has been usedsuccessfully in HRP-immunohistochemistry (1:200) on whole-mount mouse embryos. Alternate Names: anti-Green Fluorescent Protein antibody, anti-EGFP antibody, anti-YFP antibody.
Research Area Tags/Labels
Application Notes Immunoprecipitation reported successful by customer review. Immunohistochemistry-Paraffin (PMID: 21311774) Immunocytochemistry/Immunofluorescence was reported in scientific literature.Use in Flow Cytometry reported in scientific literature (PMID: 23903657)
Recommended dilutions: ELISA 1:10000-1:40000, Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:10-1:2000, Immunoprecipitation 1:10-1:500, Western Blot 1:1000-1:3000
Restrictions For Research Use only
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-Green Fluorescent Protein (GFP) antibody Recombinant GFP detected using ABIN153226. 10 second exposure.
General Day, Huang, McDuffie et al.: "Renal protection from ischemia mediated by A2A adenosine receptors on bone marrow-derived cells." in: The Journal of clinical investigation, Vol. 112, Issue 6, pp. 883-91, 2003 (PubMed).

Loewen, Fautsch, Teo et al.: "Long-term, targeted genetic modification of the aqueous humor outflow tract coupled with noninvasive imaging of gene expression in vivo." in: Investigative ophthalmology & visual science, Vol. 45, Issue 9, pp. 3091-8, 2004 (PubMed).

Day, Li, Rieger et al.: "A2A adenosine receptors on bone marrow-derived cells protect liver from ischemia-reperfusion injury." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 8, pp. 5040-6, 2005 (PubMed).

Jodele, Chantrain, Blavier et al.: "The contribution of bone marrow-derived cells to the tumor vasculature in neuroblastoma is matrix metalloproteinase-9 dependent." in: Cancer research, Vol. 65, Issue 8, pp. 3200-8, 2005 (PubMed).

Fabbri, Garzon, Cimmino et al.: "MicroRNA-29 family reverts aberrant methylation in lung cancer by targeting DNA methyltransferases 3A and 3B." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 40, pp. 15805-10, 2007 (PubMed).

Kim, Jung, Lee et al.: "Neuroligin-1 is required for normal expression of LTP and associative fear memory in the amygdala of adult animals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 26, pp. 9087-92, 2008 (PubMed).

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