beta-Galactosidase Tag antibody

Antigen: beta-Galactosidase Tag

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), ELISA

Catalog no.: ABIN153228

Additional Information

Immunogen: Recombinant full length protein (E. coli).

Description: Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is ametalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal,non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cationsseems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activateenzyme activity based upon the substrate used. Alternate Names: anti-EC 3.2.1.23 antibody, anti-b-gal antibody.

Specificity: Species Reactivity: Cross-reacts with E. coli. Not yet tested in other species. Localization: Cytoplasmic.

Application Details

Application Notes: Suggested working dilutions: ELISA 1:4000-1:16000,immunohistochemistry 1:500 - 1:3,000,immunoprecipitation 1/500 - 1/5000,Western Blot 1/2000-1/25000. Positive Controls: 1.0g of E.coli in a standard sandwich ELISA.

Concentration: 10.0 mg/ml

Purification: Purified

Buffer: 0.02M Potassium Phosphate, 0.15M NaCl (pH 7.2) with 1 percent BSA, 50 percentglycerol. Preservative: Sodium Azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Images

anti-beta-Galactosidase Tag antibody

Publications

Struenkelnberg, Bonengel, Moda et al.: "rst and its paralogue kirre act redundantly during embryonic muscle development in Drosophila." in: Development (Cambridge, England), Vol. 128, Issue 21, pp. 4229-39, 2001 (PubMed).

Tulina, Matunis: "Control of stem cell self-renewal in Drosophila spermatogenesis by JAK-STAT signaling." in: Science (New York, N.Y.), Vol. 294, Issue 5551, pp. 2546-9, 2001 (PubMed).

Zechner, Fujita, Huelsken et al.: "beta-Catenin signals regulate cell growth and the balance between progenitor cell expansion and differentiation in the nervous system." in: Developmental biology, Vol. 258, Issue 2, pp. 406-18, 2003 (PubMed).

Condon, Jeyasuria, Faust et al.: "Surfactant protein secreted by the maturing mouse fetal lung acts as a hormone that signals the initiation of parturition." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 14, pp. 4978-83, 2004 (PubMed).

Alvarez, Serra: "Unique and redundant roles of Smad3 in TGF-beta-mediated regulation of long bone development in organ culture." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 230, Issue 4, pp. 685-99, 2004 (PubMed).

Farmen, Karp, Ng et al.: "Gene transfer of CFTR to airway epithelia: low levels of expression are sufficient to correct Cl- transport and overexpression can generate basolateral CFTR." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 289, Issue 6, pp. L1123-30, 2005 (PubMed).

Li, Sharpe, Maupin et al.: "VEGF and PlGF promote adult vasculogenesis by enhancing EPC recruitment and vessel formation at the site of tumor neovascularization." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 9, pp. 1495-7, 2006 (PubMed).