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|+1 888 205 9894 (TF)|
beta-Galactosidase Tag antibody
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), ELISA
|7 references available|
|Quantity||1.0 mg (10.0 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Immunogen||Recombinant full length protein (E. coli).|
|Description||Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is ametalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal,non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cationsseems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activateenzyme activity based upon the substrate used. Alternate Names: anti-EC 18.104.22.168 antibody, anti-b-gal antibody.|
|Specificity||Species Reactivity: Cross-reacts with E. coli. Not yet tested in other species. Localization: Cytoplasmic.|
|Application Notes||Suggested working dilutions: ELISA 1:4000-1:16000,immunohistochemistry 1:500 - 1:3,000,immunoprecipitation 1/500 - 1/5000,Western Blot 1/2000-1/25000. Positive Controls: 1.0g of E.coli in a standard sandwich ELISA.|
|Buffer||0.02M Potassium Phosphate, 0.15M NaCl (pH 7.2) with 1 percent BSA, 50 percentglycerol. Preservative: Sodium Azide.|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Restrictions||For Research Use only|
|anti-beta-Galactosidase Tag antibody|
Struenkelnberg, Bonengel, Moda et al.: "rst and its paralogue kirre act redundantly during embryonic muscle development in Drosophila." in: Development (Cambridge, England), Vol. 128, Issue 21, pp. 4229-39, 2001 (PubMed).
Tulina, Matunis: "Control of stem cell self-renewal in Drosophila spermatogenesis by JAK-STAT signaling." in: Science (New York, N.Y.), Vol. 294, Issue 5551, pp. 2546-9, 2001 (PubMed).
Zechner, Fujita, Huelsken et al.: "beta-Catenin signals regulate cell growth and the balance between progenitor cell expansion and differentiation in the nervous system." in: Developmental biology, Vol. 258, Issue 2, pp. 406-18, 2003 (PubMed).
Condon, Jeyasuria, Faust et al.: "Surfactant protein secreted by the maturing mouse fetal lung acts as a hormone that signals the initiation of parturition." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 14, pp. 4978-83, 2004 (PubMed).
Alvarez, Serra: "Unique and redundant roles of Smad3 in TGF-beta-mediated regulation of long bone development in organ culture." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 230, Issue 4, pp. 685-99, 2004 (PubMed).
Farmen, Karp, Ng et al.: "Gene transfer of CFTR to airway epithelia: low levels of expression are sufficient to correct Cl- transport and overexpression can generate basolateral CFTR." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 289, Issue 6, pp. L1123-30, 2005 (PubMed).
Li, Sharpe, Maupin et al.: "VEGF and PlGF promote adult vasculogenesis by enhancing EPC recruitment and vessel formation at the site of tumor neovascularization." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 9, pp. 1495-7, 2006 (PubMed).
|Hosts||Rabbit (9), Chicken (3), Mouse (2)|
|Reactivities||Escherichia coli (E. coli) (13)|
|Applications||Western Blotting (WB) (13), ELISA (8), Immunocytochemistry (ICC) (7), Immunofluorescence (IF) (7), Immunoprecipitation (IP) (5), Dot Blot (DB) (3), Enzyme Immunoassay (EIA) (3), Immunodiffusion (ID) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Radioimmunoassay (RIA) (3), Flow Cytometry (FACS) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)|
|Conjugates||Biotin (2), FITC (1), HRP (1)|