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beta-Galactosidase Tag antibody

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Pubmed 7 references available
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Quantity 1 mg
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Immunogen Recombinant full length protein (E. coli).
Specificity Beta-Galactosidase
Cross-Reactivity (Details) Not yet tested in other species.
Purification IgG purified
Background Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is ametalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal,non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cationsseems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activateenzyme activity based upon the substrate used. Alternate Names: anti-EC antibody, anti-b-gal antibody.
Application Notes Suitable for immunoblotting , ELISA, Immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:1,500 dilution has been reported - detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut - produce free-floating sections. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23838935)
Recommended dilutions: ELISA 1:10000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry 1:1500, Immunohistochemistry-Paraffin 1:10-1:2000, Western Blot 1:5000-1:10000
Restrictions For Research Use only
Concentration 10.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.02 M NaCl (pH 7.2), Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-beta-Galactosidase Tag antibody (ABIN153228) anti-beta-Galactosidase Tag antibody
General Li, Sharpe, Maupin et al.: "VEGF and PlGF promote adult vasculogenesis by enhancing EPC recruitment and vessel formation at the site of tumor neovascularization." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 9, pp. 1495-7, 2006 (PubMed).

Farmen, Karp, Ng et al.: "Gene transfer of CFTR to airway epithelia: low levels of expression are sufficient to correct Cl- transport and overexpression can generate basolateral CFTR." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 289, Issue 6, pp. L1123-30, 2005 (PubMed).

Alvarez, Serra: "Unique and redundant roles of Smad3 in TGF-beta-mediated regulation of long bone development in organ culture." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 230, Issue 4, pp. 685-99, 2004 (PubMed).

Condon, Jeyasuria, Faust et al.: "Surfactant protein secreted by the maturing mouse fetal lung acts as a hormone that signals the initiation of parturition." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 14, pp. 4978-83, 2004 (PubMed).

Zechner, Fujita, Huelsken et al.: "beta-Catenin signals regulate cell growth and the balance between progenitor cell expansion and differentiation in the nervous system." in: Developmental biology, Vol. 258, Issue 2, pp. 406-18, 2003 (PubMed).

Tulina, Matunis: "Control of stem cell self-renewal in Drosophila spermatogenesis by JAK-STAT signaling." in: Science (New York, N.Y.), Vol. 294, Issue 5551, pp. 2546-9, 2001 (PubMed).

Struenkelnberg, Bonengel, Moda et al.: "rst and its paralogue kirre act redundantly during embryonic muscle development in Drosophila." in: Development (Cambridge, England), Vol. 128, Issue 21, pp. 4229-39, 2001 (PubMed).

Catalog No. ABIN153228

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