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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|15 references available|
|Quantity||0.1 mg (1.1 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Immunogen||Green fluorescent Protein (GFP) fusion protein corresponding to the full length aminoacid sequence (246aa) derived from the jellyfish Aequorea victoria.|
|Description||This antibody has been developed specifically for use in applications requiring a hightiter and specificity with minimum background. Polyclonal anti-GFP is designed to detectGFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich orcapture) for the direct binding of antigen and recognizes wild type, recombinant andenhanced forms of GFP. Polyclonal anti-GFP is designed to detect GFP and itsvariants. This antibody can be used to detect GFP by ELISA (sandwich or capture) forthe direct binding of antigen and recognizes wild type, recombinant and enhanced formsof GFP. Anti-GFP can be used to detect GFP by immunofluorescence microscopy inprokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFPcontaining inserts. Significant amplification of signal is achieved using fluorochromeconjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. Forimmunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonalanti-GFP to detect GFP or GFP containing proteins on western blots. Alternate Names: anti-Green Fluorescent Protein antibody, anti-S65T-GFP antibody, anti-RS-GFPantibody, anti-YFP antibody, anti-EGFP antibody.|
|Specificity||Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP,YFP and EGFP. Species Reactivity: This antibody detects Green Fluorescent Protein.|
|Application Notes||Suggested working dilutions: immunohistochemistry 1:200-1:3000,immunofluorescence 1:200-1:2000,Western Blot 1:500- 1:5000,Immunocytochemistry|
|Purification||Aff - Purified|
|Buffer||0.02 M K Phosphate, 0.15 Sodium Chloride, pH 7.2. Preservative: 0.01% Sodium Azide.|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Restrictions||For Research Use only|
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