GFP antibody

Antigen: GFP

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN153230

Additional Information

Immunogen: Green fluorescent Protein (GFP) fusion protein corresponding to the full length aminoacid sequence (246aa) derived from the jellyfish Aequorea victoria.

Description: This antibody has been developed specifically for use in applications requiring a hightiter and specificity with minimum background. Polyclonal anti-GFP is designed to detectGFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich orcapture) for the direct binding of antigen and recognizes wild type, recombinant andenhanced forms of GFP. Polyclonal anti-GFP is designed to detect GFP and itsvariants. This antibody can be used to detect GFP by ELISA (sandwich or capture) forthe direct binding of antigen and recognizes wild type, recombinant and enhanced formsof GFP. Anti-GFP can be used to detect GFP by immunofluorescence microscopy inprokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFPcontaining inserts. Significant amplification of signal is achieved using fluorochromeconjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. Forimmunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonalanti-GFP to detect GFP or GFP containing proteins on western blots. Alternate Names: anti-Green Fluorescent Protein antibody, anti-S65T-GFP antibody, anti-RS-GFPantibody, anti-YFP antibody, anti-EGFP antibody.

Specificity: Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP,YFP and EGFP. Species Reactivity: This antibody detects Green Fluorescent Protein.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 1:200-1:3000,immunofluorescence 1:200-1:2000,Western Blot 1:500- 1:5000,Immunocytochemistry

Concentration: 1.1 mg/ml

Purification: Aff - Purified

Buffer: 0.02 M K Phosphate, 0.15 Sodium Chloride, pH 7.2. Preservative: 0.01% Sodium Azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: Tags/Labels

Restrictions: For Research Use only

Publications

Mesaeli, Nakamura, Zvaritch et al.: "Calreticulin is essential for cardiac development." in: The Journal of cell biology, Vol. 144, Issue 5, pp. 857-68, 1999 (PubMed).

Jasinska, Zhang, Pilquil et al.: "Lipid phosphate phosphohydrolase-1 degrades exogenous glycerolipid and sphingolipid phosphate esters." in: The Biochemical journal, Vol. 340 ( Pt 3), pp. 677-86, 1999 (PubMed).

McCabe, Berthiaume: "Functional roles for fatty acylated amino-terminal domains in subcellular localization." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3771-86, 1999 (PubMed).

Zhang, Pilquil, Dewald et al.: "Identification of structurally important domains of lipid phosphate phosphatase-1: implications for its sites of action." in: The Biochemical journal, Vol. 345 Pt 2, pp. 181-4, 2000 (PubMed).

Wong, Kusdra, Collins: "Subnuclear shuttling of human telomerase induced by transformation and DNA damage." in: Nature cell biology, Vol. 4, Issue 9, pp. 731-6, 2002 (PubMed).

Kootstra, Munk, Tonnu et al.: "Abrogation of postentry restriction of HIV-1-based lentiviral vector transduction in simian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 3, pp. 1298-303, 2003 (PubMed).

Kanazawa, Verma: "Little evidence of bone marrow-derived hepatocytes in the replacement of injured liver." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100 Suppl 1, pp. 11850-3, 2003 (PubMed).

Schoch, Lori, Burns et al.: "A subset of mouse tracheal epithelial basal cells generates large colonies in vitro." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 286, Issue 4, pp. L631-42, 2004 (PubMed).

Fischer, Beck, Smith et al.: "Successful transgene expression with serial doses of aerosolized rAAV2 vectors in rhesus macaques." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 8, Issue 6, pp. 918-26, 2003 (PubMed).

Gongidi, Ring, Moody et al.: "SPARC-like 1 regulates the terminal phase of radial glia-guided migration in the cerebral cortex." in: Neuron, Vol. 41, Issue 1, pp. 57-69, 2004 (PubMed).

Merkle, Tramontin, Garcuia-Verdugo et al.: "Radial glia give rise to adult neural stem cells in the subventricular zone." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 50, pp. 17528-32, 2004 (PubMed).

Day, Li, Rieger et al.: "A2A adenosine receptors on bone marrow-derived cells protect liver from ischemia-reperfusion injury." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 8, pp. 5040-6, 2005 (PubMed).

Reinhardt, Hong, Kang et al.: "Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 3, pp. 1618-27, 2006 (PubMed).

Shafikhani, Engel: "Pseudomonas aeruginosa type III-secreted toxin ExoT inhibits host-cell division by targeting cytokinesis at multiple steps." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 42, pp. 15605-10, 2006 (PubMed).

Dindot, Antalffy, Bhattacharjee et al.: "The Angelman syndrome ubiquitin ligase localizes to the synapse and nucleus, and maternal deficiency results in abnormal dendritic spine morphology." in: Human molecular genetics, Vol. 17, Issue 1, pp. 111-8, 2007 (PubMed).