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GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN153230, Supplier: Log in to see
Antigen
Reactivity
Aequorea victoria
614
5
4
3
2
2
2
1
Host
Rabbit
308
162
81
42
16
11
8
2
Clonality
Polyclonal
Conjugate
This GFP antibody is un-conjugated
31
31
26
14
12
8
8
7
6
6
5
5
5
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Free Floating) (IHC (ff)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm)), Immunomicroscopy (IM), Immunoprecipitation (IP), In situ hybridization (ISH), Western Blotting (WB)
502
319
174
139
123
112
61
58
32
26
13
12
10
9
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Immunogen Green fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Isotype IgG
Specificity Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
Purification Immunogen affinity purified
Alternative Name GFP
Background Gene Symbol: GFP
Molecular Weight Theoretical MW: 27 kDa
UniProt P42212
Research Area Tags/Labels
Application Notes Western Blot 1:500-1:5000, Chromatin Immunoprecipitation 1:10-1:500, Flow Cytometry 1:1:10 - 1:1000, ELISA 1:20000-1:120000, Immunohistochemistry 1:200-1:3000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:50-1:250, In-situ Hybridization, Immunomicroscopy, Electron Microscopy, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.99 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153230) Western Blot: GFP Antibody [ABIN153230] - analysis of GFP tagged PKA subunits over-ex...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153230) Western Blot: GFP Antibody [ABIN153230] - Lane 1: Wild type GFP (0.1 ug) was used to ...
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153230) Immunocytochemistry/Immunofluorescence: GFP Antibody [ABIN153230] - Analysis of GFP i...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153230) Western Blot: GFP Antibody [ABIN153230] - Lane 1: 293FT cells transfected with CDK4 d...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153230) Immunohistochemistry-Paraffin: GFP Antibody [ABIN153230] - Staining of GPF+ hepatocyt...
Immunoprecipitation (IP) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153230) Immunoprecipitation: GFP Antibody [ABIN153230] - IP analysis of GFP in S2 Cells (Dros...
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153230) Immunocytochemistry/Immunofluorescence: GFP Antibody [ABIN153230] - Analysis of GFP i...
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153230) Immunocytochemistry/Immunofluorescence: GFP Antibody [ABIN153230] - Analysis of GFP i...
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153230) Immunocytochemistry/Immunofluorescence: GFP Antibody [ABIN153230] - Analysis of GFP i...
Immunomicroscopy (IM) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153230) Immunomicroscopy: GFP Antibody [ABIN153230] - Monocyte derived dendritic cells and de...
Product cited in: Huang, Yang, Chen, Liu, Chang, Chuang: "Treatment of Glucocorticoids Inhibited Early Immune Responses and Impaired Cardiac Repair in Adult Zebrafish." in: PLoS ONE, Vol. 8, Issue 6, pp. e66613, 2016 (PubMed). (Sample species: Zebrafish (Danio rerio)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Rueckemann, DiMauro, Rangel, Han, Boyden, Eichenbaum: "Transient optogenetic inactivation of the medial entorhinal cortex biases the active population of hippocampal neurons." in: Hippocampus, Vol. 26, Issue 2, pp. 246-60, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)

Zeng, Guo, Xu, Wang, Feng, Long, Liang, Huang, Lu, Li, Zhou, Burgunder, Pang, Pei: "Xyloketal-derived small molecules show protective effect by decreasing mutant Huntingtin protein aggregates in Caenorhabditis elegans model of Huntington's disease." in: Drug design, development and therapy, Vol. 10, pp. 1443-51, 2016 (PubMed). (Sample species: Caenorhabditis elegans (C. elegans)). Further details: Western Blotting

Barrionuevo, Hurtado, Kim, Real, Bakkali, Kopp, Sander, Scherer, Burgos, Jiménez: "Sox9 and Sox8 protect the adult testis from male-to-female genetic reprogramming and complete degeneration." in: eLife, Vol. 5, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Liu, Lee, Sun, Lower, Runge, Ma, Wu: "Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis." in: Molecular biology of the cell, Vol. 27, Issue 16, pp. 2528-41, 2016 (PubMed). (Sample species: Jellyfish). Further details: Immunoprecipitation

Ahn, Rechsteiner, Strome, Kelly: "A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans." in: PLoS genetics, Vol. 12, Issue 8, pp. e1006227, 2016 (PubMed). Further details: Immunocytochemistry,Immunoprecipitation,Immunofluorescence

Sorrentino, Maffia, Strollo, Cacace, Romagnoli, Manfredi, Ventrella, Dondi, Barone, Giunti, Graham, Huang, Kalled, Auricchio, Bacci, Surace, Fraldi: "A Comprehensive Map of CNS Transduction by Eight Recombinant Adeno-associated Virus Serotypes Upon Cerebrospinal Fluid Administration in Pigs." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 24, Issue 2, pp. 276-86, 2016 (PubMed). Further details: Western Blotting,Immunohistochemistry (Free Floating),Immunohistochemistry (Frozen Sections)

Myeku, Clelland, Emrani, Kukushkin, Yu, Goldberg, Duff: "Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling." in: Nature medicine, Vol. 22, Issue 1, pp. 46-53, 2016 (PubMed). Further details: Western Blotting

Shiba, Gomibuchi, Seto, Wada, Ichimura, Tanaka, Ogasawara, Okada, Shiba, Sakamoto, Ido, Shiina, Ohkura, Nakai, Uno, Kazuki, Oshimura, Minami, Ikeda: "Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts." in: Nature, Vol. 538, Issue 7625, pp. 388-391, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Bao, Carr, Wu, Barclay, Jin, Ciofani, Reinhardt: "BATF Modulates the Th2 Locus Control Region and Regulates CD4+ T Cell Fate during Antihelminth Immunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 197, Issue 11, pp. 4371-4381, 2016 (PubMed).

Yovchev, Oertel: "Fetal Liver Stem/Progenitor Cell Transplantation: A Model to Study Tissue Mass Replacement and Cell-Based Therapies." in: Methods in molecular biology (Clifton, N.J.), Vol. 1506, pp. 101-115, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Lee, Rothenberg, Seeley, Zimdahl, Kawano, Lu, Shin, Sakata-Kato, Chen, Diehn, Clarke, Beachy: "Control of inflammation by stromal Hedgehog pathway activation restrains colitis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 47, pp. E7545-E7553, 2016 (PubMed). (Sample species: Human).

Meng, Ward, Chun, Bennett, Beaudet, Rigo: "Towards a therapy for Angelman syndrome by targeting a long non-coding RNA." in: Nature, Vol. 518, Issue 7539, pp. 409-12, 2015 (PubMed). Further details: Western Blotting

Davidson, Laporte, Wu: "Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis." in: Molecular biology of the cell, Vol. 26, Issue 3, pp. 453-66, 2015 (PubMed). Further details: Immunoprecipitation

Matthews, Deschênes, Furuta, Moore, Wang, Karten, Kleinfeld: "Feedback in the brainstem: an excitatory disynaptic pathway for control of whisking." in: The Journal of comparative neurology, Vol. 523, Issue 6, pp. 921-42, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Doi, Ogata, Eto, Kato, Kuno, Kinoshita, Kanayama, Feng, Manabe, Yoshimura: "Differential contributions of graft-derived and host-derived cells in tissue regeneration/remodeling after fat grafting." in: Plastic and reconstructive surgery, Vol. 135, Issue 6, pp. 1607-17, 2015 (PubMed). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Gautam, Fujita, Kimura, Ichikawa, Izawa, Hirose, Kashihara, Yamada, Takahashi, Ikeda, Shiba: "Transplantation of adipose tissue-derived stem cells improves cardiac contractile function and electrical stability in a rat myocardial infarction model." in: Journal of molecular and cellular cardiology, Vol. 81, pp. 139-49, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Gabrusiewicz, Hossain, Cortes-Santiago, Fan, Kaminska, Marini, Fueyo, Gomez-Manzano: "Macrophage Ablation Reduces M2-Like Populations and Jeopardizes Tumor Growth in a MAFIA-Based Glioma Model." in: Neoplasia (New York, N.Y.), Vol. 17, Issue 4, pp. 374-84, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Akhtar Ali, Younis, Wallerman, Gupta, Andersson, Sjöblom: "Transcriptional modulator ZBED6 affects cell cycle and growth of human colorectal cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 25, pp. 7743-8, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Gross, Balderes, Liu, Asfaha, Gu, Wang, Sussel: "Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential." in: American journal of physiology. Gastrointestinal and liver physiology, pp. ajpgi.00244.2015, 2015 (PubMed).