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GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN153230, Supplier: Login to see New
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Aequorea victoria
(496), (5), (4), (3), (2), (2), (2), (1), (1)
(217), (157), (79), (38), (11), (7), (2)
This GFP antibody is un-conjugated
(29), (28), (20), (15), (13), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), In situ hybridization (ISH), Western Blotting (WB)
(412), (235), (138), (121), (106), (41), (40), (35), (27), (26), (15), (14), (10), (9), (8), (4), (3), (2), (1), (1), (1), (1), (1)
Pubmed 15 references available
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Quantity 0.1 mg
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Immunogen Green fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Isotype IgG
Specificity Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
No Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification affinity purified
Alternative Name GFP
Background This antibody has been developed specifically for use in applications requiring a hightiter and specificity with minimum background. Polyclonal anti-GFP is designed to detectGFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich orcapture) for the direct binding of antigen and recognizes wild type, recombinant andenhanced forms of GFP. Polyclonal anti-GFP is designed to detect GFP and itsvariants. This antibody can be used to detect GFP by ELISA (sandwich or capture) forthe direct binding of antigen and recognizes wild type, recombinant and enhanced formsof GFP. Anti-GFP can be used to detect GFP by immunofluorescence microscopy inprokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFPcontaining inserts. Significant amplification of signal is achieved using fluorochromeconjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. Forimmunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonalanti-GFP to detect GFP or GFP containing proteins on western blots. Alternate Names: anti-Green Fluorescent Protein antibody, anti-S65T-GFP antibody, anti-RS-GFPantibody, anti-YFP antibody, anti-EGFP antibody.
Research Area Tags/Labels
Application Notes Immunofluorescence (PMID: 21855203) Immunoprecipitation (PMID: 20427314 and PMID: 22298427) Chromatin Immunoprecipitation (PMID: 21952049) In-situ Hybridization (PMID: 22340499) Immunohistochemistry- frozen (PMID: 22464327) Use in Electron Microscopy reported in scientific literature (PMID: 23873149)
Recommended dilutions: Chromatin Immunoprecipitation 1:10-1:500, Electron Microscopy, ELISA 1:20000-1:120000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:200-1:3000, Immunohistochemistry-Frozen 1:50-1:250, Immunohistochemistry-Paraffin 1:10-1:500, Immunoprecipitation 1:10-1:500, In-situ Hybridization, Western Blot 1:500-1:5000
Restrictions For Research Use only
Concentration 1.02 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Product cited in: Dindot, Antalffy, Bhattacharjee et al.: "The Angelman syndrome ubiquitin ligase localizes to the synapse and nucleus, and maternal deficiency results in abnormal dendritic spine morphology." in: Human molecular genetics, Vol. 17, Issue 1, pp. 111-8, 2007 (PubMed).

Shafikhani, Engel: "Pseudomonas aeruginosa type III-secreted toxin ExoT inhibits host-cell division by targeting cytokinesis at multiple steps." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 42, pp. 15605-10, 2006 (PubMed).

Reinhardt, Hong, Kang et al.: "Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 3, pp. 1618-27, 2006 (PubMed).

Day, Li, Rieger et al.: "A2A adenosine receptors on bone marrow-derived cells protect liver from ischemia-reperfusion injury." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 8, pp. 5040-6, 2005 (PubMed).

Merkle, Tramontin, Garcuia-Verdugo et al.: "Radial glia give rise to adult neural stem cells in the subventricular zone." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 50, pp. 17528-32, 2004 (PubMed).

Gongidi, Ring, Moody et al.: "SPARC-like 1 regulates the terminal phase of radial glia-guided migration in the cerebral cortex." in: Neuron, Vol. 41, Issue 1, pp. 57-69, 2004 (PubMed).

Fischer, Beck, Smith et al.: "Successful transgene expression with serial doses of aerosolized rAAV2 vectors in rhesus macaques." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 8, Issue 6, pp. 918-26, 2003 (PubMed).

Schoch, Lori, Burns et al.: "A subset of mouse tracheal epithelial basal cells generates large colonies in vitro." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 286, Issue 4, pp. L631-42, 2004 (PubMed).

Kootstra, Munk, Tonnu et al.: "Abrogation of postentry restriction of HIV-1-based lentiviral vector transduction in simian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 3, pp. 1298-303, 2003 (PubMed).

Wong, Kusdra, Collins: "Subnuclear shuttling of human telomerase induced by transformation and DNA damage." in: Nature cell biology, Vol. 4, Issue 9, pp. 731-6, 2002 (PubMed).

Background publications Kanazawa, Verma: "Little evidence of bone marrow-derived hepatocytes in the replacement of injured liver." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100 Suppl 1, pp. 11850-3, 2003 (PubMed).

Zhang, Pilquil, Dewald et al.: "Identification of structurally important domains of lipid phosphate phosphatase-1: implications for its sites of action." in: The Biochemical journal, Vol. 345 Pt 2, pp. 181-4, 2000 (PubMed).

McCabe, Berthiaume: "Functional roles for fatty acylated amino-terminal domains in subcellular localization." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3771-86, 1999 (PubMed).

Jasinska, Zhang, Pilquil et al.: "Lipid phosphate phosphohydrolase-1 degrades exogenous glycerolipid and sphingolipid phosphate esters." in: The Biochemical journal, Vol. 340 ( Pt 3), pp. 677-86, 1999 (PubMed).

Mesaeli, Nakamura, Zvaritch et al.: "Calreticulin is essential for cardiac development." in: The Journal of cell biology, Vol. 144, Issue 5, pp. 857-68, 1999 (PubMed).

Catalog No. ABIN153230
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