V5 Epitope Tag antibody

Details for Product No. ABIN153299, Supplier: Log in to see
Antigen
Reactivity
Tag
403
18
16
8
3
3
Host
Goat
249
144
36
7
5
4
3
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
31
28
21
14
13
12
12
12
11
10
10
10
10
9
8
8
7
6
5
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
369
263
136
94
77
71
53
44
28
13
9
8
5
4
4
2
2
2
2
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Goats were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype IgG
Purification Immunogen affinity purified
Alternative Name Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Target Type Tag
Research Area Tags/Labels
Application Notes Western Blot 1:1000 - 1:30000, ELISA Primary-1:1000-1:30000, Coating plates-1:100-1:500, Immunocytochemistry/Immunofluorescence 1:100-1:400, Immunoprecipitation 1 - 4 μg/mg lysateUse in immunprecipitation reported in scientific literature (PMID 25767115)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (ABIN153299) Western Blot: V5 Epitope Tag Antibody [ABIN153299] - Analysis using the Biotin conjug...
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (ABIN153299) Western Blot: V5 Epitope Tag Antibody [ABIN153299] - Analysis using the HRP conjugate...
Product cited in: Heidrich, Carattino, Hughey, Pilewski, Kleyman, Myerburg: "Intracellular Na+ regulates epithelial Na+ channel maturation." in: The Journal of biological chemistry, Vol. 290, Issue 18, pp. 11569-77, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunoprecipitation

Butterworth, Zhang, Liu, Shanks, Thibodeau: "Modulation of the epithelial sodium channel (ENaC) by bacterial metalloproteases and protease inhibitors." in: PLoS ONE, Vol. 9, Issue 6, pp. e100313, 2014 (PubMed). Further details: Immunoprecipitation

Liu, Yu, Jin, Han, Lendahl, Zhao, Nistér: "The mitochondrial elongation factors MIEF1 and MIEF2 exert partially distinct functions in mitochondrial dynamics." in: Experimental cell research, Vol. 319, Issue 18, pp. 2893-904, 2013 (PubMed). Method employed by authors: Immunoprecipitation (IP)

Courtney, Di, Stockman, Liu, Scherbik, Brinton: "Identification of novel host cell binding partners of Oas1b, the protein conferring resistance to flavivirus-induced disease in mice." in: Journal of virology, Vol. 86, Issue 15, pp. 7953-63, 2012 (PubMed).

Pempe, Xu, Gopalakrishnan, Liu, Harris: "Probing structural selectivity of synthetic heparin binding to Stabilin protein receptors." in: The Journal of biological chemistry, Vol. 287, Issue 25, pp. 20774-83, 2012 (PubMed).

Ikeda, Andoo, Shimono, Takamatsu, Taki, Muta, Matsushita, Uechi, Matsuzaki, Kenmochi, Takata, Sasaki, Ito, Ishibashi: "The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function." in: The Journal of biological chemistry, Vol. 286, Issue 5, pp. 3342-50, 2011 (PubMed).

Fuhs, Insel: "Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization." in: The Journal of biological chemistry, Vol. 286, Issue 17, pp. 14830-41, 2011 (PubMed).

van der Aa, Jouneau, Laplantine, Bouchez, Van Kemenade, Boudinot: "FinTRIMs, fish virus-inducible proteins with E3 ubiquitin ligase activity." in: Developmental and comparative immunology, Vol. 36, Issue 2, pp. 433-41, 2011 (PubMed).

van der Ven, Odgerel, Fürst, Goldfarb, Kono, Miyajima: "Dominant-negative effects of a novel mutation in the filamin myopathy." in: Neurology, Vol. 75, Issue 23, pp. 2137-8, 2010 (PubMed). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Park, Khanna, Rink, Gnyawali, Roy, Sen: "Glutathione disulfide induces neural cell death via a 12-lipoxygenase pathway." in: Cell death and differentiation, Vol. 16, Issue 8, pp. 1167-79, 2009 (PubMed).

Bratschi, Burrowes, Kulaga, Cheung, Alvarez, Kearley, Zaremberg: "Glycerol-3-phosphate acyltransferases gat1p and gat2p are microsomal phosphoproteins with differential contributions to polarized cell growth." in: Eukaryotic cell, Vol. 8, Issue 8, pp. 1184-96, 2009 (PubMed).

Kyosseva, Harris, Weigel: "The hyaluronan receptor for endocytosis mediates hyaluronan-dependent signal transduction via extracellular signal-regulated kinases." in: The Journal of biological chemistry, Vol. 283, Issue 22, pp. 15047-55, 2008 (PubMed).

Pandey, Harris, Weigel, Weigel: "The cytoplasmic domain of the hyaluronan receptor for endocytosis (HARE) contains multiple endocytic motifs targeting coated pit-mediated internalization." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21453-61, 2008 (PubMed).

Peterson, Phillips: "Characterization of conserved bases in 4.5S RNA of Escherichia coli by construction of new F' factors." in: Journal of bacteriology, Vol. 190, Issue 23, pp. 7709-18, 2008 (PubMed).

Chigira, Oka, Okajima, Jigami: "Engineering of a mammalian O-glycosylation pathway in the yeast Saccharomyces cerevisiae: production of O-fucosylated epidermal growth factor domains." in: Glycobiology, Vol. 18, Issue 4, pp. 303-14, 2008 (PubMed).

Asano, Watanabe, Kitani, Fuss, Strober: "Notch1 signaling and regulatory T cell function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 5, pp. 2796-804, 2008 (PubMed).

Harris, Kyosseva, Weigel, Weigel: "Expression, processing, and glycosaminoglycan binding activity of the recombinant human 315-kDa hyaluronic acid receptor for endocytosis (HARE)." in: The Journal of biological chemistry, Vol. 282, Issue 5, pp. 2785-97, 2007 (PubMed).

Wang, Su, Sun: "A novel ARID/Bright-like protein involved in transcriptional activation of cyst wall protein 1 gene in Giardia lamblia." in: The Journal of biological chemistry, Vol. 282, Issue 12, pp. 8905-14, 2007 (PubMed).

Nakamura, Weidinger, Liang, Aquilina-Beck, Tamai, Moon, Warman: "The CCN family member Wisp3, mutant in progressive pseudorheumatoid dysplasia, modulates BMP and Wnt signaling." in: The Journal of clinical investigation, Vol. 117, Issue 10, pp. 3075-86, 2007 (PubMed).

Carattino, Sheng, Bruns, Pilewski, Hughey, Kleyman: "The epithelial Na+ channel is inhibited by a peptide derived from proteolytic processing of its alpha subunit." in: The Journal of biological chemistry, Vol. 281, Issue 27, pp. 18901-7, 2006 (PubMed).

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