You are viewing an incomplete version of our website. Please click to reload the website as full version.

V5 Epitope Tag antibody

Details for Product No. ABIN153300, Supplier: Log in to see
Antigen
Reactivity
Tag
383
3
3
3
3
Host
Rabbit
221
127
35
8
3
Clonality
Polyclonal
Conjugate
Un-conjugated
28
27
19
10
9
9
8
8
8
8
8
8
8
8
8
7
7
6
5
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
333
246
100
77
61
53
50
26
13
10
8
8
4
3
3
3
2
2
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Rabbits were immunized with a synthetic peptide representing amino acid residues 95 to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH.
Purification Immunogen affinity purified
Alternative Name Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Target Type Tag
Research Area Tags/Labels
Application Notes Western Blot 1:1000-1:30000, ELISA 1:1000- 1:30000, Immunohistochemistry 1:100-1:400, Immunocytochemistry/Immunofluorescence 1:100-1:400, Immunoprecipitation 1 - 4 μg/mg lysateUse in Immunohistochemistry reported in scientific literature (PMID: 23785054). Use in Immunoprecipitation reported in scientific literature (PMID 26085104)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (ABIN153300) Western blot analysis of V5-tagged Nicastrin, using ABIN153300. Samples: NP-40 whole ...
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (ABIN153300) Western Blot: V5 Epitope Tag Antibody [ABIN153300] - 200, 100, or 50 ng of E. coli wh...
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (ABIN153300) Western Blot: V5 Epitope Tag Antibody [ABIN153300] - 200, 100, or 50 ng of E. coli wh...
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (ABIN153300) Western Blot: V5 Epitope Tag Antibody [ABIN153300] - Analysis using the HRP conjugate...
Immunofluorescence (IF) image for anti-V5 Epitope Tag antibody (ABIN153300) Immunofluorescence: V5 Epitope Tag Antibody [ABIN153300] - HEK293 cells transiently e...
Product cited in: Luo, Chen, Chen, Alkam, Wang, Semenza: "PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia." in: Oncotarget, Vol. 7, Issue 6, pp. 6379-97, 2016 (PubMed).

Diehl, Lin, Grubaugh, Carvalho, Kim, Kyawe, McCauley, Donnard, Kucukural, McDonel, Schaffner, Garber, Rambaut, Andersen, Sabeti, Luban: "Ebola Virus Glycoprotein with Increased Infectivity Dominated the 2013-2016 Epidemic." in: Cell, Vol. 167, Issue 4, pp. 1088-1098.e6, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Hale, Brittle, Fisher, Monk, Strutt: "Cellular interpretation of the long-range gradient of Four-jointed activity in the Drosophila wing." in: eLife, Vol. 4, 2015 (PubMed). (Sample species: Fruit Fly (Drosophila melanogaster)).

Butkinaree, Canuel, Essalmani, Poirier, Benjannet, Asselin, Roubtsova, Hamelin, Marcinkiewicz, Chamberland, Guillemot, Mayer, Sisodia, Jacob, Prat, Seidah: "Amyloid Precursor-like Protein 2 and Sortilin Do Not Regulate the PCSK9 Convertase-mediated Low Density Lipoprotein Receptor Degradation but Interact with Each Other." in: The Journal of biological chemistry, Vol. 290, Issue 30, pp. 18609-20, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Groth, Sasamura, Khanna, Whitley, Fortini: "Protein trafficking abnormalities in Drosophila tissues with impaired activity of the ZIP7 zinc transporter Catsup." in: Development (Cambridge, England), Vol. 140, Issue 14, pp. 3018-27, 2013 (PubMed). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Harder, Whiteman, Pieczynski, Liu, Margolis: "Snail destabilizes cell surface Crumbs3a." in: Traffic (Copenhagen, Denmark), Vol. 13, Issue 8, pp. 1170-85, 2012 (PubMed).

Thiaville, Stoeck, Chen, Wu, Magnani, Oidtman, Shih, Lupien, Wang: "Identification of PBX1 target genes in cancer cells by global mapping of PBX1 binding sites." in: PLoS ONE, Vol. 7, Issue 5, pp. e36054, 2012 (PubMed).

Chen, Honeyager, Schleede, Avanesov, Laughon, Blair: "Crossveinless d is a vitellogenin-like lipoprotein that binds BMPs and HSPGs, and is required for normal BMP signaling in the Drosophila wing." in: Development (Cambridge, England), Vol. 139, Issue 12, pp. 2170-6, 2012 (PubMed).

Tagliabracci, Engel, Wen, Wiley, Worby, Kinch, Xiao, Grishin, Dixon: "Secreted kinase phosphorylates extracellular proteins that regulate biomineralization." in: Science (New York, N.Y.), Vol. 336, Issue 6085, pp. 1150-3, 2012 (PubMed).

Haines, Vande Burgt, Francica, Kaletsky, Bates: "Chinese hamster ovary cell lines selected for resistance to ebolavirus glycoprotein mediated infection are defective for NPC1 expression." in: Virology, Vol. 432, Issue 1, pp. 20-8, 2012 (PubMed).

Ishikawa, Xu, Ogura, Manning, Irvine: "The Raine syndrome protein FAM20C is a Golgi kinase that phosphorylates bio-mineralization proteins." in: PLoS ONE, Vol. 7, Issue 8, pp. e42988, 2012 (PubMed).

Hance, Dole, Gopal, Bohonowych, Jezierska-Drutel, Neumann, Liu, Garraway, Isaacs: "Secreted Hsp90 is a novel regulator of the epithelial to mesenchymal transition (EMT) in prostate cancer." in: The Journal of biological chemistry, Vol. 287, Issue 45, pp. 37732-44, 2012 (PubMed). Further details: Western Blotting

Massinen, Hokkanen, Matsson, Tammimies, Tapia-Páez, Dahlström-Heuser, Kuja-Panula, Burghoorn, Jeppsson, Swoboda, Peyrard-Janvid, Toftgård, Castrén, Kere: "Increased expression of the dyslexia candidate gene DCDC2 affects length and signaling of primary cilia in neurons." in: PLoS ONE, Vol. 6, Issue 6, pp. e20580, 2011 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Xiao, Jin, Xue, Sorvillo, Voorberg, Zheng: "Essential domains of a disintegrin and metalloprotease with thrombospondin type 1 repeats-13 metalloprotease required for modulation of arterial thrombosis." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 10, pp. 2261-9, 2011 (PubMed). Further details: ELISA

Qazi, Halterman, Mi, Zhang, Schor: "Chopper is prodeath regardless of the effect of p75ICD on sensitivity to oxidative stress." in: Oxidative medicine and cellular longevity, Vol. 2011, pp. 391659, 2011 (PubMed).

Anguera, Ma, Clift, Namekawa, Kelleher, Lee: "Tsx produces a long noncoding RNA and has general functions in the germline, stem cells, and brain." in: PLoS genetics, Vol. 7, Issue 9, pp. e1002248, 2011 (PubMed).

Fu, Yucer, Liu, Li, Yi, Mu, Yang, Chu, Jung, OMalley, Gu, Qin, Wang: "RFWD3-Mdm2 ubiquitin ligase complex positively regulates p53 stability in response to DNA damage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 10, pp. 4579-84, 2010 (PubMed).

Hurd, Zhou, Jenkins, Liu, Swaroop, Khanna, Martens, Hildebrandt, Margolis: "The retinitis pigmentosa protein RP2 interacts with polycystin 2 and regulates cilia-mediated vertebrate development." in: Human molecular genetics, Vol. 19, Issue 22, pp. 4330-44, 2010 (PubMed).

Massinen, Tammimies, Tapia-Páez, Matsson, Hokkanen, Söderberg, Landegren, Castrén, Gustafsson, Treuter, Kere: "Functional interaction of DYX1C1 with estrogen receptors suggests involvement of hormonal pathways in dyslexia." in: Human molecular genetics, Vol. 18, Issue 15, pp. 2802-12, 2009 (PubMed).

Pétremand, Bulat, Butty, Poussin, Rütti, Au, Ghosh, Mooser, Thorens, Yang, Widmann, Waeber: "Involvement of 4E-BP1 in the protection induced by HDLs on pancreatic beta-cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 10, pp. 1572-86, 2009 (PubMed).