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V5 Epitope Tag antibody
| Antigen | V5 Epitope Tag |
| Clonality | Polyclonal |
| Host |
 Alternatives Rabbit |
| Reactivity | |
| Conjugate |
Alternatives Un-conjugated |
| Application |
Alternatives Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), ELISA |
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5 references available |
| Catalog no. | ABIN153300 |
| Quantity | 0.1 ml (1 mg/ml) (Variants) |
| Price | Product not available in this region. |
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Additional Information
| Alternative name | Pk (V5) Epitope Tag (GKPIPNPLLGLDST) |
| Immunogen | Rabbits were immunized with a synthetic peptide representing amino acid residues 95to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH. |
| Description | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteinsof the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9amino acid sequence (IPNPLLGLD). Alternate Names: anti-GKPIPNPLLGLDST epitope tag antibody, anti-GKPIPNPLLGLDST tag antibody,anti-V5 epitope tag antibody. |
| Specificity | Antibody recognizes the V5 cleavage site. Species Reactivity: All Species. |
Application Details
| Application Notes | Suggested working dilutions: ELISA 1:100-1:500 (Coating), 1:1000-1:30000 (Primary),Western Blot 1:1000-1:30000,Immunocytochemistry 1:100-1:400 |
| Concentration | 1 mg/ml |
| Purification | Aff - Purified |
| Buffer | Phosphate Buffered Saline. Preservative: 0.09% Sodium Azide. |
| Storage | Store at 4C. Do not freeze. |
| Research Area | Tags/Labels |
| Restrictions | For Research Use only |
Images
| Western blot analysis of V5-tagged Nicastrin, using ABIN153300. Samples: NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2). |
Publications
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Huang, Blagg, Keller et al.: "bZIP transcription factor interactions regulate DIF responses in Dictyostelium." in: Development (Cambridge, England), Vol. 133, Issue 3, pp. 449-58, 2006 (PubMed).
Mao, Rauskolb, Cho et al.: "Dachs: an unconventional myosin that functions downstream of Fat to regulate growth, affinity and gene expression in Drosophila." in: Development (Cambridge, England), Vol. 133, Issue 13, pp. 2539-51, 2006 (PubMed). Narita, Chien, Mullany et al.: "Loss of HSulf-1 expression enhances autocrine signaling mediated by amphiregulin in breast cancer." in: The Journal of biological chemistry, Vol. 282, Issue 19, pp. 14413-20, 2007 (PubMed). Bahary, Goishi, Stuckenholz et al.: "Duplicate VegfA genes and orthologues of the KDR receptor tyrosine kinase family mediate vascular development in the zebrafish." in: Blood, Vol. 110, Issue 10, pp. 3627-36, 2007 (PubMed). Liu, Vidensky, Ruggiero et al.: "Reticulon RTN2B regulates trafficking and function of neuronal glutamate transporter EAAC1." in: The Journal of biological chemistry, Vol. 283, Issue 10, pp. 6561-71, 2008 (PubMed). |
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Alternatives
Alternatives for antigen "V5 Epitope Tag", type "Antibodies"
| Hosts | Mouse (43), Rabbit (32), Goat (11), Chicken (6), Sheep (3) |
| Reactivities | Tag (17), tag (8), All Species (5), Virus (3), Paramyxovirus (1) |
| Applications | Western Blotting (WB) (76), ELISA (59), Immunohistochemistry (IHC) (23), Immunoprecipitation (IP) (21), Immunofluorescence (IF) (11), Immunocytochemistry (ICC) (10), Flow Cytometry (FACS) (7), Immunohistochemistry (Fixed) (IHC (fx)) (7), Cellular Assay (CA) (5), Dot Blot (Dot) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Radioimmunoassay (RIA) (3), Immunomicroscopy (IM) (2), Immunostaining (ISt) (1), Micro Array (1) |
| Conjugates | HRP (11), FITC (6), Biotin (5), Alkaline Phosphatase (AP) (2), APC (1), Agarose Beads (1), DyLight 488 (1), DyLight 550 (1), DyLight 650 (1), PerCP (1), SureLight® R-PE (1) |
| Epitopes | AA 95-108 (10), Tag (4) |
