V5 Epitope Tag antibody (HRP)

Details for Product No. ABIN153305, Supplier: Log in to see
Antigen
Reactivity
Tag
404
3
3
3
3
Host
Goat
221
135
36
9
5
4
3
1
1
Clonality
Polyclonal
Conjugate
HRP
28
27
19
11
10
10
9
9
9
8
8
8
8
8
8
7
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
342
265
122
92
71
64
53
36
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Options
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Immunogen Goats were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype IgG
Purification Immunogen affinity purified
Alternative Name Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Target Type Tag
Research Area Tags/Labels
Application Notes Western Blot 1:1000-1:10000, ELISA 1:10000 - 1:100000, Immunocytochemistry/Immunofluorescence 1:200-1:500Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 cyto 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot and immunocytochemistry.

Not for use in: Immunohistochemistry
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS and 0.2 % BSA
Buffer contains: 0.1 % Pro-Clean 400
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (HRP) (ABIN153305) Western Blot: V5 Epitope Tag Antibody [HRP] [ABIN153305] - 200, 100, or 50 ng of E. c...
Product cited in: Wang, Su, Sun: "A novel ARID/Bright-like protein involved in transcriptional activation of cyst wall protein 1 gene in Giardia lamblia." in: The Journal of biological chemistry, Vol. 282, Issue 12, pp. 8905-14, 2007 (PubMed).