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V5 Epitope Tag antibody (Agarose Beads)

Antigen

V5 Epitope Tag
Clonality Polyclonal
Host
Reactivity
Conjugate
Alternatives Agarose Beads
Application
Alternatives Immunoprecipitation (IP)
5 references available
Catalog no. ABIN153306
Quantity 0.1 mg
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Alternative name Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Immunogen Goat anti-V5 affinity purified Antibodies were coupled to agarose beads using acyanogen bromide method.
Description The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteinsof the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9amino acid sequence (IPNPLLGLD). Alternate Names: anti-GKPIPNPLLGLDST epitope tag antibody, anti-GKPIPNPLLGLDST tag antibody,anti-V5 epitope tag antibody.
Specificity Antibody recognizes the V5 cleavage site.

Application Details
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Application Notes Suggested working dilutions: IP 15 to 25 ul of gel slurry per 1 mg of protein lysate or extract.
Buffer 0.01 M Phosphate, pH 7.2, 0.1 M NaCl. Preservative: 0.09% Sodium Azide.
Storage Store at 4C. Do not freeze.
Research Area Tags/Labels
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Miller, Ahmad, Hague et al.: "L166P mutant DJ-1, causative for recessive Parkinson's disease, is degraded through the ubiquitin-proteasome system." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36588-95, 2003 (PubMed).

Hughey, Mueller, Bruns et al.: "Maturation of the epithelial Na+ channel involves proteolytic processing of the alpha- and gamma-subunits." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37073-82, 2003 (PubMed).

Zhang, Alfonta, Tian et al.: "Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 24, pp. 8882-7, 2004 (PubMed).

Hughey, Bruns, Kinlough et al.: "Distinct pools of epithelial sodium channels are expressed at the plasma membrane." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 48491-4, 2004 (PubMed).

Carattino, Sheng, Bruns et al.: "The epithelial Na+ channel is inhibited by a peptide derived from proteolytic processing of its alpha subunit." in: The Journal of biological chemistry, Vol. 281, Issue 27, pp. 18901-7, 2006 (PubMed).

Alternatives
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Alternatives for antigen "V5 Epitope Tag", type "Antibodies"
Hosts Rabbit (49), Mouse (43), Goat (11), Chicken (6), Sheep (3)
Reactivities Tag (35), tag (8), All Species (5), Virus (3), Paramyxovirus (1)
Applications Western Blotting (WB) (78), ELISA (61), Immunofluorescence (IF) (26), Immunohistochemistry (IHC) (23), Immunoprecipitation (IP) (22), Immunocytochemistry (ICC) (10), Flow Cytometry (FACS) (7), Immunohistochemistry (Fixed) (IHC (fx)) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Cellular Assay (CA) (5), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Radioimmunoassay (RIA) (3), Immunomicroscopy (IM) (2), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1), Micro Array (1)
Conjugates HRP (12), FITC (7), Biotin (6), Alkaline Phosphatase (AP) (2), APC (1), Agarose Beads (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), DyLight 488 (1), DyLight 550 (1), DyLight 650 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), PerCP (1), SureLight® R-PE (1)
Epitopes AA 95-108 (10), Tag (4)