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Details for Product No. ABIN153306

V5 Epitope Tag antibody (Agarose Beads)

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Clonality Polyclonal
»Alternatives Agarose Beads
»Alternatives Immunoprecipitation (IP)
Pubmed 5 references available
Catalog no. ABIN153306
Quantity 0.1 mg
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Immunogen Goat anti-V5 affinity purified Antibodies were coupled to agarose beads using acyanogen bromide method.
Specificity Antibody recognizes the V5 cleavage site.
Alternative Name Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteinsof the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9amino acid sequence (IPNPLLGLD). Alternate Names: anti-GKPIPNPLLGLDST epitope tag antibody, anti-GKPIPNPLLGLDST tag antibody,anti-V5 epitope tag antibody.
Research Area Tags/Labels
Application Notes Suggested working dilutions: IP 15 to 25 ul of gel slurry per 1 mg of protein lysate or extract.
Restrictions For Research Use only
Buffer 0.01 M Phosphate, pH 7.2, 0.1 M NaCl. Preservative: 0.09% Sodium Azide.
Preservative Sodium azide
Storage 4 °C
Miller, Ahmad, Hague et al.: "L166P mutant DJ-1, causative for recessive Parkinson's disease, is degraded through the ubiquitin-proteasome system." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36588-95, 2003 (PubMed).

Hughey, Mueller, Bruns et al.: "Maturation of the epithelial Na+ channel involves proteolytic processing of the alpha- and gamma-subunits." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37073-82, 2003 (PubMed).

Zhang, Alfonta, Tian et al.: "Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 24, pp. 8882-7, 2004 (PubMed).

Hughey, Bruns, Kinlough et al.: "Distinct pools of epithelial sodium channels are expressed at the plasma membrane." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 48491-4, 2004 (PubMed).

Carattino, Sheng, Bruns et al.: "The epithelial Na+ channel is inhibited by a peptide derived from proteolytic processing of its alpha subunit." in: The Journal of biological chemistry, Vol. 281, Issue 27, pp. 18901-7, 2006 (PubMed).

Alternatives for antigen "V5 Epitope Tag", type "Antibodies"
Hosts (86), (68), (18), (9), (1)
Reactivities (172), (5), (5), (1)
Applications (151), (117), (49), (43), (27), (25), (16), (11), (10), (9), (7), (7), (5), (5), (4), (2), (1)
Conjugates (23), (13), (9), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)