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V5 Epitope Tag (Cleavage Site) antibody (Agarose Beads)

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Cleavage Site
(9), (4)
(229), (4), (3), (3), (1)
(119), (92), (19), (6), (3)
Agarose Beads
(20), (18), (11), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunoprecipitation (IP)
(194), (129), (56), (53), (34), (26), (24), (11), (11), (9), (8), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Quantity 0.1 mg
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Immunogen Goats were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Goat anti-human V5 affinity purified antibodies were coupled to peptide immobilized on solid support.
Purification affinity purified
Alternative Name Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Target Type Tag
Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteinsof the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9amino acid sequence (IPNPLLGLD). Alternate Names: anti-GKPIPNPLLGLDST epitope tag antibody, anti-GKPIPNPLLGLDST tag antibody,anti-V5 epitope tag antibody.
Research Area Tags/Labels
Application Notes Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 cyto 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot and immunocytochemistry.
Recommended dilutions: Immunoprecipitation 1:10-1:500
Restrictions For Research Use only
Buffer Phosphate buffered Saline (PBS), Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Product cited in: Carattino, Sheng, Bruns et al.: "The epithelial Na+ channel is inhibited by a peptide derived from proteolytic processing of its alpha subunit." in: The Journal of biological chemistry, Vol. 281, Issue 27, pp. 18901-7, 2006 (PubMed).

Hughey, Bruns, Kinlough et al.: "Distinct pools of epithelial sodium channels are expressed at the plasma membrane." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 48491-4, 2004 (PubMed).

Zhang, Alfonta, Tian et al.: "Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 24, pp. 8882-7, 2004 (PubMed).

Hughey, Mueller, Bruns et al.: "Maturation of the epithelial Na+ channel involves proteolytic processing of the alpha- and gamma-subunits." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37073-82, 2003 (PubMed).

Miller, Ahmad, Hague et al.: "L166P mutant DJ-1, causative for recessive Parkinson's disease, is degraded through the ubiquitin-proteasome system." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36588-95, 2003 (PubMed).

Catalog No. ABIN153306
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