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Collagen, Type I (COL1) antibody

Details for Product No. ABIN153322, Supplier: Log in to see
Antigen
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
180
62
53
24
17
9
9
9
7
6
4
3
2
2
2
2
2
2
1
1
Host
Rabbit
183
54
39
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
30
7
5
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
181
98
95
85
78
67
65
43
26
25
22
15
14
8
5
4
4
2
1
1
1
Supplier
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Immunogen Collagen Type I from human and bovine placenta by limited pepsin digestion and selective salt precipitation.
Specificity Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Cross-Reactivity (Details) Expected to react with most mammalian Type I collagens.
Purification affinity purified
Alternative Name Collagen type I
Background Collagens are highly conserved throughout evolution and are characterized by anuninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helicalstructure. For these reasons it is often extremely difficult to generate antibodies withspecificities to collagens. The development of type specific antibodies is dependent onNON-DENATURED three-dimensional epitopes. This preparation results in a nativeconformation of the protein. Alternate Names: Anti-COL1A1 antibody, Anti-Collagen Of Skin Tendon And Bone antibody, Anti-CollagenType 1 antibody, Anti-OI4 antibody, Anti- Osteogenesis Imperfecta Type IV antibody,Anti-Pro alpha 1(I) collagen antibody.
Gene Symbol: COL1A1
Gene ID 1277, 29393
Research Area Extracellular Matrix
Pathways
Application Notes Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native PAGE western blotting for highly sensitive qualitative analysis. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for in immunoblotting. ICC/IF reported in the literature (PMID: 21903125) IHC-F reported in the literature (PMID: 22402320).
Recommended dilutions: ELISA 1:5000-1:50000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:50-1:200, Immunohistochemistry-Frozen 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunoprecipitation 1:100, Western Blot 1:1000-1:10000
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN153322) anti-Collagen, Type I (COL1) antibody
Product cited in: Keophiphath, Achard, Henegar et al.: "Macrophage-secreted factors promote a profibrotic phenotype in human preadipocytes." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 1, pp. 11-24, 2009 (PubMed).

She, Xiong, Hazra et al.: "Adipogenic transcriptional regulation of hepatic stellate cells." in: The Journal of biological chemistry, Vol. 280, Issue 6, pp. 4959-67, 2005 (PubMed).

Hazra, Xiong, Wang et al.: "Peroxisome proliferator-activated receptor gamma induces a phenotypic switch from activated to quiescent hepatic stellate cells." in: The Journal of biological chemistry, Vol. 279, Issue 12, pp. 11392-401, 2004 (PubMed).

Background publications Hashimoto, Jin, Liu et al.: "Bone marrow-derived progenitor cells in pulmonary fibrosis." in: The Journal of clinical investigation, Vol. 113, Issue 2, pp. 243-52, 2004 (PubMed).

Stefanovic, Schnabl, Brenner: "Inhibition of collagen alpha 1(I) expression by the 5' stem-loop as a molecular decoy." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 18229-37, 2002 (PubMed).