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Collagen, Type I (COL1) antibody

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Cow (Bovine), Human, Mammalian, Pig (Porcine), Rabbit, Rat (Rattus)
(189), (63), (54), (26), (18), (10), (9), (9), (7), (6), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1)
(194), (56), (41), (2), (1)
Clonality (Clone)
Monoclonal ()
(32), (7), (5), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA
(192), (105), (101), (86), (83), (71), (69), (43), (29), (28), (26), (15), (14), (8), (5), (4), (4), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Quantity 0.1 mL
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Immunogen Full length bovine native protein (purified).
Clone COL-1
Isotype IgG1
Specificity Recognizes the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
Cross-Reactivity (Details) Not yet tested in other species.
Purification Ascites
Alternative Name Collagen type I
Background The extracellular matrix is the material found in the extracellular environment of alltissues and organs. It consists of basement membranes and interstitial stroma. Thecomposition of the extracellular framework of all vertebrates is dominated by a class ofmolecules known as collagens,1 each with unique features suited for its function andlocation. The collagens are proteins composed of three subunit polypeptides that canvary in length, which interact to form a triple helix. The molecular basis of the triple helixis provided by a repeated unique amino acid sequence (Gly-x-y) and the polypeptidesgenerated are capable of assembly into fibrillar or other types of supramolecularassemblies which are deposited in the extracellular matrix. The number of distinctcollagen types presently recognized is more than fourteen. Type I collagen [. Alternate Names: Anti-COL1A1 antibody, Anti-Collagen Of Skin Tendon And Bone antibody, Anti-CollagenType 1 antibody, Anti-OI4 antibody, Anti-Osteogenesis Imperfecta Type IV antibody,Anti-Pro alpha 1(I) collagen antibody, anti-Collagen I.
Gene Symbol: COL1A1
Gene ID 1277, 29393
Research Area Extracellular Matrix
Application Notes The predicted molecular weight in western blot is 95 kDa. In immunohistochemical testing of acetone-fixed or unfixed frozen sections, strong staining of connective tissue fibers is seen. This antibody has been used in immunohistochemistry as reported in the literature (PMID: 22019120) Immunohistochemistry-Paraffin was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24242003)
Recommended dilutions: Dot Blot 1:100-1:2000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Frozen 1:2000, Immunohistochemistry-Paraffin
Protocol Protocol specific for Collagen I Antibody SampleHuman Tissue sections (Normal Skin and Basal Cell Carcinoma/Solar Elastos) ApplicationImmunohistochemistry (Formalin-fixed paraffin-embedded sections) Blocking step:NoneAntigen retrieval step:Heat mediatedIncubation time:30 minute(s)Dilution: 1/800Additional notesThe tissue was FFPE (formalin fixed, paraffin embedded tissue sectioned at 3-4 microns). The antigen retreival method was a heat-induced antigen retreival (HEIR) using a vegetable steamer. Sections were incubated in the steamer for 20 minutes in 1 mM EDTA buffer, pH 8.
0. Sections were then rinsed in H2O and TBS buffer prior to starting the staining protocol. The staining appeared to be very specific and robust, with very little to no non-specific background staining. Staining Summary &
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Background publications Gay, Fine: "Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry." in: Methods in enzymology, Vol. 145, pp. 148-67, 1987 (PubMed).

Werkmeister, Ramshaw: "Monoclonal antibodies to collagens for immunofluorescent examination of human skin." in: Acta dermato-venereologica, Vol. 69, Issue 5, pp. 399-402, 1989 (PubMed).

Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).

Catalog No. ABIN153357
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