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Collagen, Type I (COL1) antibody

Details for Product No. ABIN153357, Supplier: Log in to see
Antigen
Reactivity
Cow (Bovine), Horse (Equine), Human, Mammalian, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
195
82
73
31
16
15
11
9
9
8
7
6
6
6
4
3
3
2
2
2
2
2
2
Host
Mouse
186
59
38
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
31
7
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
188
100
93
91
82
79
70
42
29
27
19
15
14
8
7
3
3
2
1
1
1
1
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Immunogen Full length bovine native protein (purified).
Clone COL-1
Isotype IgG1
Specificity Recognizes the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
Purification Unpurified
Alternative Name Collagen type I
Background Gene Symbol: COL1A1
Molecular Weight Theoretical MW: 139 kDa
Gene ID 1277
Application Notes Western Blot 1:100-1:2000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:2000, Dot Blot 1:100-1:2000The predicted molecular weight in western blot is 95 kDa. In immunohistochemical testing of acetone-fixed or unfixed frozen sections, strong staining of connective tissue fibers is seen. This antibody has been used in IHC as reported in the literature (PMID: 22019120) including IHC-Paraffin (PMID 24242003) and ICC (PMID 27769912).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Collagen I Antibody SampleHuman Tissue sections (Normal Skin and Basal Cell Carcinoma/Solar Elastos) ApplicationImmunohistochemistry (Formalin-fixed paraffin-embedded sections) Blocking step:NoneAntigen retrieval step:Heat mediatedIncubation time:30 minute(s)Dilution: 1/800Additional notesThe tissue was FFPE (formalin fixed, paraffin embedded tissue sectioned at 3-4 microns). The antigen retreival method was a heat-induced antigen retreival (HEIR) using a vegetable steamer. Sections were incubated in the steamer for 20 minutes in 1 mM EDTA buffer, pH 8.
0. Sections were then rinsed in H2O and TBS buffer prior to starting the staining protocol. The staining appeared to be very specific and robust, with very little to no non-specific background staining. Staining Summary &
Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Collagen, Type I (COL1) antibody (ABIN153357) Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [ABIN153357] - Colla...
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN153357) Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [ABIN153357] - Analysis of ...
Western Blotting (WB) image for anti-Collagen, Type I (COL1) antibody (ABIN153357) Western Blot: Collagen I alpha 1 Antibody (COL-1) [ABIN153357] - Analysis of collagen...
Immunofluorescence (IF) image for anti-Collagen, Type I (COL1) antibody (ABIN153357) Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [ABIN153...
Immunohistochemistry (IHC) image for anti-Collagen, Type I (COL1) antibody (ABIN153357) Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [ABIN153357] - Analysis of ...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Collagen, Type I (COL1) antibody (ABIN153357) Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [ABIN153357] - taini...
Product cited in: Bono, Meghezi, Soncini, Piola, Mantovani, Fiore: "A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models." in: Annals of biomedical engineering, Vol. 45, Issue 6, pp. 1496-1510, 2017 (PubMed).

Cheng, Wahafu, Jiang, Liu, Qiao, Peng, Cheng, Zhang, He, Liu: "A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration." in: Scientific reports, Vol. 6, pp. 24134, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Sa, Xiong, Ren, Zhao, He: "Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models." in: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, Vol. 96, pp. 472-478, 2016 (PubMed).

Karti, Zengin, Cinar, Tutuncu, Karahan, Celik, Kucukerdonmez: "Effect of 1- and 6-Hour-Delayed Corneal Collagen Cross-Linking on Corneal Healing in a Rabbit Alkali-Burn Model: Clinical and Histological Observations." in: Cornea, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Otani, Komura, Komura, Ishimaru, Konishi, Komuro, Hoshi, Takato, Tabata, Iwanaka: "Optimal amount of basic fibroblast growth factor in gelatin sponges incorporating β-tricalcium phosphate with chondrocytes." in: Tissue engineering. Part A, Vol. 21, Issue 3-4, pp. 627-36, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Geburek, Lietzau, Beineke, Rohn, Stadler: "Effect of a single injection of autologous conditioned serum (ACS) on tendon healing in equine naturally occurring tendinopathies." in: Stem cell research & therapy, Vol. 6, pp. 126, 2015 (PubMed). (Sample species: Horse (Equine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

You, Fan, Duan, Tian, Dang, Wang, Wang: "Foxc2 over-expression in bone marrow mesenchymal stem cells stimulates osteogenic differentiation and inhibits adipogenic differentiation." in: Molecular and cellular biochemistry, Vol. 386, Issue 1-2, pp. 125-34, 2014 (PubMed). (Sample species: Rabbit). Further details: Immunocytochemistry,Immunofluorescence

Triulzi, Ratti, Tortoreto, Ghirelli, Aiello, Regondi, Di Modica, Cominetti, Carcangiu, Moliterni, Balsari, Casalini, Tagliabue: "Maspin influences response to doxorubicin by changing the tumor microenvironment organization." in: International journal of cancer, Vol. 134, Issue 12, pp. 2789-97, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Murali, Rai, Dombrowski, Lee, Lim, Bramono, Ling, Bell, Hinkley, Nathan, Hui, Wong, Nurcombe, Cool: "Affinity-selected heparan sulfate for bone repair." in: Biomaterials, Vol. 34, Issue 22, pp. 5594-605, 2013 (PubMed). (Sample species: Rabbit). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Reem, Kaminer-Israeli, Ruvinov, Cohen: "Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds." in: Biomaterials, Vol. 33, Issue 3, pp. 751-61, 2011 (PubMed).

Krasiński, Biskupski, Dzieciuchowicz, Kaczmarek, Krasińska, Staniszewski, Pawlaczyk, Stanisić, Majewski, Majewski: "The influence of elastic components of the venous wall on the biomechanical properties of different veins used for arterial reconstruction." in: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, Vol. 40, Issue 2, pp. 224-9, 2010 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Background publications Sanes, Engvall, Butkowski, Hunter: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).

Werkmeister, Ramshaw: "Monoclonal antibodies to collagens for immunofluorescent examination of human skin." in: Acta dermato-venereologica, Vol. 69, Issue 5, pp. 399-402, 1989 (PubMed).

Gay, Fine: "Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry." in: Methods in enzymology, Vol. 145, pp. 148-67, 1987 (PubMed).