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Collagen, Type I (COL1) antibody

Details for Product No. ABIN153357, Supplier: Log in to see
Antigen
Reactivity
Cow (Bovine), Horse (Equine), Human, Mammalian, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
178
60
51
23
17
9
9
8
7
6
4
2
2
2
2
2
2
2
1
1
Host
Mouse
181
54
39
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
30
7
5
3
3
3
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
181
98
95
85
78
67
65
43
26
25
22
15
12
8
5
4
4
2
1
1
1
Supplier
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Immunogen Full length bovine native protein (purified).
Clone COL-1
Isotype IgG1
Specificity Recognizes the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
Cross-Reactivity (Details) Not yet tested in other species.
Purification Ascites
Alternative Name Collagen type I
Background Gene Symbol: COL1A1
Molecular Weight Theoretical MW: 139 kDa
Application Notes Western Blot 1:100-1:2000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:2000, Dot Blot 1:100-1:2000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Collagen I Antibody SampleHuman Tissue sections (Normal Skin and Basal Cell Carcinoma/Solar Elastos) ApplicationImmunohistochemistry (Formalin-fixed paraffin-embedded sections) Blocking step:NoneAntigen retrieval step:Heat mediatedIncubation time:30 minute(s)Dilution: 1/800Additional notesThe tissue was FFPE (formalin fixed, paraffin embedded tissue sectioned at 3-4 microns). The antigen retreival method was a heat-induced antigen retreival (HEIR) using a vegetable steamer. Sections were incubated in the steamer for 20 minutes in 1 mM EDTA buffer, pH 8.
0. Sections were then rinsed in H2O and TBS buffer prior to starting the staining protocol. The staining appeared to be very specific and robust, with very little to no non-specific background staining. Staining Summary &
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background publications Sanes, Engvall, Butkowski, Hunter: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990

Werkmeister, Ramshaw: "Monoclonal antibodies to collagens for immunofluorescent examination of human skin." in: Acta dermato-venereologica, Vol. 69, Issue 5, pp. 399-402, 1989

Gay, Fine: "Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry." in: Methods in enzymology, Vol. 145, pp. 148-67, 1987