Actin, beta (ACTB) antibody

Antigen: Actin, beta (ACTB)

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Clonality: Monoclonal (AC-15)

Host: Mouse

Reactivity: Cow (Bovine), Dog (Canine), Chicken, Cat (Feline), Fish, Guinea Pig, Human, Leech, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN153386

Additional Information

Alternative name: Actin beta / ACTB

Gene ID: 60, 11461, 81822

Immunogen: Synthetic peptide conjugated to KLH, corresponding to amino acids 1-14 of Xenopuslaevis Actin (beta).

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Cat (Feline), Fish, Guinea Pig, Human, Mammalian, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)

Format: Ascites

Isotype: IgG1

Clone: AC-15

Description: Beta-actin is one of six different actin isoforms which have been identified. Actins arehighly conserved proteins that are involved in cell motility, structure, and integrity.Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently usedas a loading control for assays involving protein detection, such as Western blotting.Antibodies to beta-actin provide a specific and useful tool in studying the intracellulardistribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Alternate Names: Anti-A X actin like protein antibody, Anti-Actx antibody, Anti-Beta cytoskeletal actinantibody, Anti-Melanoma X actin antibody, Anti-PS1TP5BP1 antibody, Anti-Beta-actinantibody, Anti-Beta actin antibody, Anti-Actin, beta antibody.

Specificity: Species Reactivity: Cross-reacts with Cat, Chicken, Cow, Dog, Drosophila melanogaster, Fish (carp andgoldfish), Guinea Pig, Human, Leech, Mouse, Pig, Rabbit, Rat, and Sheep. Detectsbeta actin in a wide variety of tissues and species. Does not cross react with adultcardiac or skeletal muscle or amoeba beta actin. Not yet tested in other species.

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Application Details

Application Notes: Western Blot 1:5000,Immunocytochemistry 1:1000,Acetone Fixation. Positive Controls: Cultured human or chicken fibroblasts, as described in Liao et al.

Concentration: 2.8 mg/ml

Purification: Ascites

Buffer: Preservative: 15mm sodium azide

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Images

anti-Actin, beta (ACTB) antibody

anti-Actin, beta (ACTB) antibody (Image 2)

anti-Actin, beta (ACTB) antibody (Image 3)

Publications

North, Gimona, Cross et al.: "Calponin is localised in both the contractile apparatus and the cytoskeleton of smooth muscle cells." in: Journal of cell science, Vol. 107 ( Pt 3), pp. 437-44, 1994 (PubMed).

North, Gimona, Lando et al.: "Actin isoform compartments in chicken gizzard smooth muscle cells." in: Journal of cell science, Vol. 107 ( Pt 3), pp. 445-55, 1994 (PubMed).

Gimona, Vandekerckhove, Goethals et al.: "Beta-actin specific monoclonal antibody." in: Cell motility and the cytoskeleton, Vol. 27, Issue 2, pp. 108-16, 1994 (PubMed).

Liao, Xu, Wargovich: "Direct reprobing with anti-beta-actin antibody as an internal control for western blotting analysis." in: BioTechniques, Vol. 28, Issue 2, pp. 216-8, 2000 (PubMed).

Adler, Huang, Wolin et al.: "Oxidant stress leads to impaired regulation of renal cortical oxygen consumption by nitric oxide in the aging kidney." in: Journal of the American Society of Nephrology : JASN, Vol. 15, Issue 1, pp. 52-60, 2003 (PubMed).

Thornton, Kudo, Rayman et al.: "Degradation of NF-kappa B in T cells by gangliosides expressed on renal cell carcinomas." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 6, pp. 3480-90, 2004 (PubMed).

Hawkins, Fischer: "Inhibition of insulin synthesis by cyproheptadine: effects on translation." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 79, Issue 2, pp. 258-65, 2004 (PubMed).

Dietz, Souan, Knutson et al.: "Imatinib mesylate inhibits T-cell proliferation in vitro and delayed-type hypersensitivity in vivo." in: Blood, Vol. 104, Issue 4, pp. 1094-9, 2004 (PubMed).

Watters, Kraja, Meucci et al.: "Genome-wide discovery of loci influencing chemotherapy cytotoxicity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 32, pp. 11809-14, 2004 (PubMed).

Laughlin, McGugan, Powell et al.: "Involvement of raft-like plasma membrane domains of Entamoeba histolytica in pinocytosis and adhesion." in: Infection and immunity, Vol. 72, Issue 9, pp. 5349-57, 2004 (PubMed).

Mukherjee, Abate, Seyfried: "Antiangiogenic and proapoptotic effects of dietary restriction on experimental mouse and human brain tumors." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 16, pp. 5622-9, 2004 (PubMed).

Chong, Costain, Marriott et al.: "Differential display polymerase chain reaction reveals increased expression of striatal rat glia-derived nexin following chronic clozapine treatment." in: The pharmacogenomics journal, Vol. 4, Issue 6, pp. 379-87, 2004 (PubMed).

Adler, Huang: "Oxidant stress in kidneys of spontaneously hypertensive rats involves both oxidase overexpression and loss of extracellular superoxide dismutase." in: American journal of physiology. Renal physiology, Vol. 287, Issue 5, pp. F907-13, 2004 (PubMed).

Sung, Yang, Uryu et al.: "Modulation of nuclear factor-kappa B activity by indomethacin influences A beta levels but not A beta precursor protein metabolism in a model of Alzheimer's disease." in: The American journal of pathology, Vol. 165, Issue 6, pp. 2197-206, 2004 (PubMed).

Abramovich, Parborell, Tesone: "Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats." in: Biology of reproduction, Vol. 75, Issue 3, pp. 434-41, 2006 (PubMed).