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beta Actin antibody (Actin, beta) (N-Term)

Details for Product anti-ACTB Antibody No. ABIN153386, Supplier: Log in to see
Antigen
  • beta-actin
  • ps1tp5bp1
  • LOC563571
  • ACTB
  • A26C1A
  • A26C1B
  • act-2
  • actb
  • B-actin
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • BRWS1
  • PS1TP5BP1
  • ACT-5
  • Actx
  • E430023M04Rik
  • ACTA1
  • Beta-actin
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • POTE2alpha
  • POTEACTIN
  • actin, beta
  • similar to beta-cytoplasmic actin2
  • cytoplasmic actin OlCA1
  • actin beta
  • beta-actin
  • Actin 5C
  • actin, beta 1
  • actin, gamma 1
  • beta actin
  • POTE ankyrin domain family, member F
  • actb
  • LOC563571
  • ACTB
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • Act5C
  • Actb
  • actb1
  • ACTG1
  • LOC396797
  • LOC100350495
  • LOC100136352
  • POTEF
Epitope
N-Term
164
39
16
15
10
9
7
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Fish, Guinea Pig, Hamster, Human, Leech, Mammalian, Mouse (Murine), Pig (Porcine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine), Squirrel, Zebrafish (Danio rerio)
552
278
237
79
60
55
43
39
38
33
32
28
26
21
17
14
14
13
11
10
10
9
6
6
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
355
229
18
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This beta Actin antibody is un-conjugated
33
26
18
6
6
5
5
4
4
4
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
524
238
164
121
107
79
56
39
16
12
12
11
8
8
3
2
2
2
2
2
2
1
1
1
1
Options
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Immunogen slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Clone AC-15
Isotype IgG1
Specificity In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.
Cross-Reactivity (Details) Does not cross react with adult cardiac, skeletal muscle, drosophila,amoeba beta actin. Hamster reactivity reported in scientific literature (PMID: 24478435) Not yet tested in other species.
Purification Unpurified
Alternative Name beta-Actin (ACTB Antibody Abstract)
Background Gene Symbol: ACTB
Molecular Weight Theoretical MW: 42 kDa
Gene ID 60
Application Notes Western Blot 1:5000 - 1:10000, Simple Western 1:25, Flow Cytometry, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:500This antibody is useful for Western blot, ELISA, immunohistochemistry and Immunocytochemistry. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. This antibody is resistant to formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives. Use in immunoprecipitation reported in scientific literature (PMID 23647384). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size: Wes/Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Beta Actin Antibody IHC-FFPE sections:I. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) anti-Actin, beta (ACTB) (N-Term) antibody
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) anti-Actin, beta (ACTB) (N-Term) antibody (Image 2)
Immunofluorescence (IF) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) anti-Actin, beta (ACTB) (N-Term) antibody (Image 3)
Simple Western (SimWes) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Simple Western: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis using the HRP con...
Simple Western (SimWes) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Flow Cytometry: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis using the HRP con...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis of beta-actin in NI...
Flow Cytometry (FACS) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Flow Cytometry: beta-Actin Antibody (AC-15) [ABIN153386] - analysis of HeLa cells usi...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in Fib...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - MCDK cells induced with doxy...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in hum...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in CHO...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis of beta-Actin in bo...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in hum...
Immunofluorescence (IF) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [ABIN153386] - HS...
Immunohistochemistry (IHC) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153386) Immunohistochemistry: beta-Actin Antibody (AC-15) [ABIN153386] - Breast, Epithelium 40x.
Product cited in: Vannuvel, Van Steenbrugge, Demazy, Ninane, Fattaccioli, Fransolet, Renard, Raes, Arnould: "Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 1913-31, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Kral, Doriguzzi, Mayer, Krenbek, Setinek, Sutterlüty-Fall: "Differential Effects of Variations at Codon 106 on Sprouty2 Functions in Lung Cancer-Derived Cells." in: Journal of cellular biochemistry, Vol. 117, Issue 8, pp. 1822-32, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Cerqueira, Chausse, Baranovski, Liesa, Lewis, Shirihai, Kowaltowski: "Diluted serum from calorie-restricted animals promotes mitochondrial β-cell adaptations and protect against glucolipotoxicity." in: The FEBS journal, Vol. 283, Issue 5, pp. 822-33, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Davis, Collier, Leonardo, Seifert, Ajmo, Pennypacker: "Leukemia Inhibitory Factor Protects Neurons from Ischemic Damage via Upregulation of Superoxide Dismutase 3." in: Molecular neurobiology, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Westwell-Roper, Denroche, Ehses, Verchere: "Differential Activation of Innate Immune Pathways by Distinct Islet Amyloid Polypeptide (IAPP) Aggregates." in: The Journal of biological chemistry, Vol. 291, Issue 17, pp. 8908-17, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Maj, Hoermann, Rasul, Base, Wagner, Attems: "The Microtubule-Associated Protein Tau and Its Relevance for Pancreatic Beta Cells." in: Journal of diabetes research, Vol. 2016, pp. 1964634, 2016 (PubMed). Further details: Western Blotting

Yoon, Kleven, Paulsen, Kleppe, Wu, Ying, Gomez-Pinilla, Scarisbrick: "Interplay between exercise and dietary fat modulates myelinogenesis in the central nervous system." in: Biochimica et biophysica acta, Vol. 1862, Issue 4, pp. 545-55, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Andreev, Brosseau, Carmona, Mes-Masson, Ramotar: "The human organic cation transporter OCT1 mediates high affinity uptake of the anticancer drug daunorubicin." in: Scientific reports, Vol. 6, pp. 20508, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Naik, Dixit: "Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKCβ Kinase Activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 8, pp. 3438-51, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Acharya, Sokolovska, Tam, Conway, Stefani, Raso, Mukhopadhyay, Feliu, Paul, Savill, Hynes, Xavier, Vyas, Stuart, Lacy-Hulbert: "αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells." in: Nature communications, Vol. 7, pp. 10917, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Xu, Xu, Li, Jadhav, You, Yin, Zhang: "Carboxylesterase 1 Is Regulated by Hepatocyte Nuclear Factor 4α and Protects Against Alcohol- and MCD diet-induced Liver Injury." in: Scientific reports, Vol. 6, pp. 24277, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lai, Chen, Tsai, Chu, Yang, Chong, Chen: "Sexually Dimorphic Expression of eGFP Transgene in the Akr1A1 Locus of Mouse Liver Regulated by Sex Hormone-Related Epigenetic Remodeling." in: Scientific reports, Vol. 6, pp. 24023, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Radulovic, Yoon, Wu, Mustafa, Scarisbrick: "Targeting the thrombin receptor modulates inflammation and astrogliosis to improve recovery after spinal cord injury." in: Neurobiology of disease, Vol. 93, pp. 226-42, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Pastore, Brady, Diab, Martina, Sun, Huynh, Lim, Zare, Raben, Ballabio, Puertollano: "TFEB and TFE3 cooperate in the regulation of the innate immune response in activated macrophages." in: Autophagy, Vol. 12, Issue 8, pp. 1240-58, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Bhoopathi, Lee, Pradhan, Shen, Das, Sarkar, Emdad, Fisher: "mda-7/IL-24 Induces Cell Death in Neuroblastoma through a Novel Mechanism Involving AIF and ATM." in: Cancer research, Vol. 76, Issue 12, pp. 3572-82, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Tsai, Yeh, Tsai, Ting, Chiu, Tao, Li, Hung: "Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations." in: Journal of gastroenterology and hepatology, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Goettsch, Hutcheson, Hagita, Rogers, Creager, Pham, Choi, Mlynarchik, Pieper, Kjolby, Aikawa, Aikawa: "A single injection of gain-of-function mutant PCSK9 adeno-associated virus vector induces cardiovascular calcification in mice with no genetic modification." in: Atherosclerosis, Vol. 251, pp. 109-18, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Ekser, Mangus, Kubal, Fridell, Powelson, Nagaraju, Mihaylov, Phillips, Saxena, Goggins: "Recurrence of Hyperoxaluria and Kidney Disease after Combined Intestine-Kidney Transplantation for Enteric Hyperoxaluria." in: American journal of nephrology, Vol. 44, Issue 2, pp. 85-91, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Wong, Hristova, Ahlskog, Tasse, Ngsee, Chudalayandi, Bergeron: "Aberrant Subcellular Dynamics of Sigma-1 Receptor Mutants Underlying Neuromuscular Diseases." in: Molecular pharmacology, Vol. 90, Issue 3, pp. 238-53, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Sato, Ito, Nagasawa: "Regulatory effects of the L-lysine metabolites, L-2-aminoadipic acid and L-pipecolic acid, on protein turnover in C2C12 myotubes." in: Bioscience, biotechnology, and biochemistry, pp. 1-8, 2016 (PubMed).