beta Actin antibody (Actin, beta) (N-Term)

Details for Product anti-ACTB Antibody No. ABIN153386, Supplier: Log in to see
Antigen
  • beta-actin
  • ps1tp5bp1
  • LOC563571
  • ACTB
  • A26C1A
  • A26C1B
  • act-2
  • actb
  • B-actin
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • BRWS1
  • PS1TP5BP1
  • ACT-5
  • Actx
  • E430023M04Rik
  • ACTA1
  • Beta-actin
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • POTE2alpha
  • POTEACTIN
  • actin, beta
  • similar to beta-cytoplasmic actin2
  • cytoplasmic actin OlCA1
  • actin beta
  • beta-actin
  • Actin 5C
  • actin, beta 1
  • actin, gamma 1
  • beta actin
  • POTE ankyrin domain family, member F
  • actb
  • LOC563571
  • ACTB
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • Act5C
  • Actb
  • actb1
  • ACTG1
  • LOC396797
  • LOC100350495
  • LOC100136352
  • POTEF
Alternatives
anti-Cow (Bovine) beta Actin antibody for Immunocytochemistry
Epitope
N-Term
164
40
17
17
11
9
9
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Fish, Guinea Pig, Hamster, Human, Leech, Mammalian, Mouse (Murine), Pig (Porcine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine), Squirrel, Zebrafish
574
349
299
103
81
75
60
58
44
41
41
36
32
27
21
16
15
13
13
13
9
9
7
7
6
6
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
359
234
16
8
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This beta Actin antibody is un-conjugated
32
27
18
6
6
5
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
550
227
156
136
121
99
58
45
18
14
13
12
10
9
8
2
2
2
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Clone AC-15
Isotype IgG1
Specificity In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.
Purification Unpurified
Alternative Name beta-Actin (ACTB Antibody Abstract)
Background Gene Symbol: ACTB
Molecular Weight Theoretical MW: 42 kDa
Gene ID 60
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Application Notes Western Blot 1:5000 - 1:10000, Simple Western 1:25, Flow Cytometry, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:500This antibody is useful for Western blot, ELISA, immunohistochemistry and Immunocytochemistry. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. This antibody is resistant to formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives. Use in immunoprecipitation reported in scientific literature (PMID 23647384). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size: Wes/Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Beta Actin Antibody IHC-FFPE sections:I. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) anti-Actin, beta (ACTB) (N-Term) antibody
Western Blotting (WB) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) anti-Actin, beta (ACTB) (N-Term) antibody (Image 2)
Immunofluorescence (IF) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) anti-Actin, beta (ACTB) (N-Term) antibody (Image 3)
Simple Western (SimWes) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Simple Western: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis using the HRP con...
Simple Western (SimWes) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Flow Cytometry: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis using the HRP con...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis of beta-actin in NI...
Flow Cytometry (FACS) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Flow Cytometry: beta-Actin Antibody (AC-15) [ABIN153386] - analysis of HeLa cells usi...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in Fib...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - MCDK cells induced with doxy...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in hum...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in CHO...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis of beta-Actin in bo...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in hum...
Immunofluorescence (IF) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [ABIN153386] - HS...
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Immunohistochemistry: beta-Actin Antibody (AC-15) [ABIN153386] - Breast, Epithelium 40x.
Product cited in: Sansone, Galasso, Orefice, Nuzzo, Luongo, Cutignano, Romano, Brunet, Fontana, Esposito, Ianora: "The green microalga Tetraselmis suecica reduces oxidative stress and induces repairing mechanisms in human cells." in: Scientific reports, Vol. 7, pp. 41215, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Raats, Frenkel, van Schelven, Rinkes, Laoukili, Kranenburg: "CD95 ligand induces senescence in mismatch repair-deficient human colon cancer via chronic caspase-mediated induction of DNA damage." in: Cell death & disease, Vol. 8, Issue 3, pp. e2669, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Piccolo, Annunziata, Soria, Attanasio, Barbato, Castello, Carissimo, Quagliata, Terracciano, Brunetti-Pierri: "Down-regulation of hepatocyte nuclear factor-4α and defective zonation in livers expressing mutant Z α1-antitrypsin." in: Hepatology (Baltimore, Md.), 2017 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Chen, Hsieh, Li, Lin, Lai, Shieh, Chen, Young: "Study of poly-ɛ-caprolactone membranes for pleurodesis." in: Journal of the Formosan Medical Association = Taiwan yi zhi, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rabbit).

Wang, Boddicker, Dasari, Sidhu, Kadin, Macon, Ansell, Ketterling, Rech, Feldman: "Expression of p63 protein in anaplastic large cell lymphoma: Implications for genetic subtyping." in: Human pathology, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Giatromanolaki, Liousia, Arelaki, Kalamida, Pouliliou, Mitrakas, Tsolou, Sivridis, Koukourakis: "Differential effect of hypoxia and acidity on lung cancer cell and fibroblast metabolism." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 95, Issue 3, pp. 428-436, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Lee, Kim, Oh, Chung: "Fatty acid-binding protein 4 regulates fatty infiltration after rotator cuff tear by hypoxia-inducible factor 1 in mice." in: Journal of cachexia, sarcopenia and muscle, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Wang, Dasari, Nowakowski, Lazaridis, Wieben, Kadin, Feldman, Boddicker: "Retinoic acid receptor alpha drives cell cycle progression and is associated with increased sensitivity to retinoids in T-cell lymphoma." in: Oncotarget, Vol. 8, Issue 16, pp. 26245-26255, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Pettersson, Zarnegar, Westin, Persson, Peuckert, Jonsson, Hallgren, Kullander: "SLC10A4 regulates IgE-mediated mast cell degranulation in vitro and mast cell-mediated reactions in vivo." in: Scientific reports, Vol. 7, Issue 1, pp. 1085, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Balbaa, Abdulmalek, Khalil: "Oxidative stress and expression of insulin signaling proteins in the brain of diabetic rats: Role of Nigella sativa oil and antidiabetic drugs." in: PLoS ONE, Vol. 12, Issue 5, pp. e0172429, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Ambrož, Matoušková, Skarka, Zajdlová, Žáková, Skálová: "The Effects of Selected Sesquiterpenes from Myrica rubra Essential Oil on the Efficacy of Doxorubicin in Sensitive and Resistant Cancer Cell Lines." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Ho, Lee, Lu, Lane, Tsai, Ho, Huang, Chiang: "Effects of Electroacupuncture on Methamphetamine-Induced Behavioral Changes in Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 5642708, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Wu, Wang, Chen, Chao, Chang, Chen, Hung: "Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity." in: Stem cells translational medicine, Vol. 6, Issue 6, pp. 1504-1514, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Vannuvel, Van Steenbrugge, Demazy, Ninane, Fattaccioli, Fransolet, Renard, Raes, Arnould: "Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 1913-31, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Kral, Doriguzzi, Mayer, Krenbek, Setinek, Sutterlüty-Fall: "Differential Effects of Variations at Codon 106 on Sprouty2 Functions in Lung Cancer-Derived Cells." in: Journal of cellular biochemistry, Vol. 117, Issue 8, pp. 1822-32, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Cerqueira, Chausse, Baranovski, Liesa, Lewis, Shirihai, Kowaltowski: "Diluted serum from calorie-restricted animals promotes mitochondrial β-cell adaptations and protect against glucolipotoxicity." in: The FEBS journal, Vol. 283, Issue 5, pp. 822-33, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Davis, Collier, Leonardo, Seifert, Ajmo, Pennypacker: "Leukemia Inhibitory Factor Protects Neurons from Ischemic Damage via Upregulation of Superoxide Dismutase 3." in: Molecular neurobiology, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Westwell-Roper, Denroche, Ehses, Verchere: "Differential Activation of Innate Immune Pathways by Distinct Islet Amyloid Polypeptide (IAPP) Aggregates." in: The Journal of biological chemistry, Vol. 291, Issue 17, pp. 8908-17, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Maj, Hoermann, Rasul, Base, Wagner, Attems: "The Microtubule-Associated Protein Tau and Its Relevance for Pancreatic Beta Cells." in: Journal of diabetes research, Vol. 2016, pp. 1964634, 2016 (PubMed). Further details: Western Blotting

Yoon, Kleven, Paulsen, Kleppe, Wu, Ying, Gomez-Pinilla, Scarisbrick: "Interplay between exercise and dietary fat modulates myelinogenesis in the central nervous system." in: Biochimica et biophysica acta, Vol. 1862, Issue 4, pp. 545-55, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

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