GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase)

Details for Product anti-GAPDH Antibody No. ABIN153387, Supplier: Log in to see
Antigen
  • CG8893
  • Dmel\\CG8893
  • GA3PDH
  • GADPH
  • GAP
  • GAPDH
  • GAPDH II
  • GAPDH2
  • Gapd
  • Gapdh
  • Gapdh-2
  • Gapdh13F
  • GAPD
  • g3pd
  • gapd
  • GB14798
  • GAPC-2
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • T6J4.17
  • T6J4_17
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • BEST:GH12586
  • CG12055
  • Dmel\\CG12055
  • GAPDH I
  • GAPDH-1
  • GAPDH1
  • GAPDHI
  • Gapdh-1
  • Gapdh43E
  • gadph
  • gapdh
  • gapdh-1
  • gh12586
  • KNC-NDS6
  • G3PD
  • cb609
  • mg:bb02e05
  • wu:fb33a10
  • wu:ft80f05
  • G3PDH
  • Glyceraldehyde 3 phosphate dehydrogenase 2
  • glyceraldehyde-3-phosphate dehydrogenase
  • glyceraldehyde 3-phosphate dehydrogenase
  • similar to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
  • glyceraldehyde-3-phosphate dehydrogenase, type I
  • Glyceraldehyde 3 phosphate dehydrogenase 1
  • glyceraldehyde-3-phosphate dehydrogenase-like
  • olfactory receptor, family 8, subfamily K, member 3-like
  • Gapdh2
  • GAPDH
  • gapdh
  • Gapdh
  • GAPC2
  • gapDH
  • Gapdh1
  • LOC614985
Reactivity
Amphibian, Cat (Feline), Dog (Canine), Fish, Human, Monkey, Mouse (Murine), Pig (Porcine), Primate, Rabbit, Rat (Rattus)
742
425
341
160
133
83
78
60
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29
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18
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3
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1
1
1
1
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Host
Mouse
389
356
58
31
18
1
Clonality (Clone)
Monoclonal ()
Conjugate
This GAPDH antibody is un-conjugated
55
42
22
15
13
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11
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1
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1
Application
Immunoassay (IA), Immunocytochemistry (ICC), Immunofluorescence (IF), Simple Western (SimWes), Western Blotting (WB)
771
346
297
217
212
163
55
34
30
23
16
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11
7
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6
6
4
2
2
1
1
1
1
1
1
Options
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Immunogen Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human or rabbit GAPDH.
Clone 6C5
Isotype IgG1
No Cross-Reactivity Cow (Bovine), Goat
Purification Protein A purified
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background Gene Symbol: GAPDH
Molecular Weight Theoretical MW: 36 kDa
Gene ID 2597
UniProt P04406
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes Western Blot 0.5-1 μg/mL, Simple Western 1:100, Immunocytochemistry/Immunofluorescence 1:10-1:2000, ImmunoassaySince GAPDH is expressed in all cells it is for example becoming the marker of choice for a loading control in Western Blotting. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer PBS ( pH 7.4)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN153387) Western Blot: GAPDH Antibody (6C5) [ABIN153387] - Western blot analysis of GAPDH expr...
Simple Western (SimWes) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN153387) Simple Western: GAPDH Antibody (6C5) [ABIN153387] - Simple Western lane view shows a ...
Product cited in: Shao, Lee, Huang, Sheng, Henderson, Louvard, Sohn, Pepinsky, Mi: "LINGO-1 Regulates Oligodendrocyte Differentiation through the Cytoplasmic Gelsolin Signaling Pathway." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 12, pp. 3127-3137, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Jing, Duncan, Duncan: "A small-molecule screen reveals that HSP90β promotes the conversion of induced pluripotent stem cell-derived endoderm to a hepatic fate and regulates HNF4A turnover." in: Development (Cambridge, England), Vol. 144, Issue 10, pp. 1764-1774, 2017 (PubMed).

Ashton, Fokas, Kunz-Schughart, Folkes, Anbalagan, Huether, Kelly, Pirovano, Buffa, Hammond, Stratford, Muschel, Higgins, McKenna: "The anti-malarial atovaquone increases radiosensitivity by alleviating tumour hypoxia." in: Nature communications, Vol. 7, pp. 12308, 2016 (PubMed). Further details: Western Blotting

Cervantes-Gomez, Kumar Patel, Bose, Keating, Gandhi: "Decrease in total protein level of Bruton's tyrosine kinase during ibrutinib therapy in chronic lymphocytic leukemia lymphocytes." in: Leukemia, Vol. 30, Issue 8, pp. 1803-4, 2016 (PubMed).

Zhang, Tsuchiya, Kinoshita, Kushiyama, Suidasari, Hatakeyama, Imura, Kato, Suda: "Vitamin B6 Prevents IL-1β Protein Production by Inhibiting NLRP3 Inflammasome Activation." in: The Journal of biological chemistry, Vol. 291, Issue 47, pp. 24517-24527, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Baik, Jo, Seo, Ko, Cho, Lee, Lee: "Knockdown of RPL9 expression inhibits colorectal carcinoma growth via the inactivation of Id-1/NF-κB signaling axis." in: International journal of oncology, Vol. 49, Issue 5, pp. 1953-1962, 2016 (PubMed).

Giorgetti, Yu, Chua, Shimamura, Zhao, Zhu, Venneti, Pennuto, Guan, Hung, Lieberman: "Rescue of Metabolic Alterations in AR113Q Skeletal Muscle by Peripheral Androgen Receptor Gene Silencing." in: Cell reports, Vol. 17, Issue 1, pp. 125-36, 2016 (PubMed).

Tavori, Giunzioni, Predazzi, Plubell, Shivinsky, Miles, Devay, Liang, Rashid, Linton, Fazio: "Human PCSK9 promotes hepatic lipogenesis and atherosclerosis development via apoE- and LDLR-mediated mechanisms." in: Cardiovascular research, Vol. 110, Issue 2, pp. 268-78, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Leszczynska, Foskolou, Abraham, Anbalagan, Tellier, Haider, Span, ONeill, Buffa, Hammond: "Hypoxia-induced p53 modulates both apoptosis and radiosensitivity via AKT." in: The Journal of clinical investigation, Vol. 125, Issue 6, pp. 2385-98, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Han, Kim, Park, Park: "Hydrogen sulfide accelerates the recovery of kidney tubules after renal ischemia/reperfusion injury." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 30, Issue 9, pp. 1497-506, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Badie, Carlos, Folio, Okamoto, Bouwman, Jonkers, Tarsounas: "BRCA1 and CtIP promote alternative non-homologous end-joining at uncapped telomeres." in: The EMBO journal, Vol. 34, Issue 3, pp. 410-24, 2015 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

He, McCord, Hartnett, Aizenman: "Regulation of Pro-Apoptotic Phosphorylation of Kv2.1 K+ Channels." in: PLoS ONE, Vol. 10, Issue 6, pp. e0129498, 2015 (PubMed). (Sample species: Chinese Hamster). Further details: Western Blotting

Anbalagan, Biasoli, Leszczynska, Mukherjee, Hammond: "In Vitro Radiosensitization of Esophageal Cancer Cells with the Aminopeptidase Inhibitor CHR-2797." in: Radiation research, Vol. 184, Issue 3, pp. 259-65, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Zhuang, Chelluboina, Ponnala, Velpula, Rehman, Chetty, Zakharian, Rao, Veeravalli: "Involvement of nitric oxide synthase in matrix metalloproteinase-9- and/or urokinase plasminogen activator receptor-mediated glioma cell migration." in: BMC cancer, Vol. 13, pp. 590, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Savai, Al-Tamari, Sedding, Kojonazarov, Muecke, Teske, Capecchi, Weissmann, Grimminger, Seeger, Schermuly, Pullamsetti: "Pro-proliferative and inflammatory signaling converge on FoxO1 transcription factor in pulmonary hypertension." in: Nature medicine, Vol. 20, Issue 11, pp. 1289-300, 2014 (PubMed). Further details: Western Blotting

Håkansson, Lutay, Andersson, Hallgren, Westergren-Thorsson, Svensson, Godaly: "Epithelial G protein-coupled receptor kinases regulate the initial inflammatory response during mycobacterial infection." in: Immunobiology, Vol. 218, Issue 7, pp. 984-94, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Lin, Chien, Lin, Ke, Lin, Yeh: "An occult hepatitis B-derived hepatoma cell line carrying persistent nuclear viral DNA and permissive for exogenous hepatitis B virus infection." in: PLoS ONE, Vol. 8, Issue 5, pp. e65456, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Carlos, Escandell, Kotsantis, Suwaki, Bouwman, Badie, Folio, Benitez, Gomez-Lopez, Pisano, Jonkers, Tarsounas: "ARF triggers senescence in Brca2-deficient cells by altering the spectrum of p53 transcriptional targets." in: Nature communications, Vol. 4, pp. 2697, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Shanmugasundaram, Block, Nayak, Livi, Venkatachalam, Sudarshan: "PI3K regulation of the SKP-2/p27 axis through mTORC2." in: Oncogene, Vol. 32, Issue 16, pp. 2027-36, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Teng, Wu, Zhao, Yang, Wu, Wang: "Oxygen-sensitive mitochondrial accumulation of cystathionine ?-synthase mediated by Lon protease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 31, pp. 12679-84, 2013 (PubMed). Further details: Western Blotting