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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase)

Details for Product anti-GAPDH Antibody No. ABIN153387, Supplier: Log in to see
Antigen
  • CG8893
  • Dmel\\CG8893
  • GA3PDH
  • GADPH
  • GAP
  • GAPDH
  • GAPDH II
  • GAPDH2
  • Gapd
  • Gapdh
  • Gapdh-2
  • Gapdh13F
  • GAPD
  • g3pd
  • gapd
  • GB14798
  • GAPC-2
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • T6J4.17
  • T6J4_17
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • BEST:GH12586
  • CG12055
  • Dmel\\CG12055
  • GAPDH I
  • GAPDH-1
  • GAPDH1
  • GAPDHI
  • Gapdh-1
  • Gapdh43E
  • gadph
  • gapdh
  • gapdh-1
  • gh12586
  • KNC-NDS6
  • G3PD
  • cb609
  • mg:bb02e05
  • wu:fb33a10
  • wu:ft80f05
  • G3PDH
Reactivity
Dog (Canine), Chicken, Fish, Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis
543
220
193
111
56
51
44
31
29
25
18
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13
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8
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3
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2
1
1
1
1
1
1
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Host
Mouse
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229
58
31
20
Clonality (Clone)
Monoclonal ()
Conjugate
This GAPDH antibody is un-conjugated
42
39
18
6
6
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4
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2
2
2
1
1
1
1
1
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Application
Immunoassay (IA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
587
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147
127
86
55
44
26
21
17
10
9
7
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5
5
4
4
2
2
2
2
1
1
1
1
Supplier
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Immunogen Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human or rabbit GAPDH.
Clone 6C5
Isotype IgG1
No Cross-Reactivity Rabbit, Cow (Bovine), Goat
Cross-Reactivity (Details) Does not cross-react with bovine,goat. Not yet tested in other species.
Purification Protein A purified
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the keyenzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme incytoplasm, recent evidence suggests that mammalian GAPDH is also involved in agreat number of intracellular proceses such as membrane fusion, microtubule bundling,phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair.Recently data has implicated GAPDH in different pathologies including prostate cancerprogression, programmed neuronal cell death, age-related neuronal diseases, such asAlzheimer',s and Huntington',s disease. Some physiological factors, such ashypoxia and diabetes, increase GAPDH expression in certain cell types. Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.
Gene Symbol: GAPDH
Gene ID 2597, 14433, 24383
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Pathways
Application Notes Since GAPDH is expressed in all cells it is for example becoming the marker of choice for a loading control in Western Blotting.
Recommended dilutions: Western Blot 0.5-1 µg/mL, Immunoassay, Immunocytochemistry/Immunofluorescence 1:10-1:2000
Restrictions For Research Use only
Concentration 6.2 mg/mL
Buffer PBS. pH 7.4, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Product cited in: Kanadia, Shin, Yuan et al.: "Reversal of RNA missplicing and myotonia after muscleblind overexpression in a mouse poly(CUG) model for myotonic dystrophy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 31, pp. 11748-53, 2006 (PubMed).

Arthur, Akassoglou, Insel: "P2Y2 receptor activates nerve growth factor/TrkA signaling to enhance neuronal differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 52, pp. 19138-43, 2005 (PubMed).

He, Jazdzewski, Li et al.: "The role of microRNA genes in papillary thyroid carcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 52, pp. 19075-80, 2005 (PubMed).

Akassoglou, Malester, Xu et al.: "Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 14, pp. 5075-80, 2004 (PubMed).

Cuezva, Chen, Alonso et al.: "The bioenergetic signature of lung adenocarcinomas is a molecular marker of cancer diagnosis and prognosis." in: Carcinogenesis, Vol. 25, Issue 7, pp. 1157-63, 2004 (PubMed).

Sumner, Huynh, Markowitz et al.: "Valproic acid increases SMN levels in spinal muscular atrophy patient cells." in: Annals of neurology, Vol. 54, Issue 5, pp. 647-54, 2003 (PubMed).

Baskurt, Yalcin, Ozdem et al.: "Modulation of endothelial nitric oxide synthase expression by red blood cell aggregation." in: American journal of physiology. Heart and circulatory physiology, Vol. 286, Issue 1, pp. H222-9, 2003 (PubMed).

Irvin, Wood, Wang et al.: "TEL, a putative tumor suppressor, induces apoptosis and represses transcription of Bcl-XL." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46378-86, 2003 (PubMed).

Kapadia, Brideau-Andersen, Chisari: "Interference of hepatitis C virus RNA replication by short interfering RNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 4, pp. 2014-8, 2003 (PubMed).

Background publications Ikeda, Caldwell, Longnecker et al.: "Itchy, a Nedd4 ubiquitin ligase, downregulates latent membrane protein 2A activity in B-cell signaling." in: Journal of virology, Vol. 77, Issue 9, pp. 5529-34, 2003 (PubMed).