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beta Actin antibody (Actin, beta) (N-Term)

Details for Product anti-ACTB Antibody No. ABIN153388, Supplier: Login to see New
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(133), (38), (15), (14), (11), (7), (6), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Monkey, Mouse (Murine), Rat (Rattus)
(487), (190), (167), (52), (51), (40), (34), (32), (30), (23), (18), (14), (12), (8), (8), (7), (6), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
(314), (202), (19), (1), (1)
This beta Actin antibody is un-conjugated
(27), (19), (15), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Blocking Reagent (BR), Neutralization (Neut), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(461), (212), (146), (90), (85), (48), (42), (20), (12), (12), (11), (11), (9), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 15 references available
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Quantity 50 μL
Shipping to United States ( )
Immunogen A synthetic peptide made to an N-terminal region of human Beta Actin.
Cross-Reactivity (Details) Rabbit reactivity reported in the scientific literature (PMID: 23813946). Expected to cross-react with a wide range of species due to sequence homology.
Purification affinity purified
Alternative Name Actin beta / ACTB (ACTB Antibody Abstract)
Background Beta-actin is one of six different actin isoforms which have been identified. Actins arehighly conserved proteins that are involved in cell motility, structure, and integrity.Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently usedas a loading control for assays involving protein detection, such as Western blotting.Antibodies to beta-actin provide a specific and useful tool in studying the intracellulardistribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Alternate Names: Anti-A X actin like protein antibody, Anti-Actx antibody, Anti-Beta cytoskeletal actin,antibody, Anti-Melanoma X actin antibody, Anti-PS1TP5BP1 antibody, Anti-Beta-actinantibody, Anti-Beta actin antibody, Anti-Actin antibody, anti-beta antibody.
Gene Symbol: ACTB
Gene ID 60, 11461, 81822
UniProt P60709
Application Notes Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Immunocytochemistry/Immunofluorescence, Blocking/Neutralizing and Enzyme Immunoassay were reported in scientific literature. Customer review using ICC.
Recommended dilutions: Blocking/Neutralizing, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunohistochemistry 1:100, Immunohistochemistry-Paraffin 1:100, Immunoprecipitation 1:10-1:500, Western Blot 1:100-1:2000
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153388) Rabbit polyclonal to beta actin (ABIN153388) at 1/5000.
Product cited in: Martinsson-Ahlzuen, Liberal, Gruenenfelder et al.: "Cyclin-dependent kinase-associated proteins Cks1 and Cks2 are essential during early embryogenesis and for cell cycle progression in somatic cells." in: Molecular and cellular biology, Vol. 28, Issue 18, pp. 5698-709, 2008 (PubMed).

Zou, Chesler, Le Pichon et al.: "Absence of adenylyl cyclase 3 perturbs peripheral olfactory projections in mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 25, pp. 6675-83, 2007 (PubMed).

Park, Hallows, Chakrabarty et al.: "Conditional neuronal simian virus 40 T antigen expression induces Alzheimer-like tau and amyloid pathology in mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 11, pp. 2969-78, 2007 (PubMed).

Boutz, Chawla, Stoilov et al.: "MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development." in: Genes & development, Vol. 21, Issue 1, pp. 71-84, 2007 (PubMed).

Brault, Gobeil, Fortier et al.: "Lysophosphatidic acid induces endothelial cell death by modulating the redox environment." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 292, Issue 3, pp. R1174-83, 2007 (PubMed).

Garg, Barnes, Porgador et al.: "Vimentin expressed on Mycobacterium tuberculosis-infected human monocytes is involved in binding to the NKp46 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 9, pp. 6192-8, 2006 (PubMed).

Gobeil, Zhu, Brault et al.: "Nitric oxide signaling via nuclearized endothelial nitric-oxide synthase modulates expression of the immediate early genes iNOS and mPGES-1." in: The Journal of biological chemistry, Vol. 281, Issue 23, pp. 16058-67, 2006 (PubMed).

Wang, Hayashi, Serrero: "PC cell-derived growth factor confers resistance to dexamethasone and promotes tumorigenesis in human multiple myeloma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 1, pp. 49-56, 2006 (PubMed).

He, Li, Chang et al.: "GGA proteins mediate the recycling pathway of memapsin 2 (BACE)." in: The Journal of biological chemistry, Vol. 280, Issue 12, pp. 11696-703, 2005 (PubMed).

Sung, Yang, Uryu et al.: "Modulation of nuclear factor-kappa B activity by indomethacin influences A beta levels but not A beta precursor protein metabolism in a model of Alzheimer's disease." in: The American journal of pathology, Vol. 165, Issue 6, pp. 2197-206, 2004 (PubMed).

Adler, Huang: "Oxidant stress in kidneys of spontaneously hypertensive rats involves both oxidase overexpression and loss of extracellular superoxide dismutase." in: American journal of physiology. Renal physiology, Vol. 287, Issue 5, pp. F907-13, 2004 (PubMed).

Mukherjee, Abate, Seyfried: "Antiangiogenic and proapoptotic effects of dietary restriction on experimental mouse and human brain tumors." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 16, pp. 5622-9, 2004 (PubMed).

Watters, Kraja, Meucci et al.: "Genome-wide discovery of loci influencing chemotherapy cytotoxicity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 32, pp. 11809-14, 2004 (PubMed).

Adler, Huang, Wolin et al.: "Oxidant stress leads to impaired regulation of renal cortical oxygen consumption by nitric oxide in the aging kidney." in: Journal of the American Society of Nephrology : JASN, Vol. 15, Issue 1, pp. 52-60, 2003 (PubMed).

Witting, Maiorano, Davidson: "Ceramide enhances cholesterol efflux to apolipoprotein A-I by increasing the cell surface presence of ATP-binding cassette transporter A1." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 40121-7, 2003 (PubMed).

Catalog No. ABIN153388

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