beta Actin antibody (Actin, beta) (N-Term)

Details for Product anti-ACTB Antibody No. ABIN153388, Supplier: Log in to see
Antigen
  • beta-actin
  • ps1tp5bp1
  • LOC563571
  • ACTB
  • A26C1A
  • A26C1B
  • act-2
  • actb
  • B-actin
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • BRWS1
  • PS1TP5BP1
  • ACT-5
  • Actx
  • E430023M04Rik
  • ACTA1
  • Beta-actin
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • POTE2alpha
  • POTEACTIN
  • actin, beta
  • similar to beta-cytoplasmic actin2
  • cytoplasmic actin OlCA1
  • actin beta
  • beta-actin
  • Actin 5C
  • actin, beta 1
  • actin, gamma 1
  • beta actin
  • POTE ankyrin domain family, member F
  • actb
  • LOC563571
  • ACTB
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • Act5C
  • Actb
  • actb1
  • ACTG1
  • LOC396797
  • LOC100350495
  • LOC100136352
  • POTEF
Alternatives
anti-Rat (Rattus) beta Actin antibody for Blocking Peptide
Epitope
N-Term
162
40
17
17
11
9
9
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bird (Avian), Chinese Hamster, Fish, Human, Mouse (Murine), Pig (Porcine), Primate, Rabbit, Rat (Rattus)
570
346
298
102
81
75
59
58
45
42
41
37
31
28
21
16
16
13
13
12
9
9
8
7
7
6
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
354
233
16
8
1
1
Clonality
Polyclonal
Conjugate
This beta Actin antibody is un-conjugated
32
27
18
6
6
5
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Peptide (BP), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Proximity Ligation Assay (PLA), Simple Western (SimWes), Western Blotting (WB)
544
227
155
134
120
98
59
45
19
13
13
12
10
9
8
2
2
1
1
1
1
1
1
1
Options
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Immunogen A synthetic peptide made to an N-terminal region of human Beta Actin. [UniProt P60709].
Purification Immunogen affinity purified
Alternative Name beta-Actin (ACTB Antibody Abstract)
Background Gene Symbol: ACTB
Gene ID 60
UniProt P60709
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Application Notes Western Blot 1:100-1:2000, Simple Western 1:12.5, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Blocking/Neutralizing, Proximity Ligation Assay, Enzyme ImmunoassayPrior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. Immunocytochemistry/Immunofluorescence, Blocking/Neutralizing and Enzyme Immunoassay were reported in scientific literature. Customer review using ICC. Use in Proximity Ligation Assay reported in scientific literature (PMID 24740405). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153388) Rabbit polyclonal to beta actin (ABIN153388) at 1/5000.
Simple Western (SimWes) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153388) Simple Western: beta-Actin Antibody [ABIN153388] - Simple Western lane view shows a s...
Flow Cytometry (FACS) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153388) Flow Cytometry: beta-Actin Antibody [ABIN153388] - analysis of HeLa cells using mouse...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153388) Western Blot: beta-Actin Antibody [ABIN153388] - Western blot analysis of Jurkat, COS...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153388) Immunohistochemistry-Paraffin: beta-Actin Antibody [ABIN153388] - Analysis of Beta Ac...
Immunofluorescence (IF) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153388) Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [ABIN153388] - IF Confoca...
Product cited in: Molinuevo, Fernández, Cortizo, McCarthy, Schurman, Sedlinsky: "Advanced glycation end products and strontium ranelate promote osteogenic differentiation of vascular smooth muscle cells in vitro: Preventive role of vitamin D." in: Molecular and cellular endocrinology, Vol. 450, pp. 94-104, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Kramer, Wu, Crowe: "Tumor suppressor control of the cancer stem cell niche." in: Oncogene, Vol. 35, Issue 32, pp. 4165-78, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lee, Lee, Bae, Kang, Kim: "Genome-wide identification of target genes for miR-204 and miR-211 identifies their proliferation stimulatory role in breast cancer cells." in: Scientific reports, Vol. 6, pp. 25287, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Savas, Wei, Hsu, Falck, Guengerich, Capdevila, Johnson et al.: "20-Hydroxyeicosatetraenoic Acid (HETE)-dependent Hypertension in Human Cytochrome P450 (CYP) 4A11 Transgenic Mice: NORMALIZATION OF BLOOD PRESSURE BY SODIUM RESTRICTION, HYDROCHLOROTHIAZIDE, OR ..." in: The Journal of biological chemistry, Vol. 291, Issue 32, pp. 16904-19, 2016 (PubMed). (Sample species: Mouse (Murine)).

Douglas, Gardner, Salapa, Levin et al.: "Antibodies to the RNA Binding Protein Heterogeneous Nuclear Ribonucleoprotein A1 Colocalize to Stress Granules Resulting in Altered RNA and Protein Levels in a Model of Neurodegeneration in Multiple ..." in: Journal of clinical & cellular immunology, Vol. 7, Issue 2, pp. 402, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Lee, Nam, Jeong, Kim, Song, Pyeon, Kang, Hong, Nam, Joo: "Sensitive Tumorigenic Potential Evaluation of Adult Human Multipotent Neural Cells Immortalized by hTERT Gene Transduction." in: PLoS ONE, Vol. 11, Issue 7, pp. e0158639, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Pal, Mukhopadhyay, Ramaiah, Sarma, Bhadra, Bhadra: "Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells." in: PLoS ONE, Vol. 11, Issue 7, pp. e0159092, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Ciaraldi, Ryan, Mudaliar, Henry: "Altered Myokine Secretion Is an Intrinsic Property of Skeletal Muscle in Type 2 Diabetes." in: PLoS ONE, Vol. 11, Issue 7, pp. e0158209, 2016 (PubMed). Method employed by authors: Flow Cytometry (FACS)

Lange, Darrah, Vonderhaar, Mboko, Rekow, Patel, Sidjanin, Tarakanova: "Type I Interferon Counteracts Antiviral Effects of Statins in the Context of Gammaherpesvirus Infection." in: Journal of virology, Vol. 90, Issue 7, pp. 3342-54, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Rabbit).

Hu, Qian, Müller, Xu, Saba, Kim, Chen, Jiang, Wang, Zhang, Lane, Hoyt, Shin, Chen: "Biomarker quantification by multiplexed quantum dot technology for predicting lymph node metastasis and prognosis in head and neck cancer." in: Oncotarget, Vol. 7, Issue 28, pp. 44676-44685, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Palmer, Kimberly deRonde, Brown-Steinke, Gunter, Jyothikumar, Forbes, Lewis: "Hypoxia-induced changes in protein s-nitrosylation in female mouse brainstem." in: American journal of respiratory cell and molecular biology, Vol. 52, Issue 1, pp. 37-45, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Hsu, Konanur, Taing, Usui, Kayser, Goran, Kanoski: "Effects of sucrose and high fructose corn syrup consumption on spatial memory function and hippocampal neuroinflammation in adolescent rats." in: Hippocampus, Vol. 25, Issue 2, pp. 227-39, 2015 (PubMed). Further details: Western Blotting

Golden, Cantley: "Casein kinase 2 prevents mesenchymal transformation by maintaining Foxc2 in the cytoplasm." in: Oncogene, Vol. 34, Issue 36, pp. 4702-12, 2015 (PubMed). Further details: Western Blotting

Rahman, Thomas: "Molecular characterization and hypoxia-induced upregulation of neuronal nitric oxide synthase in Atlantic croaker: Reversal by antioxidant and estrogen treatments." in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol. 185, pp. 91-106, 2015 (PubMed). (Sample species: Fish). Further details: Western Blotting

Zhang, Pang, Tang, Yin, Wang: "Overexpression of hypoxia-inducible factor prolyl hydoxylase-2 attenuates hypoxia-induced vascular endothelial growth factor expression in luteal cells." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 3809-14, 2015 (PubMed).

Wang, Wu, Tan, Fei, Deng, Cao, Chen, Wang, Magdalou, Chen: "Cytotoxic effects of the quinolone levofloxacin on rabbit meniscus cells." in: Journal of applied toxicology : JAT, Vol. 34, Issue 8, pp. 870-7, 2014 (PubMed). (Sample species: Rabbit). Further details: Western Blotting

Bielinska, Makidon, Janczak, Blanco, Swanson, Smith, Pham, Szabo, Kukowska-Latallo, Baker: "Distinct pathways of humoral and cellular immunity induced with the mucosal administration of a nanoemulsion adjuvant." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 6, pp. 2722-33, 2014 (PubMed). Further details: Western Blotting

Calabrese, Saffin, Halpain: "Activity-dependent dendritic spine shrinkage and growth involve downregulation of cofilin via distinct mechanisms." in: PLoS ONE, Vol. 9, Issue 4, pp. e94787, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Proximity Ligation Assay

Holden, Lenio, Kuick, Ramakrishnan, Shah, Bachman: "Bacterial siderophores that evade or overwhelm lipocalin 2 induce hypoxia inducible factor 1α and proinflammatory cytokine secretion in cultured respiratory epithelial cells." in: Infection and immunity, Vol. 82, Issue 9, pp. 3826-36, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Wang, Kuo: "2,4,5-trimethoxybenzaldehyde, a bitter principle in plants, suppresses adipogenesis through the regulation of ERK1." in: Journal of agricultural and food chemistry, Vol. 62, Issue 40, pp. 9860-7, 2014 (PubMed).