anti-Actin antibody (AA 359-368)

Details for Product anti-Actin Antibody No. ABIN153390
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Antigen
Synonyms
Act57, Act57A, Act57b, Actin, BEST:RE38067, C, CG10067, CG30294, Dmel\\CG10067, M, T10, act 57A, act57A, act57B, act57b, actin, anon-EST:CL2c12, 143060_f_at, CG7478, D, Dmel\\CG7478, act 79B, act79B,  ... show more
Act57, Act57A, Act57b, Actin, BEST:RE38067, C, CG10067, CG30294, Dmel\\CG10067, M, T10, act 57A, act57A, act57B, act57b, actin, anon-EST:CL2c12, 143060_f_at, CG7478, D, Dmel\\CG7478, act 79B, act79B, mus79B, Act(88F), Act88-F, Act88f, CG5178, Dmel\\CG5178, E, Ifm(3)7, act 88F, act88F, rsd, Act87e, CG18290, Dmel\\CG18290, act 87E, act87E, mus87E, Act88F, Dsim\\GD19025, GD19025, dsim_GLEANR_2829, 42A, A, AFFX-Dros-ACTIN_M_r_at, Act, Act42, Act42a, Actin/BAP47, BAP47, CG12051, Dmel\\CG12051, act 42A, act42A, cytoplasmic show less
Epitope
AA 359-368
(37), (24), (13), (12), (8), (7), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Guinea Pig, Goat, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(141), (74), (69), (49), (13), (10), (8), (7), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(100), (99), (5), (2)
Clonality
Polyclonal
Conjugate
This Actin antibody is un-conjugated
(9), (6), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(155), (70), (59), (54), (52), (32), (30), (19), (10), (4), (3), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Immunogen Synthetic peptide: KQEYDESGPSC conjugated to KLH, corresponding to amino acids 359-368 of Human beta Actin.
Cross-Reactivity (Details) Expected to cross-react with a wide range of species including most vertebrates,some yeast due to sequence identity.
Purification affinity purified
Alternative Name Actin (pan)
Background In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have beenidentified. The alpha actins are found in muscle tissues and are a major constituent ofthe contractile apparatus. The beta and gamma actins coexist in most cell types ascomponents of the cyto-skeleton and as mediators of internal cell motility. Beta actinsare highly conserved proteins that are involved in cell motility, structure and integrity.Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells.Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in theform of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as anexcellent loading control. Alternate Names: anti-ActG antibody, anti-ActG1 antibody, anti-Actin antibody, anti-Gamma1 antibody,anti-DFNA20 antibody.
Gene Symbol: ACTB
Gene ID 60, 11461
Research Area Extracellular Matrix
Application Notes This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding - beta actin by western blotting in the appropriate cell lysate or extract.
Recommended dilutions: ELISA 1:10000-1:40000, Immunocytochemistry/Immunofluorescence 1:500-1:2000, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:10-1:2000, Western Blot 1:1000-1:4000
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Actin (AA 359-368) antibody Detection of actin using ABIN153390. Lane 1: HeLa cell lysate, Lane 2: rat brain, Lan...
anti-Actin (AA 359-368) antibody (2) Figure 1. Western blot using anti-beta Actin antibody shows detection of a predomina...
anti-Actin (AA 359-368) antibody (3) Figure 2. Immunohistochemical staining using Affinity Purified anti-beta Actin antibo...
General Lee, Bone, Strege et al.: "VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia." in: Blood, Vol. 104, Issue 3, pp. 788-94, 2004 (PubMed).

Validation Images
Catalog No. ABIN153390
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