Actin (AA 359-368) antibody

Details for Product No. ABIN153390
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Antigen
Synonyms
Act57, Act57A, Act57b, Actin, BEST:RE38067, C, CG10067, CG30294, Dmel\\CG10067, M, T10, act 57A, act57A, act57B, act57b, actin, anon-EST:CL2c12, 143060_f_at, CG7478, D, Dmel\\CG7478, act 79B, act79B,  ... show more
Act57, Act57A, Act57b, Actin, BEST:RE38067, C, CG10067, CG30294, Dmel\\CG10067, M, T10, act 57A, act57A, act57B, act57b, actin, anon-EST:CL2c12, 143060_f_at, CG7478, D, Dmel\\CG7478, act 79B, act79B, mus79B, Act(88F), Act88-F, Act88f, CG5178, Dmel\\CG5178, E, Ifm(3)7, act 88F, act88F, rsd, Act87e, CG18290, Dmel\\CG18290, act 87E, act87E, mus87E, Act88F, Dsim\\GD19025, GD19025, dsim_GLEANR_2829, 42A, A, AFFX-Dros-ACTIN_M_r_at, Act, Act42, Act42a, Actin/BAP47, BAP47, CG12051, Dmel\\CG12051, act 42A, act42A show less
Epitope
AA 359-368
(17), (8), (5), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Guinea Pig, Goat, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(107), (64), (64), (43), (20), (15), (15), (13), (8), (7), (6), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(93), (57), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(106), (49), (47), (37), (29), (25), (20), (18), (13), (2), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN153390
Quantity 0.1 mg
Price
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Immunogen Synthetic peptide: KQEYDESGPSC conjugated to KLH, corresponding to amino acids 359-368 of Human beta Actin.
Cross-Reactivity (Details) Expected to cross-react with a wide range of species including most vertebrates,some yeast due to sequence identity.
Purification affinity purified
Alternative Name Actin (pan)
Background In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have beenidentified. The alpha actins are found in muscle tissues and are a major constituent ofthe contractile apparatus. The beta and gamma actins coexist in most cell types ascomponents of the cyto-skeleton and as mediators of internal cell motility. Beta actinsare highly conserved proteins that are involved in cell motility, structure and integrity.Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells.Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in theform of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as anexcellent loading control. Alternate Names: anti-ActG antibody, anti-ActG1 antibody, anti-Actin antibody, anti-Gamma1 antibody,anti-DFNA20 antibody.
Gene Symbol: ACTB
Gene ID 60, 11461
Research Area Extracellular Matrix
Application Notes This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding - beta actin by western blotting in the appropriate cell lysate or extract.
Recommended dilutions: ELISA 1:10000-1:40000, Immunocytochemistry/Immunofluorescence 1:500-1:2000, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:10-1:2000, Western Blot 1:1000-1:4000
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Actin (AA 359-368) antibody Detection of actin using ABIN153390. Lane 1: HeLa cell lysate, Lane 2: rat brain, Lane 3: mouse brain, Lane 4: 3T3 cell lysate.
anti-Actin (AA 359-368) antibody (2) Figure 1. Western blot using anti-beta Actin antibody shows detection of a predominant band at ~42 kDa corresponding to beta Actin (arrowhead) in various whole cell lysates. Lysates are as follows: human embryonic kidney 293 (lane 2), human lung carcinoma A549 (lane 3) and mouse brain (lane 4) all using the 800 nm channel (green). ~ 35 ?g of each lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4? C followed by washes and reaction with a 1:10,000 dilution of IRDye?800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Molecular weight markers are shown in lane 1 using the 700 nm channel (red). IRDye?800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
anti-Actin (AA 359-368) antibody (3) Figure 2. Immunohistochemical staining using Affinity Purified anti-beta Actin antibody at 1:200 shows detection of actin at the neuromuscular juntion of rana pipiens tissue fixed in 4% paraformaldehyde using a section 4.2 ?m thick. Personal Communication. S. Moeckel-Cole, Univ. MA, Amherst, MA.
General Lee, Bone, Strege et al.: "VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia." in: Blood, Vol. 104, Issue 3, pp. 788-94, 2004 (PubMed).

Hosts (93), (57), (2), (1)
Reactivities (107), (64), (64), (43), (20), (15), (15), (13), (8), (7), (6), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (106), (49), (47), (37), (29), (25), (20), (18), (13), (2), (1), (1)
Conjugates (9), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Epitopes (17), (8), (5), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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