beta Actin antibody (Actin, beta)

Details for Product anti-ACTB Antibody No. ABIN153390, Supplier: Log in to see
Antigen
  • beta-actin
  • ps1tp5bp1
  • LOC563571
  • ACTB
  • A26C1A
  • A26C1B
  • act-2
  • actb
  • B-actin
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • BRWS1
  • PS1TP5BP1
  • ACT-5
  • Actx
  • E430023M04Rik
  • ACTA1
  • Beta-actin
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • POTE2alpha
  • POTEACTIN
  • actin, beta
  • similar to beta-cytoplasmic actin2
  • cytoplasmic actin OlCA1
  • actin beta
  • beta-actin
  • Actin 5C
  • actin, beta 1
  • actin, gamma 1
  • beta actin
  • POTE ankyrin domain family, member F
  • actb
  • LOC563571
  • ACTB
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • Act5C
  • Actb
  • actb1
  • ACTG1
  • LOC396797
  • LOC100350495
  • LOC100136352
  • POTEF
Alternatives
anti-Human beta Actin antibody for Immunofluorescence (fixed cells)
Reactivity
Cow (Bovine), Goat, Guinea Pig, Hamster, Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus), Sheep (Ovine)
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344
295
101
80
73
59
56
44
41
40
36
32
26
22
15
15
13
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12
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4
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3
2
2
2
1
1
1
1
1
1
1
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1
Host
Rabbit
345
224
16
8
1
1
Clonality
Polyclonal
Conjugate
This beta Actin antibody is un-conjugated
32
27
18
6
6
5
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2
2
2
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1
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1
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1
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1
1
1
1
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1
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1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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96
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45
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12
10
9
8
2
2
2
2
2
1
1
1
1
Options
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Immunogen Synthetic peptide: KQEYDESGPSC conjugated to KLH, corresponding to amino acids 359-368 of Human beta Actin.
Purification Immunogen affinity purified
Alternative Name beta-Actin (ACTB Antibody Abstract)
Background Gene Symbol: ACTB
Molecular Weight Theoretical MW: 42 kDa
Gene ID 60
UniProt P60709
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Application Notes Western Blot 1:1000-1:4000, ELISA 1:10000-1:40000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:500-1:2000, Immunohistochemistry-Paraffin 1:10-1:500This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding - beta actin by western blotting in the appropriate cell lysate or extract. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.3 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (ABIN153390) Detection of actin using ABIN153390. Lane 1: HeLa cell lysate, Lane 2: rat brain, Lan...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (ABIN153390) Figure 1. Western blot using anti-beta Actin antibody shows detection of a predomina...
Immunofluorescence (IF) image for anti-beta Actin antibody (Actin, beta) (ABIN153390) Figure 2. Immunohistochemical staining using Affinity Purified anti-beta Actin antibo...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (ABIN153390) Western Blot: beta-Actin Antibody [ABIN153390] - analysis of Beta Actin on HeLa and L...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (ABIN153390) Western Blot: beta-Actin Antibody [ABIN153390] - Lane 1: molecular weight. Lane 2: hu...
Product cited in: Gu, Helenius, Väänänen, Bulanova, Saarela, Sokolenko, Martens, Imyanitov, Kuznetsov: "BRCA1-deficient breast cancer cell lines are resistant to MEK inhibitors and show distinct sensitivities to 6-thioguanine." in: Scientific reports, Vol. 6, pp. 28217, 2016 (PubMed). Further details: Western Blotting

Bigot, Colli, Machiela, Jessop, Myers, Carrouget, Wagner, Roberson, Eymerit, Henrion, Chanock: "Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41." in: Nature communications, Vol. 7, pp. 12098, 2016 (PubMed). Further details: Western Blotting

Gerrits, Paradé, Koonen-Reemst, Bakker, Timmer-Hellings, Sollewijn Gelpke, Gossen: "Reversible infertility in a liver receptor homologue-1 (LRH-1)-knockdown mouse model." in: Reproduction, fertility, and development, Vol. 26, Issue 2, pp. 293-306, 2014 (PubMed). (Sample species: Mouse (Murine)).

Chang, Patel, Gwede, Morgado, Tomasevich, Fong, Farach-Carson, Delk: "IL-1? induces p62/SQSTM1 and represses androgen receptor expression in prostate cancer cells." in: Journal of cellular biochemistry, Vol. 115, Issue 12, pp. 2188-97, 2014 (PubMed).

Gu, Bouwman, Greco, Saarela, Yadav, Jonkers, Kuznetsov: "Suppression of BRCA1 sensitizes cells to proteasome inhibitors." in: Cell death & disease, Vol. 5, pp. e1580, 2014 (PubMed).

Ozkucur, Perike, Sharma, Funk: "Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness." in: BMC cell biology, Vol. 12, pp. 4, 2011 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Denis, Palmer-Smith, Elisma, Busuttil, Wright, Bou Khalil, Prat, Seidah, Chrétien, Mayne, Figeys: "Quantitative proteomic analysis of PCSK9 gain of function in human hepatic HuH7 cells." in: Journal of proteome research, Vol. 10, Issue 4, pp. 2011-26, 2011 (PubMed).

Mitchell, Lau, Lambert, Zhou, Fong, Couture, Figeys, Baetz: "Regulation of septin dynamics by the Saccharomyces cerevisiae lysine acetyltransferase NuA4." in: PLoS ONE, Vol. 6, Issue 10, pp. e25336, 2011 (PubMed).

Young, Guttman, Vaid, Vogl: "Tubulobulbar complexes are intercellular podosome-like structures that internalize intact intercellular junctions during epithelial remodeling events in the rat testis." in: Biology of reproduction, Vol. 80, Issue 1, pp. 162-74, 2008 (PubMed). Further details: Immunocytochemistry,Immunoelectron Microscopy,Immunofluorescence

Lee, Bone, Strege, Shanafelt, Jelinek, Kay: "VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia." in: Blood, Vol. 104, Issue 3, pp. 788-94, 2004 (PubMed).