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beta Actin antibody (Actin, beta)

Details for Product anti-ACTB Antibody No. ABIN153390, Supplier: Log in to see
Antigen
  • A
  • A26C1A
  • A26C1B
  • Ac5C
  • Act
  • act
  • act-2
  • ACT-5
  • Act-5C
  • Act5
  • act 5C
  • ACT5C
  • Act5c
  • act5C
  • ACTA1
  • ACTB
  • actb
  • actba
  • actin
  • Actin
  • Actin/BAP47
  • actin5C
  • Actin5C
  • Actx
  • anon-EST:fe2D2
  • B-actin
  • B-ACTZF
  • Bact
  • bact
  • bactin1
  • bactzf
  • BAP47
  • Bap47
  • beta-actin
  • Beta-actin
  • beta-actin/Bap47
  • BRWS1
  • CG4027
  • cyt5C
  • Dmel\\CG4027
  • E430023M04Rik
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • LOC563571
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • M32055
  • POTE2alpha
  • POTEACTIN
  • ps1tp5bp1
  • PS1TP5BP1
  • T11
  • wu:fd18f05
Reactivity
Cow (Bovine), Goat, Guinea Pig, Hamster, Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus), Sheep (Ovine)
525
253
213
81
61
56
43
39
34
28
26
22
22
17
15
15
15
13
10
10
10
9
7
5
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
345
209
18
1
1
Clonality
Polyclonal
Conjugate
This beta Actin antibody is un-conjugated
32
25
17
5
5
4
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
505
222
160
103
95
61
49
41
16
13
12
12
10
8
6
6
3
2
2
2
2
1
1
1
1
Supplier
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Immunogen Synthetic peptide: KQEYDESGPSC conjugated to KLH, corresponding to amino acids 359-368 of Human beta Actin.
Cross-Reactivity (Details) Expected to cross-react with a wide range of species including most vertebrates,some yeast due to sequence identity.
Purification Immunogen affinity purified
Alternative Name beta-Actin (ACTB Antibody Abstract)
Background Gene Symbol: ACTB
Molecular Weight Theoretical MW: 42 kDa
UniProt P60709
Application Notes Western Blot 1:1000-1:4000, ELISA 1:10000-1:40000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:500-1:2000, Immunohistochemistry-Paraffin 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.3 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (ABIN153390) Detection of actin using ABIN153390. Lane 1: HeLa cell lysate, Lane 2: rat brain, Lan...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (ABIN153390) Figure 1. Western blot using anti-beta Actin antibody shows detection of a predomina...
Immunofluorescence (IF) image for anti-beta Actin antibody (Actin, beta) (ABIN153390) Figure 2. Immunohistochemical staining using Affinity Purified anti-beta Actin antibo...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (ABIN153390) Western Blot: beta-Actin Antibody [ABIN153390] - analysis of Beta Actin on HeLa and L...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (ABIN153390) Western Blot: beta-Actin Antibody [ABIN153390] - Lane 1: molecular weight. Lane 2: hu...
Product cited in: Gu, Helenius, Väänänen, Bulanova, Saarela, Sokolenko, Martens, Imyanitov, Kuznetsov: "BRCA1-deficient breast cancer cell lines are resistant to MEK inhibitors and show distinct sensitivities to 6-thioguanine." in: Scientific reports, Vol. 6, pp. 28217, 2016 (PubMed). Further details: Western Blotting

Bigot, Colli, Machiela, Jessop, Myers, Carrouget, Wagner, Roberson, Eymerit, Henrion, Chanock: "Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41." in: Nature communications, Vol. 7, pp. 12098, 2016 (PubMed). Further details: Western Blotting

Gerrits, Paradé, Koonen-Reemst, Bakker, Timmer-Hellings, Sollewijn Gelpke, Gossen: "Reversible infertility in a liver receptor homologue-1 (LRH-1)-knockdown mouse model." in: Reproduction, fertility, and development, Vol. 26, Issue 2, pp. 293-306, 2014 (PubMed). (Sample species: Mouse (Murine)).

Chang, Patel, Gwede, Morgado, Tomasevich, Fong, Farach-Carson, Delk: "IL-1? induces p62/SQSTM1 and represses androgen receptor expression in prostate cancer cells." in: Journal of cellular biochemistry, Vol. 115, Issue 12, pp. 2188-97, 2014 (PubMed).

Gu, Bouwman, Greco, Saarela, Yadav, Jonkers, Kuznetsov: "Suppression of BRCA1 sensitizes cells to proteasome inhibitors." in: Cell death & disease, Vol. 5, pp. e1580, 2014 (PubMed).

Ozkucur, Perike, Sharma, Funk: "Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness." in: BMC cell biology, Vol. 12, pp. 4, 2011 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Denis, Palmer-Smith, Elisma, Busuttil, Wright, Bou Khalil, Prat, Seidah, Chrétien, Mayne, Figeys: "Quantitative proteomic analysis of PCSK9 gain of function in human hepatic HuH7 cells." in: Journal of proteome research, Vol. 10, Issue 4, pp. 2011-26, 2011 (PubMed).

Mitchell, Lau, Lambert, Zhou, Fong, Couture, Figeys, Baetz: "Regulation of septin dynamics by the Saccharomyces cerevisiae lysine acetyltransferase NuA4." in: PLoS ONE, Vol. 6, Issue 10, pp. e25336, 2011 (PubMed).

Young, Guttman, Vaid, Vogl: "Tubulobulbar complexes are intercellular podosome-like structures that internalize intact intercellular junctions during epithelial remodeling events in the rat testis." in: Biology of reproduction, Vol. 80, Issue 1, pp. 162-74, 2008 (PubMed). Further details: Immunocytochemistry,Immunoelectron Microscopy,Immunofluorescence

Lee, Bone, Strege, Shanafelt, Jelinek, Kay: "VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia." in: Blood, Vol. 104, Issue 3, pp. 788-94, 2004 (PubMed).