Actin antibody

Antigen: Actin

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Clonality: Polyclonal

Host: Rabbit

Reactivity: Chicken

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), ELISA

Catalog no.: ABIN153390

Additional Information

Alternative name: Actin (pan)

Gene ID: 60, 11461

Immunogen: Synthetic peptide: KQEYDESGPSC conjugated to KLH, corresponding to amino acids359-368 of Human beta Actin.

Cross-Reactivity: Chicken, Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis

Description: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have beenidentified. The alpha actins are found in muscle tissues and are a major constituent ofthe contractile apparatus. The beta and gamma actins coexist in most cell types ascomponents of the cyto-skeleton and as mediators of internal cell motility. Beta actinsare highly conserved proteins that are involved in cell motility, structure and integrity.Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells.Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in theform of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as anexcellent loading control. Alternate Names: anti-ActG antibody, anti-ActG1 antibody, anti-Actin antibody, anti-Gamma1 antibody,anti-DFNA20 antibody.

Specificity: This antibody recognizes beta and gamma actin in human samples. It is expected torecognise all the other known forms of human actin. This antibody detects a band atapproximately 42 kDa. Species Reactivity: Expected to cross-react with a wide range of species due to sequence homology. Localization: Cytoplasmic.

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Application Details

Application Notes: Suggested working dilutions: ELISA 1:10000 - 1:40000, immunofluorescence 1:500 - 1:2000, Western Blot 1:1000 - 1:4000. Positive Controls: HeLa whole cell lysate, HEK 293, HLC A549, mouse brain cells

Concentration: 1.02 mg/ml

Purification: Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Preservative: 0.01% Sodium Azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Images

Detection of actin using ABIN153390. Lane 1: HeLa cell lysate, Lane 2: rat brain, Lane 3: mouse brain, Lane 4: 3T3 cell lysate.

Figure 1. Western blot using anti-beta Actin antibody shows detection of a predominant band at ~42 kDa corresponding to beta Actin (arrowhead) in various whole cell lysates. Lysates are as follows: human embryonic kidney 293 (lane 2), human lung carcinoma A549 (lane 3) and mouse brain (lane 4) all using the 800 nm channel (green). ~ 35 ?g of each lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4? C followed by washes and reaction with a 1:10,000 dilution of IRDye?800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Molecular weight markers are shown in lane 1 using the 700 nm channel (red). IRDye?800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Figure 2. Immunohistochemical staining using Affinity Purified anti-beta Actin antibody at 1:200 shows detection of actin at the neuromuscular juntion of rana pipiens tissue fixed in 4% paraformaldehyde using a section 4.2 ?m thick. Personal Communication. S. Moeckel-Cole, Univ. MA, Amherst, MA.

Publications

Lee, Bone, Strege et al.: "VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia." in: Blood, Vol. 104, Issue 3, pp. 788-94, 2004 (PubMed).