Tubulin antibody (TUB)

Details for Product anti-TUB Antibody No. ABIN153391, Supplier: Log in to see
Antigen
Reactivity
Bird (Avian), Fruit Fly (Drosophila melanogaster), Human, Mammalian, Monkey, Mouse (Murine), Primate, Rat (Rattus), Yeast
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39
34
32
22
19
18
18
14
10
4
3
2
2
1
1
1
1
1
1
1
1
Host
Rat
33
33
23
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Tubulin antibody is un-conjugated
3
3
3
3
3
3
3
3
3
3
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3
3
3
3
2
2
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
82
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51
44
43
38
36
15
9
3
1
1
1
Options
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Immunogen Full length native protein (purified) (S. cerevisiae).
Clone YL1-2
Isotype IgG2a
Purification Protein G purified
Alternative Name Tubulin
Background Gene Symbol: TUBA1A
Gene ID 7846
Research Area Neurology, Cell/Tissue Markers, Cytoskeleton
Application Notes Western Blot 1:5000-1:10000, Flow Cytometry, ELISA 1:100-1:1000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:1000-1:10000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry-Frozen 1:200, RadioimmunoassayThis Tubulin Antibody (YL1/2) is useful for Western blot, Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Radioimmunoassay and ELISA. NB600-506 is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in Immunocytochemistry.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol Specific for NB600-506 Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Tubulin antibody (TUB) (ABIN153391) Western blot using ABIN153391. Lane 1 and 2: HeLa whole cell lysate, Lane 3 and 4: 3T...
Western Blotting (WB) image for anti-Tubulin antibody (TUB) (ABIN153391) Western Blot: Tubulin Antibody (YL1/2) [ABIN153391] - Western blot analysis of Tubuli...
Flow Cytometry (FACS) image for anti-Tubulin antibody (TUB) (ABIN153391) Flow Cytometry: Tubulin Antibody (YL1/2) [ABIN153391] - Analysis of Alexa Fluor (R) 6...
Western Blotting (WB) image for anti-Tubulin antibody (TUB) (ABIN153391) Western Blot: Tubulin Antibody (YL1/2) [ABIN153391] - WB analysis of whole cell lysat...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tubulin antibody (TUB) (ABIN153391) Immunohistochemistry-Paraffin: Tubulin Antibody (YL1/2) [ABIN153391] - Tubulin antibo...
Immunofluorescence (IF) image for anti-Tubulin antibody (TUB) (ABIN153391) Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [ABIN153391] - IF Co...
Immunofluorescence (IF) image for anti-Tubulin antibody (TUB) (ABIN153391) Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [ABIN153391] - B-Tub...
Immunofluorescence (IF) image for anti-Tubulin antibody (TUB) (ABIN153391) Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [ABIN153391] - Tubul...
Immunofluorescence (IF) image for anti-Tubulin antibody (TUB) (ABIN153391) Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [ABIN153391] - Tubul...
Immunofluorescence (IF) image for anti-Tubulin antibody (TUB) (ABIN153391) Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [ABIN153391] - Tubul...
Product cited in: Comaills, Kabeche, Morris, Buisson, Yu, Madden, LiCausi, Boukhali, Tajima, Pan, Aceto, Sil, Zheng, Sundaresan, Yae, Jordan, Miyamoto, Ting, Ramaswamy, Haas, Zou, Haber, Maheswaran: "Genomic Instability Is Induced by Persistent Proliferation of Cells Undergoing Epithelial-to-Mesenchymal Transition." in: Cell reports, Vol. 17, Issue 10, pp. 2632-2647, 2016 (PubMed). Method employed by authors: Immunofluorescence (IF)

Hu, Lu, Orr, Godek, Mustachio, Kawakami, Sekula, Compton, Freemantle, Dmitrovsky: "Specific CP110 Phosphorylation Sites Mediate Anaphase Catastrophe after CDK2 Inhibition: Evidence for Cooperation with USP33 Knockdown." in: Molecular cancer therapeutics, Vol. 14, Issue 11, pp. 2576-85, 2015 (PubMed).

Sung, Giannakakou: "BRCA1 regulates microtubule dynamics and taxane-induced apoptotic cell signaling." in: Oncogene, Vol. 33, Issue 11, pp. 1418-28, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Ganem, Cornils, Chiu, ORourke, Arnaud, Yimlamai, Théry, Camargo, Pellman: "Cytokinesis failure triggers hippo tumor suppressor pathway activation." in: Cell, Vol. 158, Issue 4, pp. 833-48, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Girón-Martínez, Pérez-Rial, Terrón-Expósito, Díaz-Gil, González-Mangado, Peces-Barba: "Proliferative activity of liver growth factor is associated with an improvement of cigarette smoke-induced emphysema in mice." in: PLoS ONE, Vol. 9, Issue 11, pp. e112995, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Batista-Nascimento, Toledano, Thiele, Rodrigues-Pousada: "Yeast protective response to arsenate involves the repression of the high affinity iron uptake system." in: Biochimica et biophysica acta, Vol. 1833, Issue 5, pp. 997-1005, 2013 (PubMed). Further details: Western Blotting

Whitfield, Chisholm, Hawley, Orr-Weaver: "A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony." in: PLoS biology, Vol. 11, Issue 9, pp. e1001648, 2013 (PubMed). (Sample species: Fruit Fly (Drosophila melanogaster)). Further details: Western Blotting

Bhattacharya, Gonzalez, Debiase, Trejo, Goldman, Flitney, Jones: "Recruitment of vimentin to the cell surface by beta3 integrin and plectin mediates adhesion strength." in: Journal of cell science, Vol. 122, Issue Pt 9, pp. 1390-400, 2009 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Ganem, Godinho, Pellman: "A mechanism linking extra centrosomes to chromosomal instability." in: Nature, Vol. 460, Issue 7252, pp. 278-82, 2009 (PubMed). (Sample species: Rat (Rattus)).

OConnell, Loncarek, Kaláb, Khodjakov: "Relative contributions of chromatin and kinetochores to mitotic spindle assembly." in: The Journal of cell biology, Vol. 187, Issue 1, pp. 43-51, 2009 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Yang, Kenny, Brito, Rieder: "Cells satisfy the mitotic checkpoint in Taxol, and do so faster in concentrations that stabilize syntelic attachments." in: The Journal of cell biology, Vol. 186, Issue 5, pp. 675-84, 2009 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Liang, Mukherjee, Shen, Maxfield, Gottesman: "Endocytic recycling compartments altered in cisplatin-resistant cancer cells." in: Cancer research, Vol. 66, Issue 4, pp. 2346-53, 2006 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Iida, Hirota, Morisaki, Marumoto, Hara, Kuninaka, Honda, Kosai, Kawasuji, Pallas, Saya: "Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and G1 tetraploidy checkpoint function." in: Oncogene, Vol. 23, Issue 31, pp. 5266-74, 2004 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Allen, Brinkhuis, van Deemter, Wijnholds, Schinkel: "Extensive contribution of the multidrug transporters P-glycoprotein and Mrp1 to basal drug resistance." in: Cancer research, Vol. 60, Issue 20, pp. 5761-6, 2000 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Breitling, Dübel, Seehaus, Klewinghaus, Little: "A surface expression vector for antibody screening." in: Gene, Vol. 104, Issue 2, pp. 147-53, 1991 (PubMed).

Background publications Clayton, Lloyd: "The relationship between the division plane and spindle geometry in Allium cells treated with CIPC and griseofulvin: an anti-tubulin study." in: European journal of cell biology, Vol. 34, Issue 2, pp. 248-53, 1984 (PubMed).

Kilmartin, Wright, Milstein: "Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line." in: The Journal of cell biology, Vol. 93, Issue 3, pp. 576-82, 1982 (PubMed).