alpha Tubulin / TUBA1B (Tyr-Tubulin) antibody

Details for Product No. ABIN153391
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Bird (Avian), Mammalian, Yeast
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(1), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN153391
Quantity 0.1 mg
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Immunogen Full length native protein (purified) (S. cerevisiae).
Clone YL1-2
Isotype IgG2a
Specificity This antibody detects the tyrosinated form of the alpha-tubulin subunit. It has equalaffinity for fixed microtubules (formaldehyde or glutaraldehyde) and native microtubules.The antibody recognizes an extremely short amino acid sequence Glu-Glu-Phe(OH)which can be inserted into the C-terminal domain of fusion proteins. Species Reactivity: S. cerevisiae, S. pombe, Slime moulds, Allium, birds and all Mammals. Expected toreact with most eukaryotes due to sequence homology. Other species have not beentested.
Cross-Reactivity Bird (Avian), Mammalian, Yeast
Purification Purified
Background This antibody can be used as a loading control on Western blots (Allen et al.) and is notdetected by anti-mouse Ig secondaries.It has been used in epitope tagging proceduresto detect proteins tagged with a C-terminal Gly-Gly-Phe(OH) epitope. Under somecircumstances this antibody may cross-react with other protein including E. coli rec Aand oxidized actin. Alternate Names: anti-Alpha tubulin ubiquitous antibody, anti-H2 ALPHA antibody, anti-TUBA1 antibody,anti-Tubulin alpha 1 chain antibody, anti-Tubulin alpha antibody, anti-Tubulin alpha ubiquitous chain antibody, anti-Tubulin K alpha 1 antibody.
Gene ID 7846
Application Notes Suggested working dilutions: ELISA 1:100-1:1000,Radioimmunoassay
Restrictions For Research Use only
Concentration 1.0 mg/ml
Buffer PBS, [pH7.4]. Preservative: 0.09% sodium azide.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-alpha Tubulin / TUBA1B (Tyr-Tubulin) antibody Western blot using ABIN153391. Lane 1 and 2: HeLa whole cell lysate, Lane 3 and 4: 3T3 (mouse) cell lysate.
General Breitling, Duebel, Seehaus et al.: "A surface expression vector for antibody screening." in: Gene, Vol. 104, Issue 2, pp. 147-53, 1991 (PubMed).

Clayton, Lloyd: "The relationship between the division plane and spindle geometry in Allium cells treated with CIPC and griseofulvin: an anti-tubulin study." in: European journal of cell biology, Vol. 34, Issue 2, pp. 248-53, 1984 (PubMed).

Kilmartin, Wright, Milstein: "Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line." in: The Journal of cell biology, Vol. 93, Issue 3, pp. 576-82, 1982 (PubMed).

Allen, Brinkhuis, van Deemter et al.: "Extensive contribution of the multidrug transporters P-glycoprotein and Mrp1 to basal drug resistance." in: Cancer research, Vol. 60, Issue 20, pp. 5761-6, 2000 (PubMed).

Iida, Hirota, Morisaki et al.: "Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and G1 tetraploidy checkpoint function." in: Oncogene, Vol. 23, Issue 31, pp. 5266-74, 2004 (PubMed).

Hosts (1)
Reactivities (1)
Applications (1), (1), (1), (1)
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