alpha Tubulin / TUBA1B (Tyr-Tubulin) antibody

Details for Product No. ABIN153391
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Antigen
Reactivity
Bird (Avian), Mammalian, Yeast
(1)
Host
Rat
(1)
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(1), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN153391
Quantity 0.1 mg
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Immunogen Full length native protein (purified) (S. cerevisiae).
Clone YL1-2
Isotype IgG2a
Specificity This antibody detects the tyrosinated form of the alpha-tubulin subunit. It has equalaffinity for fixed microtubules (formaldehyde or glutaraldehyde) and native microtubules.The antibody recognizes an extremely short amino acid sequence Glu-Glu-Phe(OH)which can be inserted into the C-terminal domain of fusion proteins. Species Reactivity: S. cerevisiae, S. pombe, Slime moulds, Allium, birds and all Mammals. Expected toreact with most eukaryotes due to sequence homology. Other species have not beentested.
Cross-Reactivity Bird (Avian), Mammalian, Yeast
Purification Purified
Background This antibody can be used as a loading control on Western blots (Allen et al.) and is notdetected by anti-mouse Ig secondaries.It has been used in epitope tagging proceduresto detect proteins tagged with a C-terminal Gly-Gly-Phe(OH) epitope. Under somecircumstances this antibody may cross-react with other protein including E. coli rec Aand oxidized actin. Alternate Names: anti-Alpha tubulin ubiquitous antibody, anti-H2 ALPHA antibody, anti-TUBA1 antibody,anti-Tubulin alpha 1 chain antibody, anti-Tubulin alpha antibody, anti-Tubulin alpha ubiquitous chain antibody, anti-Tubulin K alpha 1 antibody.
Gene ID 7846
Application Notes Suggested working dilutions: ELISA 1:100-1:1000,Radioimmunoassay
Restrictions For Research Use only
Concentration 1.0 mg/ml
Buffer PBS, [pH7.4]. Preservative: 0.09% sodium azide.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-alpha Tubulin / TUBA1B (Tyr-Tubulin) antibody Western blot using ABIN153391. Lane 1 and 2: HeLa whole cell lysate, Lane 3 and 4: 3T3 (mouse) cell lysate.
General Breitling, Duebel, Seehaus et al.: "A surface expression vector for antibody screening." in: Gene, Vol. 104, Issue 2, pp. 147-53, 1991 (PubMed).

Clayton, Lloyd: "The relationship between the division plane and spindle geometry in Allium cells treated with CIPC and griseofulvin: an anti-tubulin study." in: European journal of cell biology, Vol. 34, Issue 2, pp. 248-53, 1984 (PubMed).

Kilmartin, Wright, Milstein: "Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line." in: The Journal of cell biology, Vol. 93, Issue 3, pp. 576-82, 1982 (PubMed).

Allen, Brinkhuis, van Deemter et al.: "Extensive contribution of the multidrug transporters P-glycoprotein and Mrp1 to basal drug resistance." in: Cancer research, Vol. 60, Issue 20, pp. 5761-6, 2000 (PubMed).

Iida, Hirota, Morisaki et al.: "Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and G1 tetraploidy checkpoint function." in: Oncogene, Vol. 23, Issue 31, pp. 5266-74, 2004 (PubMed).

Hosts (1)
Reactivities (1)
Applications (1), (1), (1), (1)
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