Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Add to Basket
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN153391

alpha Tubulin / TUBA1B (Tyr-Tubulin) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
»Alternatives Bird (Avian), Mammalian, Yeast
Host
Clonality (Clone) Monoclonal ()
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN153391
Quantity 0.1 mg
Price
Contact our Customer Service for availability and price in your country.
Shipping to
Immunogen Full length native protein (purified) (S. cerevisiae).
Clone YL1-2
Isotype IgG2a
Specificity This antibody detects the tyrosinated form of the alpha-tubulin subunit. It has equalaffinity for fixed microtubules (formaldehyde or glutaraldehyde) and native microtubules.The antibody recognizes an extremely short amino acid sequence Glu-Glu-Phe(OH)which can be inserted into the C-terminal domain of fusion proteins. Species Reactivity: S. cerevisiae, S. pombe, Slime moulds, Allium, birds and all Mammals. Expected toreact with most eukaryotes due to sequence homology. Other species have not beentested.
Cross-Reactivity Bird (Avian), Mammalian, Yeast
Purification Purified
Background This antibody can be used as a loading control on Western blots (Allen et al.) and is notdetected by anti-mouse Ig secondaries.It has been used in epitope tagging proceduresto detect proteins tagged with a C-terminal Gly-Gly-Phe(OH) epitope. Under somecircumstances this antibody may cross-react with other protein including E. coli rec Aand oxidized actin. Alternate Names: anti-Alpha tubulin ubiquitous antibody, anti-H2 ALPHA antibody, anti-TUBA1 antibody,anti-Tubulin alpha 1 chain antibody, anti-Tubulin alpha antibody, anti-Tubulin alpha ubiquitous chain antibody, anti-Tubulin K alpha 1 antibody.
Gene ID 7846
Application Notes Suggested working dilutions: ELISA 1:100-1:1000,Radioimmunoassay
Restrictions For Research Use only
Concentration 1.0 mg/ml
Buffer PBS, [pH7.4]. Preservative: 0.09% sodium azide.
Preservative Sodium azide
Storage 4 °C
Breitling, Duebel, Seehaus et al.: "A surface expression vector for antibody screening." in: Gene, Vol. 104, Issue 2, pp. 147-53, 1991 (PubMed).

Clayton, Lloyd: "The relationship between the division plane and spindle geometry in Allium cells treated with CIPC and griseofulvin: an anti-tubulin study." in: European journal of cell biology, Vol. 34, Issue 2, pp. 248-53, 1984 (PubMed).

Kilmartin, Wright, Milstein: "Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line." in: The Journal of cell biology, Vol. 93, Issue 3, pp. 576-82, 1982 (PubMed).

Allen, Brinkhuis, van Deemter et al.: "Extensive contribution of the multidrug transporters P-glycoprotein and Mrp1 to basal drug resistance." in: Cancer research, Vol. 60, Issue 20, pp. 5761-6, 2000 (PubMed).

Iida, Hirota, Morisaki et al.: "Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and G1 tetraploidy checkpoint function." in: Oncogene, Vol. 23, Issue 31, pp. 5266-74, 2004 (PubMed).

Alternatives for antigen "alpha Tubulin / TUBA1B (Tyr-Tubulin)", type "Antibodies"
Hosts (3)
Reactivities (3)
Applications (3), (2), (1), (1), (1)
Beacon