Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody

Details for Product No. ABIN153416
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Antigen
Synonyms ASMA, SMactin, a-sma, aat6, actsa, mymy5, sm-actin, ACTA2, actc1, ACT-4, AAT6, ACTSA, MYMY5, 0610041G09Rik, Actvs, SMalphaA, a-SMA, alphaSMA, wu:fb63d03, asma
Epitope
N-Term
(74), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(139), (51), (39), (15), (11), (6), (6), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(82), (64), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(99), (66), (55), (47), (32), (20), (19), (19), (6), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.5 mL
Shipping to United States (Change)
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Catalog No. ABIN153416
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Immunogen Synthetic peptide corresponding to N-terminus of human alpha smooth muscle isoform of actin.
Specificity This stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells.
Cross-Reactivity (Details) Predicted cross-reactivity based on sequence identity: Bovine, Chicken, Frog, Pig, Rabbit.
Purification affinity purified
Alternative Name Aortic smooth muscle Actin / ACTA2
Background Actins are highly conserved proteins expressed in all eukaryotic cells. Actin filamentsform part of the cytoskeleton and play essential roles in regulating cell shape andmovement. Six distinct actin isotypes have been identified in mammalian cells. Each isencoded by a separated gene and is expressed in a developmentally regulated andtissue-specific manner, alpha and beta cytoplasmic actins are expressed in a widevariety of cells, whereas, expression of alpha skeletal, alpha cardiac, alpha vascular,and gamma enteric actins are more restricted to specialized muscle cell type. Smoothmuscle alpha actin is of further interest because it is one of a few genes whoseexpression is relatively restricted to vascular smooth muscle cells. Further more,expression of smooth muscle alpha actin is regulated by hormones, cell proliferation ,and altered by pathological conditions including oncogenic transformation andatherosclerosis. Alternate Names: anti-Smooth Muscle Actin antibody.
Gene Symbol: ACTA2
Gene ID 59, 25365
Research Area Extracellular Matrix, Cell/Tissue Markers
Application Notes Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:400, Western Blot 1:100-1:2000
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS/1 % BSA buffer pH 7.6, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody Formalin-fixed, paraffin-embedded Human Leiomyoma stained with ABIN153416
General Jeyasuria, Wetzel, Bradley et al.: "Progesterone-regulated caspase 3 action in the mouse may play a role in uterine quiescence during pregnancy through fragmentation of uterine myocyte contractile proteins." in: Biology of reproduction, Vol. 80, Issue 5, pp. 928-34, 2009 (PubMed).

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