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Actin, beta (ACTB) antibody

Antigen

Actin, beta (ACTB)
Synonyms
PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A19 ... show more
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
3 references available
Catalog no. ABIN153417
Quantity 0.1 mg  (1 mg/ml)  (Variants)
Price Product not available in this region.
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anti-Actin, beta (ACTB) antibody

Additional Information
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Alternative name Actin beta / ACTB
Gene ID 60, 11465
UniProt P60709
Immunogen The epitope recognized by this antibody maps to the N-terminus of human Beta-actinusing the numbering given in Swiss-Prot entry P60709 (GeneID 60).
Cross-Reactivity Human, Mouse (Murine)
Description In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have beenidentified. The alpha actins are found in muscle tissues and are a major constituent ofthe contractile apparatus. The beta and gamma actins coexist in most cell types ascomponents of the cyto-skeleton and as mediators of internal cell motility. Beta actinsare highly conserved proteins that are involved in cell motility, structure and integrity.Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells.Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in theform of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as anexcellent loading control. Alternate Names: anti-ActG antibody, anti-ActG1 antibody, anti-Actin antibody, anti-Gamma1 antibody,anti-DFNA20 antibody.
Specificity Detects a single clean band at 40kD representing actin. Species Reactivity: Human and Mouse.
Synonyms PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Application Details
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Application Notes Suggested working dilutions: immunoprecipitation 2-5 ug/mg,Western Blot 1:5,000-1:15,000
Concentration 1 mg/ml
Purification Affinity Purified
Buffer Tris-citrate/phosphate buffer, pH 7 to 8. Preservative: 0.09% Sodium Azide.
Storage Store at 4C. Do not freeze.
Restrictions For Research Use only

Publications
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Watters, Kraja, Meucci et al.: "Genome-wide discovery of loci influencing chemotherapy cytotoxicity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 32, pp. 11809-14, 2004 (PubMed).

Sanue, Sinnett, Delvin et al.: "Localization and role of NPC1L1 in cholesterol absorption in human intestine." in: Journal of lipid research, Vol. 47, Issue 10, pp. 2112-20, 2006 (PubMed).

Boutz, Chawla, Stoilov et al.: "MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development." in: Genes & development, Vol. 21, Issue 1, pp. 71-84, 2007 (PubMed).

Alternatives
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Alternatives for antigen "Actin, beta (ACTB)", type "Antibodies"
Hosts Rabbit (120), Mouse (81), Chicken (12), Guinea Pig (3)
Reactivities Human (197), Rat (Rattus) (102), Mouse (Murine) (98), Chicken (38), Cow (Bovine) (35), Pig (Porcine) (35), Sheep (Ovine) (32), Guinea Pig (28), Dog (Canine) (27), Cat (Feline) (26), Rabbit (23), Monkey (10), Hamster (7), Carp (3), Goat (3), All Species (2), Fruit Fly (Drosophila melanogaster) (2), Xenopus laevis (2), Zebrafish (Danio rerio) (2), Arabidopsis (1), Caenorhabditis elegans (C. elegans) (1), Leech (1), Nicotiana tabacum (1), Primate (1), Rice (Oryza sativa) (1)
Applications Western Blotting (WB) (189), ELISA (100), Immunofluorescence (IF) (57), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Immunohistochemistry (IHC) (43), Immunocytochemistry (ICC) (27), Immunoprecipitation (IP) (15), Flow Cytometry (FACS) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Dot Blot (Dot) (4), Fluorescence Microscopy (FM) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Dot Blot (DB) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
Conjugates Biotin (6), HRP (6), FITC (4), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes N-Term (48), C-Term (10), AA 359-368 (7), Internal Region (3), AA 2-16 (2), Cytoplasmic Domain (2), Cytoplasmic,N-Term (2), AA 1-14 (1), AA 1-375 (1), AA 1-376 (1)