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beta Actin antibody (Actin, beta) (N-Term)

Details for Product anti-ACTB Antibody No. ABIN153418, Supplier: Log in to see
Antigen
  • beta-actin
  • ps1tp5bp1
  • LOC563571
  • ACTB
  • A26C1A
  • A26C1B
  • act-2
  • actb
  • B-actin
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • BRWS1
  • PS1TP5BP1
  • ACT-5
  • Actx
  • E430023M04Rik
  • ACTA1
  • Beta-actin
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • POTE2alpha
  • POTEACTIN
  • actin, beta
  • similar to beta-cytoplasmic actin2
  • cytoplasmic actin OlCA1
  • actin beta
  • beta-actin
  • Actin 5C
  • actin, beta 1
  • actin, gamma 1
  • beta actin
  • POTE ankyrin domain family, member F
  • actb
  • LOC563571
  • ACTB
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • Act5C
  • Actb
  • actb1
  • ACTG1
  • LOC396797
  • LOC100350495
  • LOC100136352
  • POTEF
Epitope
N-Term
165
39
16
15
10
9
7
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
553
279
238
80
61
56
43
40
39
34
32
29
27
22
18
15
15
13
11
10
10
10
7
6
5
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
355
230
18
1
1
Clonality
Polyclonal
Conjugate
This beta Actin antibody is un-conjugated
33
26
18
6
6
5
5
4
4
4
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
525
239
165
122
108
80
57
40
17
12
12
11
9
8
3
2
2
2
2
2
2
1
1
1
1
Options
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Immunogen A region corresponding to the N-terminus of human Beta Actin [UniProt# P60709]
Purification Immunogen affinity purified
Alternative Name beta-Actin (ACTB Antibody Abstract)
Background Gene Symbol: ACTB
Molecular Weight Theoretical MW: 40 kDa
Gene ID 60
UniProt P60709
Application Notes Western Blot 1:5000-1:15000, Simple Western 1:2000, ImmunohistochemistryThis antibody is useful for Western Blot. A 40 kDa band is detected in HeLa whole cell lysate and mouse NIH3T3 cells. This antibody is also useful for immunocytochemistry/immunofluorescence. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Not for use in: ICC/IF
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Beta Actin Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 20 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-actin primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interferewith antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153418) anti-Actin, beta (ACTB) (N-Term) antibody
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153418) Western Blot: beta-Actin Antibody [ABIN153417] - Detection of actin in 3T3 (20ug) lys...
Simple Western (SimWes) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153418) Simple Western: beta-Actin Antibody [ABIN153417] - Simple Western lane view shows a s...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153418) Western Blot: beta-Actin Antibody [ABIN153417] - Western blot analysis of Actin (ABIN...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153418) Western Blot: beta-Actin Antibody [ABIN153417] - Western blot analysis of Actin (ABIN...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153418) Western Blot: beta-Actin Antibody [ABIN153417] - Detection of Beta Actin. Lane 1: 5ug...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN153418) Western Blot: beta-Actin Antibody [ABIN153417] - Analysis using the HRP conjugate of ...
Product cited in: Wilky, Kim, McCarty, Montgomery, Kammers, DeVine, Cole, Raman, Loeb: "RNA helicase DDX3: a novel therapeutic target in Ewing sarcoma." in: Oncogene, Vol. 35, Issue 20, pp. 2574-83, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Samura, Morikage, Suehiro, Tanaka, Nakamura, Nishimoto, Ueno, Hosoyama, Hamano: "Combinatorial Treatment with Apelin-13 Enhances the Therapeutic Efficacy of a Preconditioned Cell-Based Therapy for Peripheral Ischemia." in: Scientific reports, Vol. 6, pp. 19379, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Li, Li, Chen, Jin, Su, Liu, Duan, Li, Qi, Shi, Ni, Yang, Gui, Mao, Chen, Lai: "miR‑30a‑5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4085-94, 2016 (PubMed).

Li, Chen, Li, Jin, Liu, Su, Qi, Shi, Jiang, Ni, Yang, Gui, Mao, Chen, Lai: "Oncogenic cAMP responsive element binding protein 1 is overexpressed upon loss of tumor suppressive miR-10b-5p and miR-363-3p in renal cancer." in: Oncology reports, Vol. 35, Issue 4, pp. 1967-78, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Su, Chen, Zhang, Li, Yu, Shi, Jiang, Ni, Yang, Gui, Ye, Lai: "MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 1535-43, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Fine, Renner, Forsberg, Cameron, Galick, Le, Conway, Stroebel, Frey, Hanson: "Intranasal deferoxamine engages multiple pathways to decrease memory loss in the APP/PS1 model of amyloid accumulation." in: Neuroscience letters, Vol. 584, pp. 362-7, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Yang, Hsu, Lin, Guo, Yu, Chang: "Krüppel-like factor 10 upregulates the expression of cyclooxygenase 1 and further modulates angiogenesis in endothelial cell and platelet aggregation in gene-deficient mice." in: The international journal of biochemistry & cell biology, Vol. 45, Issue 2, pp. 419-28, 2013 (PubMed). Further details: Western Blotting

Cheng, Lu, Tseng, Sun, Lin, Kuo, Pan, Ko: "Antitumor Effect of Periplocin in TRAIL-Resistant Human Hepatocellular Carcinoma Cells through Downregulation of IAPs." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 958025, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Chang, Yang, Lin, Pearce, Ryan, Chen: "IbACP, a sixteen-amino-acid peptide isolated from Ipomoea batatas leaves, induces carcinoma cell apoptosis." in: Peptides, Vol. 47, pp. 148-56, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Matthaei, Budt, Wolff: "Highly pathogenic H5N1 influenza A virus strains provoke heterogeneous IFN-α/β responses that distinctively affect viral propagation in human cells." in: PLoS ONE, Vol. 8, Issue 2, pp. e56659, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Meerson, Traurig, Ossowski, Fleming, Mullins, Baier: "Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-α." in: Diabetologia, Vol. 56, Issue 9, pp. 1971-9, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Pucer, Brglez, Payré, Pungerčar, Lambeau, Petan: "Group X secreted phospholipase A(2) induces lipid droplet formation and prolongs breast cancer cell survival." in: Molecular cancer, Vol. 12, Issue 1, pp. 111, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Cmarik, Troxler, Hanson, Zhang, Ruscetti: "The human lung adenocarcinoma cell line EKVX produces an infectious xenotropic murine leukemia virus." in: Viruses, Vol. 3, Issue 12, pp. 2442-61, 2012 (PubMed).

Fine, Baillargeon, Renner, Hoerster, Tokarev, Colton, Pelleg, Andrews, Sparley, Krogh, Frey, Hanson: "Intranasal deferoxamine improves performance in radial arm water maze, stabilizes HIF-1α, and phosphorylates GSK3β in P301L tau transgenic mice." in: Experimental brain research, Vol. 219, Issue 3, pp. 381-90, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Chang, Chu, Peng, Mao, Shan, Hsu, Lin, Tsai, Yu, Chang: "Krüppel-like factor 10 expression as a prognostic indicator for pancreatic adenocarcinoma." in: The American journal of pathology, Vol. 181, Issue 2, pp. 423-30, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Hsu, Sui, Wu, Wang, Yang, Chen, Yu, Chang: "Klf10 induces cell apoptosis through modulation of BI-1 expression and Ca2+ homeostasis in estrogen-responding adenocarcinoma cells." in: The international journal of biochemistry & cell biology, Vol. 43, Issue 4, pp. 666-73, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Mounce, Tsan, Droit, Kohler, Reitsma, Cirillo, Tarakanova: "Gammaherpesvirus gene expression and DNA synthesis are facilitated by viral protein kinase and histone variant H2AX." in: Virology, Vol. 420, Issue 2, pp. 73-81, 2011 (PubMed).

Zois, Giatromanolaki, Kainulainen, Botaitis, Torvinen, Simopoulos, Kortsaris, Sivridis, Koukourakis: "Lung autophagic response following exposure of mice to whole body irradiation, with and without amifostine." in: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 552-8, 2011 (PubMed). (Sample species: Mouse (Murine)).

Zois, Giatromanolaki, Sivridis, Papaiakovou, Kainulainen, Koukourakis: ""Autophagic flux" in normal mouse tissues: focus on endogenous LC3A processing." in: Autophagy, Vol. 7, Issue 11, pp. 1371-8, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Deng, Liu, Pritchard, Eisen, Zacksenhaus: "Preferential killing of breast tumor initiating cells by N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine/tesmilifene." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 1, pp. 119-30, 2009 (PubMed).