Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody

Details for Product No. ABIN153421
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Antigen
Synonyms Cd169, Siglec-1, Sn, CD169, SIGLEC-1, SN, dJ1009E24.1, SA, SIGLEC1, p210, pSn
Reactivity
Human
(35), (26), (8), (6)
Host
Mouse
(27), (22), (19), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(14), (3), (3)
Application
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro), Radioimmunoassay (RIA)
(47), (34), (17), (14), (13), (12), (10), (6), (3), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN153421
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Immunogen Full-length purified human Sialoadhesin (d1-4)Fc protein.
Clone HSn 7D2
Isotype IgG1
Cross-Reactivity (Details) Not yet tested in other species.
Purification Protein G purified
Alternative Name CD169 / SIGLEC1
Background The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily)that bind sialic acid and mediate cell-cell interactions. Family members includesialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesionmolecule that is expressed on activated macrophages in chronic inflammation and intumours. Alternate Names: anti-CD169 antibody, anti-Sialic Acid-binding Immunoglobulin-like Lectin 1 antibody,anti-Siglec 1 antibody.
Gene Symbol: SIGLEC1
Gene ID 6614
UniProt Q9BZZ2
Application Notes This Sialoadhesin Antibody (HSn 7D2) is useful for Immunohistochemistry (paraffin-embedded, acetone-fixed, and frozen sections), Flow Cytometry, Radioimmunoassay and ELISA. Western Blot was reported in scientific literature. Use in in vitro assay reported in scientific literature ( PMID 18414664)
Recommended dilutions: ELISA 1:100-1:2000, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Frozen 1:10-1:2000, Immunohistochemistry-Paraffin 1:10-1:2000, In vitro assay, Radioimmunoassay, Western Blot
Protocol IHC Protocol for NB600-534: Sialoadhesin Antibody (HSn 7D2)Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Hartnell, Steel, Turley et al.: "Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations." in: Blood, Vol. 97, Issue 1, pp. 288-96, 2001 (PubMed).

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