SIGLEC1 antibody (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin)

Details for Product anti-SIGLEC1 Antibody No. ABIN153421, Supplier: Log in to see
Antigen
  • Cd169
  • Siglec-1
  • Sn
  • CD169
  • SIGLEC-1
  • SN
  • dJ1009E24.1
  • SA
  • SIGLEC1
  • p210
  • pSn
  • sialic acid binding Ig-like lectin 1, sialoadhesin
  • sialoadhesin
  • SIGLEC1
  • LOC100345836
  • Siglec1
  • SIGLEC-1
Reactivity
Human, Pig (Porcine), Virus
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91
40
29
18
3
1
Host
Mouse
115
69
41
7
Clonality (Clone)
Monoclonal ()
Conjugate
This SIGLEC1 antibody is un-conjugated
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19
12
9
7
6
6
5
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
172
84
84
44
44
40
33
27
13
13
8
5
2
2
2
1
Options
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Immunogen Full-length purified human Sialoadhesin (d1-4)Fc protein. [Swiss-Prot# Q9BZZ2]
Clone HSn 7D2
Isotype IgG1
Purification Protein G purified
Alternative Name Siglec-1/CD169 (SIGLEC1 Antibody Abstract)
Target Type Virus
Background Gene Symbol: SIGLEC1
Gene ID 6614
UniProt Q9BZZ2
Application Notes Western Blot, Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, In vitro assay, RadioimmunoassayThis Sialoadhesin Antibody (HSn 7D2) is useful for Immunohistochemistry (paraffin-embedded, acetone-fixed, and frozen sections), Flow Cytometry, Radioimmunoassay and ELISA. Western Blot was reported in scientific literature. Use in in vitro assay reported in scientific literature ( PMID 18414664)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol IHC Protocol for NB600-534: Sialoadhesin Antibody (HSn 7D2)Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SIGLEC1 antibody (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin) (ABIN153421) Immunohistochemistry-Paraffin: Sialoadhesin Antibody (HSn 7D2) [ABIN153421] - IHC sta...
Product cited in: Groenendyk, Lee, Jung, Dyck, Lopaschuk, Agellon, Michalak: "Inhibition of the Unfolded Protein Response Mechanism Prevents Cardiac Fibrosis." in: PLoS ONE, Vol. 11, Issue 7, pp. e0159682, 2016 (PubMed). (Sample species: Pig (Porcine)). Further details: Immunohistochemistry

Yayi, Yeda, Huaxin, Yang, Qian, Zhongyuan: "Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats." in: Acta cirurgica brasileira, Vol. 31, Issue 7, pp. 448-55, 2016 (PubMed). (Sample species: Virus). Further details: Western Blotting

Novakovic, Harding, Ladinig, Al-Dissi, MacPhee, Detmer: "Relationships of CD163 and CD169 positive cell numbers in the endometrium and fetal placenta with type 2 PRRSV RNA concentration in fetal thymus." in: Veterinary research, Vol. 47, Issue 1, pp. 76, 2016 (PubMed). (Sample species: Pig (Porcine)). Further details: Immunohistochemistry

Akiyama, Ramirez, Gudheti, Gummuluru: "CD169-mediated trafficking of HIV to plasma membrane invaginations in dendritic cells attenuates efficacy of anti-gp120 broadly neutralizing antibodies." in: PLoS pathogens, Vol. 11, Issue 3, pp. e1004751, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting,Flow Cytometry

Kusumi, Koga, Kanda, Ushiki: "Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen." in: Biomedical research (Tokyo, Japan), Vol. 36, Issue 3, pp. 195-203, 2015 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Puryear, Akiyama, Geer, Ramirez, Yu, Reinhard, Gummuluru: "Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169." in: PLoS pathogens, Vol. 9, Issue 4, pp. e1003291, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Pack, Trumpfheller, Thomas, Park, Granelli-Piperno, Münz, Steinman: "DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp." in: Immunology, Vol. 123, Issue 3, pp. 438-46, 2008 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Rempel, Calosing, Sun, Pulliam: "Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity." in: PLoS ONE, Vol. 3, Issue 4, pp. e1967, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting,In vitro Assay,Flow Cytometry

Hartnell, Steel, Turley, Jones, Jackson, Crocker: "Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations." in: Blood, Vol. 97, Issue 1, pp. 288-96, 2001 (PubMed). Further details: Immunohistochemistry

Steiniger, Barth, Herbst, Hartnell, Crocker: "The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone." in: Immunology, Vol. 92, Issue 2, pp. 307-16, 1998 (PubMed).