GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN153484, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
639
5
5
2
2
2
1
1
Host
Mouse
327
171
80
41
16
9
8
2
Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is un-conjugated
32
30
27
14
12
11
11
8
8
8
7
7
6
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro), SDS-PAGE (SDS), Western Blotting (WB)
522
337
188
144
140
119
95
72
57
26
15
13
12
8
8
6
6
5
4
4
3
3
3
3
2
2
1
1
1
1
1
Options
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Immunogen The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Clone 9F9-F9
Isotype IgG2a
Specificity Reactivity is observed against wild type and recombinant forms of GFP. No reaction is seen against RFP.
Purification Protein A purified
Alternative Name GFP
Background Gene Symbol: GFP
Molecular Weight Theoretical MW: 27 kDa
Research Area Tags/Labels
Application Notes Western Blot 1:3000 - 1:30000, Flow Cytometry 1:10 - 1:1000, ELISA 1:80000 - 1:500000, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin 1:1000 - 1:5000, In vitro assay, Dot Blot 1:3000 - 1:30000, SDS-PageUse in In vitro assay reported in scientific literature (PMID 23125142) Use in dot blot reported in scientific literature (PMID 24497578) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153484) anti-Green Fluorescent Protein (GFP) antibody
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153484) Western Blot: GFP Antibody (9F9.F9) [ABIN153484] - Recombinantly expressed GFP in Hel...
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153484) Immunocytochemistry/Immunofluorescence: GFP Antibody (9F9.F9) [ABIN153484] - Analysis...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153484) Western Blot: GFP Antibody (9F9.F9) [ABIN153484] - Lane 1: Molecular Weight Markers. ...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153484) Western Blot: GFP Antibody (9F9.F9) [ABIN153484] - Expression of GFP in CHO-K1 cells ...
SDS-PAGE (SDS) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153484) SDS-Page: GFP Antibody (9F9.F9) [ABIN153484] - SDS PAGE of Mouse anti-GFP antibody. L...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153484) Western Blot: GFP Antibody (9F9.F9) [ABIN153484] - Lane 1: 64pg of recombinant GFP pr...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN153484) Western Blot: GFP Antibody (9F9.F9) [ABIN153484] - Analysis using the Biotin conjugat...
Product cited in: Zusso, Mercanti, Belluti, Di Martino, Pagetta, Marinelli, Brun, Ragazzi, Lo, Stifani, Giusti, Moro: "Phenolic 1,3-diketones attenuate lipopolysaccharide-induced inflammatory response by an alternative magnesium-mediated mechanism." in: British journal of pharmacology, Vol. 174, Issue 10, pp. 1090-1103, 2017 (PubMed). Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Boltengagen, Huang, Boltengagen, Trixl, Lindner, Kremser, Offterdinger, Lusser: "A novel role for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster." in: Nucleic acids research, Vol. 44, Issue 5, pp. 2145-59, 2016 (PubMed). (Sample species: Fruit Fly (Drosophila melanogaster)). Further details: Immunocytochemistry,Immunofluorescence

Song, Sung, Nam, Yoon, Chung, Moffat, Jung, Pak, Kim, Hoe: "A Mercaptoacetamide-Based Class II Histone Deacetylase Inhibitor Increases Dendritic Spine Density via RasGRF1/ERK Pathway." in: Journal of Alzheimer's disease : JAD, Vol. 51, Issue 2, pp. 591-604, 2016 (PubMed).

Bin, Jung, Kim, Chandrasegram, Turlova, Zhu, Gaisano, Sun, Sugita: "Chaperoning of closed syntaxin-3 through Lys46 and Glu59 in domain 1 of Munc18 proteins is indispensable for mast cell exocytosis." in: Journal of cell science, Vol. 128, Issue 10, pp. 1946-60, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Kim, Jeong, Chang: "Extracellular matrix protein reelin regulate dendritic spine density through CaMKII?." in: Neuroscience letters, Vol. 599, pp. 97-101, 2015 (PubMed).

Della Pia, Martinez: "Single domain antibodies as a powerful tool for high quality surface plasmon resonance studies." in: PLoS ONE, Vol. 10, Issue 3, pp. e0124303, 2015 (PubMed).

Pelassa, Corà, Cesano, Monje, Montarolo, Fiumara: "Association of polyalanine and polyglutamine coiled coils mediates expansion disease-related protein aggregation and dysfunction." in: Human molecular genetics, Vol. 23, Issue 13, pp. 3402-20, 2014 (PubMed). (Sample species: Human). Further details: Dot Blot

Ding, Miyazaki, Tsukasa, Matsubara, Yoshimitsu, Takao: "CD133 facilitates epithelial-mesenchymal transition through interaction with the ERK pathway in pancreatic cancer metastasis." in: Molecular cancer, Vol. 13, pp. 15, 2014 (PubMed). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Sunzenauer, Zojer, Brameshuber, Tröls, Weghuber, Stockinger, Schütz: "Determination of binding curves via protein micropatterning in vitro and in living cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 83, Issue 9, pp. 847-54, 2013 (PubMed). Further details: In vitro Assay

Rieusset, Schaller, Unmehopa, Matarazzo, Watrin, Linke, Georges, Bischof, Dijkstra, Bloemsma, Corby, Michel, Wevrick, Zechner, Swaab, Dudley, Bezin, Muscatelli: "Stochastic loss of silencing of the imprinted Ndn/NDN allele, in a mouse model and humans with prader-willi syndrome, has functional consequences." in: PLoS genetics, Vol. 9, Issue 9, pp. e1003752, 2013 (PubMed).

Megill, Lee, DiBattista, Song, Spitzer, Rubinshtein, Habib, Capule, Mayer, Turner, Kirkwood, Yang, Pak, Lee, Hoe: "A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 22, pp. 9306-18, 2013 (PubMed). (Sample species: Firefly). Further details: Immunocytochemistry,Immunofluorescence

Sohn, Lee, Chung, Saavedra, Scott Turner, Pak, Hoe: "Antihypertensive drug Valsartan promotes dendritic spine density by altering AMPA receptor trafficking." in: Biochemical and biophysical research communications, Vol. 439, Issue 4, pp. 464-70, 2013 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Xu, Amiji: "Therapeutic gene delivery and transfection in human pancreatic cancer cells using epidermal growth factor receptor-targeted gelatin nanoparticles." in: Journal of visualized experiments : JoVE, Issue 59, pp. e3612, 2012 (PubMed). Further details: ELISA

Attarwala, Amiji: "Multi-compartmental nanoparticles-in-emulsion formulation for macrophage-specific anti-inflammatory gene delivery." in: Pharmaceutical research, Vol. 29, Issue 6, pp. 1637-49, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: ELISA

Jain, Amiji: "Tuftsin-modified alginate nanoparticles as a noncondensing macrophage-targeted DNA delivery system." in: Biomacromolecules, Vol. 13, Issue 4, pp. 1074-85, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: ELISA

Wei, Schubert, Paquet-Durand, Tanimoto, Chang, Koeppen, Ott, Griesbeck, Seeliger, Euler, Wissinger: "Light-driven calcium signals in mouse cone photoreceptors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 20, pp. 6981-94, 2012 (PubMed). (Sample species: Mouse (Murine)).

Zusso, Methot, Lo, Greenhalgh, David, Stifani: "Regulation of postnatal forebrain amoeboid microglial cell proliferation and development by the transcription factor Runx1." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 33, pp. 11285-98, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Goel, Sienkiewicz, Picciani, Lee, Bhattacharya: "Cochlin induced TREK-1 co-expression and annexin A2 secretion: role in trabecular meshwork cell elongation and motility." in: PLoS ONE, Vol. 6, Issue 8, pp. e23070, 2011 (PubMed).

Aebischer, Sturny, Andrieu, Rieusset, Schaller, Geib, Raoul, Muscatelli: "Necdin protects embryonic motoneurons from programmed cell death." in: PLoS ONE, Vol. 6, Issue 9, pp. e23764, 2011 (PubMed).

Maira, Long, Lee, Rubenstein, Stifani: "Erratum to: Role for TGF-β superfamily signaling in telencephalic GABAergic neuron development." in: Journal of neurodevelopmental disorders, Vol. 2, Issue 1, pp. 47, 2011 (PubMed).