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GLI1 antibody (Zinc Finger Protein GLI1) (C-Term)

Details for Product anti-GLI1 Antibody No. ABIN153487, Supplier: Log in to see
Antigen
  • AV235269
  • GLI
  • Gli
  • Gli-1
  • gli1.1
  • Zfp-5
  • Zfp5
Alternatives
anti-Mouse (Murine) GLI1 antibody for ELISA
Epitope
C-Term, AA 800-850
41
17
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14
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12
6
5
5
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3
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
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52
19
4
3
2
2
2
2
1
Host
Rabbit
96
85
4
3
1
Clonality
Polyclonal
Conjugate
This GLI1 antibody is un-conjugated
7
7
7
4
4
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3
3
3
3
3
2
1
1
1
1
1
1
1
1
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1
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
156
57
47
46
33
20
16
12
5
2
2
2
Supplier
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Immunogen A synthetic peptide made toward the C-terminus portion of the human Gli1 protein (between residues 800-850) [UniProt P08151]
Specificity This is directed against Gli1 from mouse brain. No reaction occurs with human or mouse Gli2 or Gli3.
Purification Immunogen affinity purified
Alternative Name GLI-1 (GLI1 Antibody Abstract)
Background Gene Symbol: GLI1
Gene ID 2735, 14632
UniProt P08151
Pathways Hedgehog Signaling
Application Notes Western Blot 1:2000-1:10000, Chromatin Immunoprecipitation 1:10-1:500, ELISA 1:20000-1:100000, Immunohistochemistry 1:600, Immunohistochemistry-Paraffin 1:600, Flow (Intracellular) 1-2 μg/million cells
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for Gli1 antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Protocol Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only
Format Liquid
Concentration 1.05 mg/mL
Buffer PBS, 30 % glycerol
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-GLI1 antibody (Zinc Finger Protein GLI1) (C-Term) (ABIN153487) anti-Zinc Finger Protein GLI1 (GLI1) (AA 800-850), (C-Term) antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-GLI1 antibody (Zinc Finger Protein GLI1) (C-Term) (ABIN153487) Immunohistochemistry-Paraffin: GLI-1 Antibody [ABIN153487] - IHC-P analysis of a form...
 image for anti-GLI1 antibody (Zinc Finger Protein GLI1) (C-Term) (ABIN153487) Flow (Intracellular): GLI-1 Antibody [ABIN153487] - intracellular staining for GLI-1 ...
Product cited in: Comba, Almada, Tolosa, Iguchi, Marks, Vara Messler, Silva, Fernandez-Barrena, Enriquez-Hesles, Vrabel, Botta, Di Marcotulio, Ellenrieder, Eynard, Pasqualini, Fernandez-Zapico: "Nuclear Factor of Activated T Cells-dependent Down-regulation of the Transcription Factor Glioma-associated Protein 1 (GLI1) Underlies the Growth Inhibitory Properties of Arachidonic Acid." in: The Journal of biological chemistry, Vol. 291, Issue 4, pp. 1933-47, 2016 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Valverde, Pereira, Dias, Guimarães, Ramos, Santos, Gurgel Rocha: "Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation." in: Tumour biology, Vol. 37, Issue 7, pp. 9233-41, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Vidal, Lourenço, Soares, Gurgel, Studart, Valverde, Araújo, Ramos, Xavier, Dos Santos: "The sonic hedgehog signaling pathway contributes to the development of salivary gland neoplasms regardless of perineural infiltration." in: Tumour biology, Vol. 37, Issue 7, pp. 9587-601, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Wu, Ingram, Tolosa, Vera, Li, Kim, Ma, Spyropoulos, Beharry, Huang, Fernandez-Zapico, Cai: "Gli Transcription Factors Mediate the Oncogenic Transformation of Prostate Basal Cells Induced by a Kras-Androgen Receptor Axis." in: The Journal of biological chemistry, Vol. 291, Issue 49, pp. 25749-25760, 2016 (PubMed). Method employed by authors: Immunofluorescence (IF)

Jackson, Smith, Amarsaikhan, Han, Neil, Boi, Vrabel, Tolosa, Almada, Fernandez-Zapico, Elsawa: "Modulation of the IL-6 Receptor α Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenström Macroglobulinemia Cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 6, pp. 2908-16, 2015 (PubMed).

Yeddula, Xia, Ke, Beumer, Verma: "Screening for tumor suppressors: Loss of ephrin receptor A2 cooperates with oncogenic KRas in promoting lung adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 47, pp. E6476-85, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Huang, Walter, Hayes, Onaitis: "Hedgehog-GLI signaling inhibition suppresses tumor growth in squamous lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 20, Issue 6, pp. 1566-75, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Nye, Almada, Fernandez-Barrena, Marks, Elsawa, Vrabel, Tolosa, Ellenrieder, Fernandez-Zapico: "The transcription factor GLI1 interacts with SMAD proteins to modulate transforming growth factor β-induced gene expression in a p300/CREB-binding protein-associated factor (PCAF)-dependent manner." in: The Journal of biological chemistry, Vol. 289, Issue 22, pp. 15495-506, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation

Jiang, Li, He, Wang, Li, Gao: "Expression of Gli1 and Wnt2B correlates with progression and clinical outcome of pancreatic cancer." in: International journal of clinical and experimental pathology, Vol. 7, Issue 7, pp. 4531-8, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Regard, Malhotra, Gvozdenovic-Jeremic, Josey, Chen, Weinstein, Lu, Shore, Kaplan, Yang: "Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification." in: Nature medicine, Vol. 19, Issue 11, pp. 1505-12, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Mazumdar, Sandhu, Qadan, DeVecchio, Magloire, Agyeman, Li, Houghton: "Hedgehog signaling regulates telomerase reverse transcriptase in human cancer cells." in: PLoS ONE, Vol. 8, Issue 9, pp. e75253, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation

Karlou, Lu, Wu, Maity, Tzelepi, Navone, Hoang, Logothetis, Efstathiou: "Hedgehog signaling inhibition by the small molecule smoothened inhibitor GDC-0449 in the bone forming prostate cancer xenograft MDA PCa 118b." in: The Prostate, Vol. 72, Issue 15, pp. 1638-47, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Mazumdar, DeVecchio, Shi, Jones, Agyeman, Houghton: "Hedgehog signaling drives cellular survival in human colon carcinoma cells." in: Cancer research, Vol. 71, Issue 3, pp. 1092-102, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Tzelepi, Karlou, Wen, Hoang, Logothetis, Troncoso, Efstathiou: "Expression of hedgehog pathway components in prostate carcinoma microenvironment: shifting the balance towards autocrine signalling." in: Histopathology, Vol. 58, Issue 7, pp. 1037-47, 2011 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Mazumdar, Devecchio, Agyeman, Shi, Houghton: "Blocking Hedgehog survival signaling at the level of the GLI genes induces DNA damage and extensive cell death in human colon carcinoma cells." in: Cancer research, Vol. 71, Issue 17, pp. 5904-14, 2011 (PubMed).

Qualtrough, Buda, Gaffield, Williams, Paraskeva: "Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment." in: International journal of cancer, Vol. 110, Issue 6, pp. 831-7, 2004 (PubMed).

Niemann, Unden, Lyle, Zouboulis, Toftgård, Watt: "Indian hedgehog and beta-catenin signaling: role in the sebaceous lineage of normal and neoplastic mammalian epidermis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100 Suppl 1, pp. 11873-80, 2003 (PubMed). (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation

Reference: Breunig, Sarkisian, Arellano, Morozov, Ayoub, Sojitra, Wang, Flavell, Rakic, Town: "Primary cilia regulate hippocampal neurogenesis by mediating sonic hedgehog signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 35, pp. 13127-32, 2008 (PubMed).

Wetmore, Eberhart, Curran: "The normal patched allele is expressed in medulloblastomas from mice with heterozygous germ-line mutation of patched." in: Cancer research, Vol. 60, Issue 8, pp. 2239-46, 2000 (PubMed).

Park, Bai, Platt, Matise, Beeghly, Hui, Nakashima, Joyner: "Mouse Gli1 mutants are viable but have defects in SHH signaling in combination with a Gli2 mutation." in: Development (Cambridge, England), Vol. 127, Issue 8, pp. 1593-605, 2000 (PubMed).