Zinc Finger Protein GLI1 (GLI1) antibody

Antigen: Zinc Finger Protein GLI1 (GLI1)

Synonyms: GLI

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA

Catalog no.: ABIN153487

Additional Information

Alternative name: GLI1

Gene ID: 2735, 14632

Immunogen: Synthetic peptide representing the C-terminus of the mouse protein (residues 803-818)according to NP_005260.

Cross-Reactivity: Human, Mouse (Murine)

Format: Serum

Description: This antiserum was produced against a peptide corresponding to the carboxy-terminalregion of the mouse Gli-1 protein. This region is not conserved among other gli familymembers, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as',glioma-associated oncogene', amplified in malignant gliomas. Analysis ofthe cloned gene demonstrates that the gene contains 5 repeats of zinc-fingersequences, which places gli in the family of Kruppel (Kr) zinc finger proteins. Northernanalysis reveals that GLI is expressed in embryonal carcinoma cells but not in mostadult tissue. GLI has been localized to 12q13-q14.3 by Southern blot analysis. Onmouse the gene is located on chromosome 10. In mice, 3 zinc finger transcriptionfactors, Gli-1, Gl-i2 and Gli-3, have been implicated in the transduction of Sonichedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similarexpression patterns. Gli1 expression is central and probably sufficient for tumordevelopment in humans. Alternate Names: Anti-GLI antibody, Anti-Glioma associated oncogene antibody, Anti-Glioma associatedoncogene homolog 1 (zinc finger protein) antibody, Anti-Oncogene GLI antibody,Anti-Zinc finger protein GLI1 antibody.

Specificity: This antibody is directed against Gli1 from mouse brain. No reaction occurs with humanor mouse Gli2 or Gli3. Species Reactivity: Reacts with human and mouse. Localization: Cytoplasmic and Nuclear.

Application Details

Application Notes: Suggested working dilutions: ELISA 1:20,000 - 1:100,000,immunohistochemistry 1:500 - 1:2,000,Western Blot 1:2,000 - 1:10,000,000-1:10,000

Purification: Serum

Buffer: 0.15M Sodium Chloride, 0.02M Potassium Phosphate [pH 7]. Preservative: 0.1% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Growth Factors

Restrictions: For Research Use only

Images

anti-Zinc Finger Protein GLI1 (GLI1) antibody

Publications

Kinzler, Ruppert, Bigner et al.: "The GLI gene is a member of the Kruppel family of zinc finger proteins." in: Nature, Vol. 332, Issue 6162, pp. 371-4, 1988 (PubMed).

Kinzler, Bigner, Bigner et al.: "Identification of an amplified, highly expressed gene in a human glioma." in: Science (New York, N.Y.), Vol. 236, Issue 4797, pp. 70-3, 1987 (PubMed).

Dahmane, Lee, Robins et al.: "Activation of the transcription factor Gli1 and the Sonic hedgehog signalling pathway in skin tumours." in: Nature, Vol. 389, Issue 6653, pp. 876-81, 1997 (PubMed).

Hall, Hooper, Finger: "Expression of sonic hedgehog, patched, and Gli1 in developing taste papillae of the mouse." in: The Journal of comparative neurology, Vol. 406, Issue 2, pp. 143-55, 1999 (PubMed).

Ghali, Wong, Green et al.: "Gli1 protein is expressed in basal cell carcinomas, outer root sheath keratinocytes and a subpopulation of mesenchymal cells in normal human skin." in: The Journal of investigative dermatology, Vol. 113, Issue 4, pp. 595-9, 1999 (PubMed).

Park, Bai, Platt et al.: "Mouse Gli1 mutants are viable but have defects in SHH signaling in combination with a Gli2 mutation." in: Development (Cambridge, England), Vol. 127, Issue 8, pp. 1593-605, 2000 (PubMed).

Wetmore, Eberhart, Curran: "The normal patched allele is expressed in medulloblastomas from mice with heterozygous germ-line mutation of patched." in: Cancer research, Vol. 60, Issue 8, pp. 2239-46, 2000 (PubMed).

Qualtrough, Buda, Gaffield et al.: "Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment." in: International journal of cancer. Journal international du cancer, Vol. 110, Issue 6, pp. 831-7, 2004 (PubMed).

Breunig, Sarkisian, Arellano et al.: "Primary cilia regulate hippocampal neurogenesis by mediating sonic hedgehog signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 35, pp. 13127-32, 2008 (PubMed).