Y Box Binding Protein 1 (YBX1) (N-Term) antibody

Antigen: Y Box Binding Protein 1 (YBX1)

Synonyms: Yb1, YB1, BP-8, CSDB, DBPB, YB-1, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250, ybx1, frgy1, nsep1, MGC52597, MGC130847

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN155053

Additional Information

Alternative name: YB-1 (N-Term)

Gene ID: 4904

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Members of Y-box binding protein family all contain a DNA binding domain, called the cold shock domain, comprising of 80 amino acids, which is highly conserved from prokaryotes to eukaryotes. Y-box binding protein-1 (YB-1) is a prototypic member of this family and was originally isolated as a DNA binding protein recognizing the Y-box sequence within the MHC class II gene promoter region. This box is found in the promoter region of several genes involved in positive regulation of cell cycle, such as proliferating cell nuclear antigen, DNA topoisomerase IIa, DNA-polymerase-alpha and cyclins. Consequently, YB-1 is thought to play crucial roles for cell proliferation and carcinogenesis. YB-1 has been reported to be a prognostic marker of breast cancer, ovarian cancer, lung cancer, thyroid cancer, and synovial sarcoma. These findings indicate the significance of YB-1 for the progression of malignant diseases. Alternative names: BP-8, CSDA2, CSDB, DBPB, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP-1, NSEP1, YB-1, YB1, YBX1, DNA-binding protein B, Y box-binding protein I, nuclease sensitive element binding protein 1.

Application Details

Application Notes: WB: 1:1000. IHC (p): 1:50 to 1:200 depending on type of tissue

Concentration: 200ug/ml

Storage: Store aliquots at -20 C. Avoid repeated thawing and freezing

Research Area: Cancer

Restrictions: For Research Use only

Images

Western Blot

IHC

Publications

Publications: Krohn R, Raffetseder U, Bot I et al.: "Y-Box Binding Protein-1 Controls CC Chemokine Ligand-5 (CCL5) Expression in Smooth Muscle Cells and Contributes to Neointima Formation in Atherosclerosis-Prone Mice" in: Circulation, Vol. Epub ahead of print, 2007 (PubMed).

Frye, Halfter, Djudjaj et al.: "Y-box protein-1 is actively secreted through a non-classical pathway and acts as an extracellular mitogen." in: EMBO reports, Vol. 10, Issue 7, pp. 783-9, 2009 (PubMed).

Rauen, Raffetseder, Frye et al.: "YB-1 acts as ligand for Notch-3 receptors and modulates receptor activation." in: The Journal of biological chemistry, 2009 (PubMed).

Mertens, Martin, Frye et al.: "Rat Mrp2 gene expression is regulated by an interleukin-1β-stimulated biphasic response with enhanced transcription and subcellular shuttling of YB-1." in: European journal of cell biology, 2012 (PubMed).