POU2F1 antibody

Catalog No. ABIN155078

Product Details anti-POU2F1 Antibody

Target: POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects Oct-1.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 727 of Human Oct-1

Application Details

Application Notes: Western blotting: 0.5-4 μg/mL. Recommended Positive Control: Jurkat cell lysate. Immunohistochemistry: 10-20 μg/mL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS pH 7.2, containing 30 % Glycerol, 0.5 % BSA as stabilizer and 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted in aliquots at -20 °C to -70 °C.

Target Details for POU2F1

Target: POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))

Alternative Name: POU2F1 / OCT1 (POU2F1 Products)

Synonyms: OCT1 antibody, OTF1 antibody, oct-1B antibody, POU2F1 antibody, 2810482H01Rik antibody, NF-A1 antibody, Oct-1 antibody, Oct1 antibody, Otf-1 antibody, Otf1 antibody, chOCT-1 antibody, oct-1 antibody, oct1 antibody, pou2f1r antibody, fc07d06 antibody, wu:fc07d06 antibody, zgc:158360 antibody, POU class 2 homeobox 1 antibody, POU domain, class 2, transcription factor 1 antibody, POU class 2 homeobox 1 S homeolog antibody, POU class 2 homeobox 1b antibody, POU2F1 antibody, Pou2f1 antibody, pou2f1.S antibody, pou2f1b antibody

Background: Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.Synonyms: NF-A1, OTF1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1, Otf-1, POU domain class 2 transcription factor 1

Gene ID: 5451

NCBI Accession: NP_001185712

UniProt: P14859