HMOX1
Reactivity: Human
WB, IHC, IP, ELISA, ICC, IF
Host: Mouse
Monoclonal
1F12-A6
FITC
Application Notes
Western Blot: 0.5-2 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry: 10-20 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
PBS, 0.5 % BSA, 30 % Glycerol, 0.01 % Thimerosal
Preservative
Sodium azide, Thimerosal (Merthiolate)
Precaution of Use
This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C/-80 °C
Storage Comment
Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
Heme oxygenase-1 (HO-1) or HSP32 is the inducible isoform of heme oxygenase which catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, iron and biliverdin that is immediately reduced to bilirubin. To date, three heme oxygenase isoforms HO-1, HO-2 and HO-3 have been identified. HO-1, also known as Hsp32, a major heat shock/stress response protein, is ubiquitous and its mRNA as well as its activity can be increased several-fold by heme, other metalloporphyrins, transition metals and stimuli that induce cellular stress. The 5'-untranslated region (UTR) of HO-1 has several consensus regulatory elements that include sites for activator protein 1 (AP-1), metal responsive element (MRE), oncogene c-myc/max heterodimer binding site (Myc/Max), antioxidant response element (ARE) and GC box binding (Sp1). HO-1 expression has been shown to increase in benign postatic hyperplasia (BPH) and malignant prostate tissue suggesting a role for this stress protein in the pathogenesis of BPH and prostate cancer.Synonyms: HO, HO-1, HO1