Podoplanin antibody (Biotin)

Catalog No. ABIN155160

Product Details anti-Podoplanin Antibody (Biotin)

Target: Podoplanin (PDPN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with Podoplanin (gp36). The unconjugated monoclonal antibody will detect native Human Podoplanin in Western Blot and on the surface of Human LEC.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Podoplanin-expressing MDCK cells.

Clone: 18H5

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: use at 2-5 μg/mL together with the appropriate secondary reagents.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS, 0.02 % Sodium Azide, BSA (50x)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

References for anti-Podoplanin antibody (Biotin) (ABIN155160)

Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." in: Circulation, Vol. 119, Issue 2, pp. 281-9, (2009) (PubMed).

Turner, Langley, Johnston, Gentil, Ford, Wu, Graham, Sharpley, Slatko, Pearlman, Taylor: "Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22364-78, (2009) (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, (2007) (PubMed).

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin (PDPN Products)

Synonyms: PDPN antibody, AGGRUS antibody, GP36 antibody, GP40 antibody, Gp38 antibody, HT1A-1 antibody, OTS8 antibody, PA2.26 antibody, T1A antibody, T1A-2 antibody, OTS-8 antibody, RANDAM-2 antibody, T1-alpha antibody, T1a antibody, T1alpha antibody, E11 antibody, RTI40 antibody, podoplanin antibody, PDPN antibody, Pdpn antibody

Background: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

Gene ID: 10630

NCBI Accession: NP_001006625

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport