Podoplanin antibody

Catalog No. ABIN155169

Product Details anti-Podoplanin Antibody

Target: Podoplanin (PDPN)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The monoclonal antibody will detect Podoplanin on the surface of lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. The antibody does not cross-react with Human Podoplanin.

Purification: Protein G Chromatography

Immunogen: Murine thymic stromal cell line

Clone: RTD4E10

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (use diluted 1-10 μg/mL IgG). Works well with paraffin sections. Western blot (use 1-10 μg/mL IgG, will detect a 36-40 kDa band). Immunoprecipitation (use 1-5 μg IgG per 1 mLlysate or reaction volume).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4, without preservatives or stabilizers

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. After reconstitution store the antibody (in aliquots) at -20 °C.

References for anti-Podoplanin antibody (ABIN155169)

Uhrin, Zaujec, Breuss, Olcaydu, Chrenek, Stockinger, Fuertbauer, Moser, Haiko, Fässler, Alitalo, Binder, Kerjaschki: "Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation." in: Blood, Vol. 115, Issue 19, pp. 3997-4005, (2010) (PubMed).

DAmico, Jones, Nye, Sapienza, Ramjuan, Reynolds, Robinson, Kostourou, Martinez, Aubyn, Grose, Thomas, Spencer-Dene, Zicha, Davies, Tybulewicz, Hodivala-Dilke: "Regulation of lymphatic-blood vessel separation by endothelial Rac1." in: Development (Cambridge, England), Vol. 136, Issue 23, pp. 4043-53, (2009) (PubMed).

NOYES, DOMM, WILLSLC: "Regulation of erythropoiesis. I. Erythropoietin assay as a clinical tool." in: Blood, Vol. 20, pp. 9-18 (PubMed).

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin (PDPN Products)

Synonyms: PDPN antibody, AGGRUS antibody, GP36 antibody, GP40 antibody, Gp38 antibody, HT1A-1 antibody, OTS8 antibody, PA2.26 antibody, T1A antibody, T1A-2 antibody, OTS-8 antibody, RANDAM-2 antibody, T1-alpha antibody, T1a antibody, T1alpha antibody, E11 antibody, RTI40 antibody, podoplanin antibody, PDPN antibody, Pdpn antibody

Background: Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

Gene ID: 14726

NCBI Accession: NP_034459

UniProt: Q62011

Pathways: Dicarboxylic Acid Transport